推拿对慢性应激大鼠抑郁行为的影响及其机制

目的:探讨推拿对慢性应激大鼠抑郁行为的影响及其作用机制.方法:制备慢性轻度不可预见性的应激大鼠模型[1-2],造模21 d后,进行推拿治疗14 d.分组:空白对照组、模型组、推拿组、氟西汀组,每组10只.每日推拿膀胱经重要穴位10 min(间隔2 min,共2次).通过体质量检测、旷场、糖水消耗实验和水迷宫实验评价抑郁...     

家蚕抗真菌因子BmSPI39对球孢白僵菌入侵的表达响应

[目的]制备家蚕Bombyx mori抗真菌因子BmSPI39的多克隆抗体,分析BmSPI39对球孢白僵菌Beauveria bassiana入侵的表达响应.[方法]利用原核表达和固定化镍离子亲和层析技术获得高纯度的BmSPI39重组蛋白,利用胶内活性染色检测重组蛋白BmSPI39对枯草杆菌Bacillus subti...     

The progress of multimodal imaging combination and subregion based radiomics research of cancers

In recent years, with the standardization of radiomics methods; development of tools; and popularization of the concept, radiomics has been widely used in all aspects of tumor diagnosis; treatment; and prognosis. As the study of radiomics in cancer has become more advanced, the currently used methods have revealed their shortcomings. The performance of cancer radiomics based on single-modality medical images, which based on their imaging principles, only partially reflects tumor information, has been necessarily compromised. Using the whole tumor as a region of interest to extract radiomic features inevitably leads to the loss of intra-tumoral heterogeneity of, which also affects the performance of radiomics. Radiomics of multimodal images extracts various aspects of information from images of each modality and then integrates them together for model construction; thus, avoiding missing information. Subregional segmentation based on multimodal medical image combinations allows radiomics features acquired from subregions to retain tumor heterogeneity, further improving the performance of radiomics. In this review, we provide a detailed summary of the current research on the radiomics of multimodal images of cancer and tumor subregion-based radiomics, and then raised some of the research problems and also provide a thorough discussion on these issues.     

Assessing the risk of resistance to cationic biocides incorporating realism-based and biophysical approaches

The control of microorganisms is a key objective in disease prevention and in medical, industrial, domestic, and food-production environments. Whilst the effectiveness of biocides in these contexts is well-evidenced, debate continues about the resistance risks associated with their use. This has driven an increased regulatory burden, which in turn could result in a reduction of both the deployment of current biocides and the development of new compounds and formulas. Efforts to balance risk and benefit are therefore of critical importance and should be underpinned by realistic methods and a multi-disciplinary approach, and through objective and critical analyses of the literature. The current literature on this topic can be difficult to navigate. Much of the evidence for potential issues of resistance generation by biocides is based on either correlation analysis of isolated bacteria, where reports of treatment failure are generally uncommon, or laboratory studies that do not necessarily represent real biocide applications. This is complicated by inconsistencies in the definition of the term resistance. Similar uncertainties also apply to cross-resistance between biocides and antibiotics. Risk assessment studies that can better inform practice are required. The resulting knowledge can be utilised by multiple stakeholders including those tasked with new product development, regulatory authorities, clinical practitioners, and the public. This review considers current evidence for resistance and cross-resistance and outlines efforts to increase realism in risk assessment. This is done in the background of the discussion of the mode of application of biocides and the demonstrable benefits as well as the potential risks.     

调控巨噬细胞迁移抑制因子基因表达的影响因素

巨噬细胞迁移抑制因子(macrophage migration inhibitory factor, MIF)是一种广泛表达的多效性细胞因子,参与多种炎症和免疫疾病的过程并在其中发挥重要作用,是许多疾病的生物标志物或治疗靶点。MIF基因在系统发育中高度保守,在其启动子区有多种不同转录因子的特定结合位点,借此调节MIF的表达。MIF在细胞内外均发挥作用,且MIF是组成型表达。因此,研究调控MIF基因表达和刺激MIF分泌的相关因素具有重要意义。本文通过对MIF基因和MIF启动子上的结合位点的简述,对影响MIF基因表达的相关因素进行总结和归类。根据与MIF基因结合的方式,可分为：(1)与MIF基因启动子特定位点结合,改变转录活性;(2)与MIF CATT5-8微卫星重复序列结合,改变高表达MIF等位基因;(3)非编码RNA调控MIF表达;(4)影响MIF分泌的相关因素。通过对这4类调控MIF基因表达的相关因素的综述,进而认识MIF基因表达的调控机制和影响因素,以期对其治疗相关疾病提供理论基础。     

新疆翠雀花属三新种

该文描述了在新疆维吾尔自治区发现的毛茛科翠雀花属三新种,包括隶属于密花翠雀花组高翠雀花亚组的二新种,即布尔津翠雀花(Delphinium buerjinense W.T.Wang&Z.Z.Yang)和尼勒克翠雀花(D.nilekeense W.T.Wang&Z.Z.Yang),以及隶属于翠雀花组须花翠雀花亚组的一新种,...     

甘肃省大豆品种生育期组的划分及地理分布研究

甘肃省地域广大,地形复杂,气候多样,大豆品种资源丰富,但甘肃各地大豆品种生育期生态类型并不明确.本试验以25份MG 0～Ⅴ的北美大豆生育期组标准品种为对照,将从甘肃各地收集到的151个大豆品种在甘肃境内5个生态区进行生育期性状鉴定和生育期组划分.结果 表明,甘肃地区种植的大豆品种的生育期组在MG 00和MGⅥ之间.在所...     

浙江天童太白山不同群落植物构型比较

植物构型是植株构件在空间上的分配方式,反映了植物对环境的响应策略。通过对浙江天童太白山海拔差异很小的栲树(Castanopsis fargesii)、小叶青冈(Cyclobalanopsis gracilis)和云山青冈(Cyclobalanopsis sessilifolia)群落类型中所有植株的树高、树冠厚度、树冠面积、叶片聚集度、枝下高和距地45 cm基径等植物构型性状,以及树冠曝光指数、土壤含水率、空气温湿度、土壤p H值和风速6个环境因子的测定,分别分析乔灌木层植物构型性状及性状关系在3个群落间的变化规律。结果表明:(1)从栲树到小叶青冈至云山青冈群落,灌木层的树高、树冠厚度、枝下高和距地45 cm基径增加,叶片聚集度减小;乔木层的树高、树冠厚度、树冠面积、枝下高和距地45 cm基径均减小,叶片聚集度增大;(2)3个群落灌木层构型性状间显著相关(P0.001),而乔木层只在中低海拔群落存在相关性;(3)从栲树到小叶青冈至云山青冈群落,乔灌木层的冠层曝光指数显著增加(P0.05);(4)多元逐步回归表明,树冠曝光指数对灌木层构型性状变异的贡献最大,而风速、土壤含水率和p H值对乔木构型性状的变异起主导作用。综上得知,天童太白山乔灌木植物在不同群落间存在构型分异,植物对光资源的竞争是引起灌木构型在不同群落间变化的主要驱动因子,而对乔木植物,其构型变化更多受到风速和土壤含水量的影响。