重金属及其交互作用对小麦幼苗中金属含量的影响

有关复合污染的研究,在国内外已引起普遍重视,因为人们早已发现金属间的联合作用,可以大大改变某元素的生物活性或毒性,要比单个成分的作用更为重要。因此,研究环境中各种元素或化合物之间存在的拮抗作用和协同     

Application of β-glucuronidase (GusA) as an effective reporter for extremely acidophilic Acidithiobacillus ferrooxidans

Applied Microbiology and Biotechnology - Acidithiobacillus ferrooxidans is a model organism for investigating metal sulfide bioleaching. The regulatory mechanism of gene expression by metabolizing...     

崇义客家梯田生态系统可持续发展综合评价

崇义客家梯田是中国最大的客家梯田,为当地客家人提供了多项生态服务功能。综合评价崇义客家梯田生态系统的可持续性,有助于人们合理开发和利用其资源。运用综合加权函数、协调度模型和发展障碍度模型对崇义客家梯田生态系统可持续发展现状、可持续发展潜能以及影响其可持续发展的障碍因素作了分析诊断。结果表明:1990—2014年间,崇义客家梯田生态系统可持续发展综合指数增长了2.22倍。各指标间的协调度均大于0.65,指标间协调发展的程度良好,崇义客家梯田生态系统可持续发展存在较大潜能。影响崇义客家梯田生态系统可持续发展的主要单项障碍性因素有服务业产值、农民受教育程度、农民恩格尔系数和农田受灾率4项,他们的障碍度值分别为0.17、0.17、0.15和0.13。直观的数据反映了崇义客家梯田生态系统的可持续发展情况,也为当地政府制定崇义客家梯田生态系统可持续发展对策提供了可靠的支撑。     

基于MEMS技术的毛细管电泳芯片

基于MEMS技术的毛细管电泳芯片具有体积小、成本低、便于携带、分析速度快、所需样品和试剂少等优点,是一种新型的微型分析实验装置。介绍该芯片的结构、进样方式、材料选取、制作工艺、键合方法、样品检测方法等方面的研究进展和存在的问题,展望了应用前景 。     

A small molecule toll-like receptor antagonist rescues α-synuclein fibril pathology

The propagation and accumulation of pathological α-synuclein protein is thought to underlie the clinical symptoms of the neurodegenerative movement disorder Parkinson’s disease (PD). Consequently, there is significant interest in identifying the mechanisms that contribute to α-synuclein pathology, as these may inform therapeutic targets for the treatment of PD. One protein that appears to contribute to α-synuclein pathology is the innate immune pathogen recognition receptor, toll-like receptor 2 (TLR2). TLR2 is expressed on neurons, and its activation results in the accumulation of α-synuclein protein; however, the precise mechanism by which TLR2 contributes to α-synuclein pathology is unclear. Herein we demonstrate using human cell models that neuronal TLR2 activation acutely impairs the autophagy lysosomal pathway and markedly potentiates α-synuclein pathology seeded with α-synuclein preformed fibrils. Moreover, α-synuclein pathology could be ameliorated with a novel small molecule TLR2 inhibitor, including in induced pluripotent stem cell-derived neurons from a patient with PD. These results provide further insight into how TLR2 activation may promote α-synuclein pathology in PD and support that TLR2 may be a potential therapeutic target for the treatment of PD.     

MicroRNA-429 induces tumorigenesis of human non-small cell lung cancer cells and targets multiple tumor suppressor genes

Lung cancer is the major cause of cancer death globally. MicroRNAs are evolutionally conserved small noncoding RNAs that are critical for the regulation of gene expression. Aberrant expression of microRNA (miRNA) has been implicated in cancer initiation and progression. In this study, we demonstrated that the expression of miR-429 are often upregulated in non-small cell lung cancer (NSCLC) compared with normal lung tissues, and its expression level is also increased in NSCLC cell lines compared with normal lung cells. Overexpression of miR-429 in A549 NSCLC cells significantly promoted cell proliferation, migration and invasion, whereas inhibition of miR-429 inhibits these effects. Furthermore, we demonstrated that miR-429 down-regulates PTEN, RASSF8 and TIMP2 expression by directly targeting the 3′-untranslated region of these target genes. Taken together, our results suggest that miR-429 plays an important role in promoting the proliferation and metastasis of NSCLC cells and is a potential target for NSCLC therapy.     

农作物Cd耐性的种内和种间差异Ⅱ．种内差

对２８个小麦、１４个大豆品种（或品系）的幼苗培养试验表明,作物对Ｃｄ的耐性具有明显的种内差异;耐性品种与其吸收Ｃｄ相对较少或向地上部运移比例较低有关;部分品种虽吸收Ｃｄ量较高,但仍能正常生长,推断其具有使Ｃｄ转化为活性较低形态,从而减轻毒害的机制．     

菟丝子资源现状与栽培技术分析

通过对菟丝子植物资源和栽培技术的研究,为菟丝子的规范化种植和可持续利用提供依据.     

Effects of 1-methyltryptophan stereoisomers on IDO2 enzyme activity and IDO2-mediated arrest of human T cell proliferation

IDO2 is a newly discovered enzyme with 43?% similarity to classical IDO (IDO1) protein and shares the same critical catalytic residues. IDO1 catalyzes the initial and rate-limiting step in the degradation of tryptophan and is a key enzyme in mediating tumor immune tolerance via arrest of T cell proliferation. The role of IDO2 in human T cell immunity remains controversial. Here, we demonstrate that similar to IDO1, IDO2 also degrades tryptophan into kynurenine and is inhibited more efficiently by Levo-1-methyl tryptophan (L-1MT), an IDO1 competitive inhibitor, than by dextro-methyl tryptophan (D-1MT). Although IDO2 enzyme activity is weaker than IDO1, it is less sensitive to 1-MT inhibition than IDO1. Moreover, our results indicate that human CD4⁺ and CD8⁺ T cell proliferation was inhibited by IDO2, but both L-1MT and D-1MT could not reverse IDO2-mediated arrest of cell proliferation, even at high concentrations. These data indicate that IDO2 is an inhibitory mechanism in human T cell proliferation and support efforts to develop more effective IDO1 and IDO2 inhibitors in order to overcome IDO-mediated immune tolerance.