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81.
通过酶学手段在β-内酰胺酶启动子间隔序列中造成C-17的缺失突变,以氨苄青霉素为底物,检测突变前后β-内酰胺酶活力变化,并作了细菌的Amp耐受性试验,实验结果表明,C-17的缺失突变使启动子强度增加约60%,含有突变启动子的细菌对Amp的抗性明显增强,突变体生长的半抑制浓度为280μg/ml,而在此浓度下野生型菌体吸光度只有突变体的50%左右,对启动子强度增加的原因进行了讨论。 相似文献
82.
在大肠杆菌中表达了人肾液泡型H ̄+-ATPase58kD亚基的基因,利用聚合酶链式反应(PCR)得到了58kD亚基的编码片段.直接将PCR产物连接到PET载体上表达.SDS聚丙烯酰胺凝胶电泳和蛋白质印迹分析表明58kD亚基的基因得到高效表达.表达产物可达细菌细胞质蛋白的50%. 相似文献
83.
The interfering effects of copper, zinc, and cobalt on the uptake of mugineic acid-ferric complex were studied in barley ( Hordeum vulgare , cv. Minorimugi) grown in nutrient solution. Short-term uptake experiments of 3 h were performed utilizing both ionic and mugineic acid-complex forms of each metal at two different concentrations. Copper was most effective in decreasing iron uptake when added in an ionic form at either concentration. The inhibition order at higher concentrations followed Cu(II) > Zn(II) ≥ Co(II), Co(III), which is consistent with the stability constants of these metal complexes with mugineic acid. The displacement of iron from its mugineic acid complex by these metals is suggested as a probable explanation for the decreased iron uptake. The inhibitory effect of metal complexes with mugineic acid on iron uptake was only found in cases with higher concentrations of Cu(II) and Zn(II) complexes. Deformation of the specific iron transport system in the plasma membrane due to their adsorption may be responsible for this effect. 相似文献
84.
Since cytoplasmic Ca2+ levels are reported to regulate neurite elongation, we tested whether calcium-activated kinases might be necessary for growth cone motility and neurite elongation in explant cultures of goldfish retina. Kinase inhibitors and activators were locally applied by micropipette to retinal growth cones and the responses were observed via phase-contrast videomicroscopy. In some cases, growth rates were also quantifed over several hours after general application in the medium. The selective inhibitors of protein kinase C, calphostin C (0.1–1 μM) and chelerythrin (up to 50 μM), caused no obvious changes in growth cones or neurite elongation, and activators of PKC (phorbols, arachidonic acid, and diacylglycerol) also were generally without effects, although phorbols slowed the growth rate. Inhibitors of protein kinase A and tyrosine kinases also produced no obvious effects. The calmodulin antagonists, calmidazolium (0.1 μM), trifluoperazine (100 μM), and CGS9343B (50 μM), however, caused a reversible growth cone arrest with loss of filopodia and lamellipodia. The growth cone became a club-shaped swelling which sometimes moved a short distance back the shaft, leaving evacuated filaments at points of strong filopodial attachments. A similar reversible growth cone arrest occurred with the general kinase inhibitors: H7 at 200 but not at 100 μM, and staurosporine at 100 but not 10 nM, suggesting possible involvement of a calmodulin-dependent kinase (camK) rather than PKC. The selective inhibitor of camKII, KN-62 (tested up to 50 μM), produced no effects but the specific myosin light-chain kinase (MLCK) inhibitors ML-7 (3–5 μM) and ML-9 (5–10 μM) reversibly reproduced the effect, suggesting that MLCK rather than camKII is necessary for growth cone motility. The MLCK inhibitors' effects both on growth cone morphology and on F-actin filaments (rhodamine-phalloidin staining) were similar to those caused by cytochalasin D (5 μM), and are discussed in light of findings that inhibiting MLCK disrupts actin filaments in astrocytes and fibroblasts. 1994 John Wiley & Sons, Inc. 相似文献
85.
RGDS肽对大鼠主动脉球囊内膜剥脱后血管壁增殖的影响 总被引:1,自引:0,他引:1
在大鼠主动脉球囊内膜剥脱术后血管壁细胞过度增殖模型上,用合成的血小板膜纤维蛋白原受体(glycoproteinⅡb/Ⅲacomplex,GPⅡb/Ⅲa)拮抗剂RGDS(Arg-Gly-Asp-Ser,50μmol·kg-1·d-1)治疗可有效地抑制损伤血管壁的细胞计数增加和内膜增厚以及血管平滑肌细胞增殖,显著降低其血管组织3H-TdR和3H-Leu的参入增加程度。实验结果提示RGDS肽作为血管成型术的辅佐剂,对于防治血管再狭窄可能具有潜在的临床应用前景。 相似文献
86.
研究了天然杀伤(NK)细胞对受致死剂量γ线照射的同系小鼠的造血调控作用。AMS/5小鼠经9Gyγ线全身照射后立即经尾静脉注射NK细胞(5×105),可明显提高受照小鼠30d活存率,照后8d小鼠骨髓中CFU-GM数量明显高于对照和脾细胞注射组,照射后30d,NK细胞注射组活存小鼠的骨髓有核细胞数和CFU-GM数已恢复到正常的76%─96%。病理组织学观察显示,输注NK细胞可使小鼠骨髓、脾脏的组织损伤程度减轻,造血功能增强,表现为造血灶数增多,造血细胞功能活跃,核分裂相增多,且涉及红系、粒系、巨核细胞系造血。NK细胞可能通过直接与造血干细胞相互作用或改善造血微环境等促进“内源性”造血功能,从而发挥对造血的正调控作用。提示NK细胞在小鼠造血功能的平衡维持中起重要作用。 相似文献
87.
本实验观察了活性钙调素(CaM)含量和CaM依赖性蛋白激酶Ⅱ(CaMkinaseⅡ)活性在急性低氧(模拟海拔7000m,5h)和常氧对照大鼠脑子组织中的变化。用流式产胞仪(FACS)所测两组动物脑皮层细胞的CaM,平均荧光强度分别为40.0±4.9和46.1±5.8,急性低氧组明显低于常氧对照组(P<0.05);用同位素液闪计数法所测两组动物皮层脑匀浆提取液中CaMkinaseⅡ活性,分别为184.3±8.1和198.8±9.4pmolPi·min-1·mg-1pro,急性低氧组明显低于常氧对照组(P<0.01)。结果提示CaM和CaMkinaseⅡ对低氧较为敏感,急性低氧时中枢神经细胞结构或功能的紊乱可能与活性CaM的含量减少和CaMkinaseⅡ活性能下降有关。 相似文献
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90.
工业固体废弃物的急性生物毒性研究 总被引:7,自引:0,他引:7
工业固体废弃物的急性生物毒性研究谢思琴,顾宗濂,周德智(中国科学院南京土壤研究所,南京210008)Acutebiotoxicityofindustrialsolidwastes¥.XieSiqin;GuZonglianandZhouDezhi(In... 相似文献