Dysregulation of exosomal miR-192 and miR-194 expression in lung adenocarcinoma patients

Non-small cell lung cancer (NSCLC) is the main reason of cancer linked mortality and around 80% of cases diagnosed in advanced stage. Therefore current study designed to evaluate the deregulation of miRNA-194 and miRNA-192 in different body fluid of Non small cell lung cancer participants. Present study recruited newly diagnosed histopathologically confirmed. It was observed that the 40% NSCLC participants showed elevated miR-194 expression and 60% NSCLC participants showed reduced miR-194 expression in serum sample while in Bronchial wash, only 20% NSCLC participants showed elevated miR-194 expression while 80% showed reduced miR-194 expression (p = 0.003). It was found that the 54% NSCLC participants showed elevated miR-192 expression and 55% NSCLC participants showed reduced miR-192 expression in serum sample while In Bronchial wash sample, only 25% NSCLC participants showed high miR-192 expression while 75% showed low miR-192 expression (P = 0.0004). Expression of miR-194 was significantly associated with TNM stages (p < 0.0001, p < 0.0001), distant organ metastases (p < 0.0001, p < 0.0001), pathological grade (p = 0.0009, p = 0.0005) among serum sample and bronchial wash sample. Same observation was found with expression of miR-192 and it was significantly associated with TNM stages (p < 0.0001, p < 0.0001), distant organ metastases (p < 0.0001, p < 0.0001), pathological grade (p = 0.006, p = 0.001) among serum sample and bronchial wash sample. It was observed that the NSCLC participants who had high serum based miR-194 expression showed 22 months of overall median survival while low expression of serum based miR-194 expression showed 18 months of overall median survival. Present study suggests that decreased expression of miR-194 and miR-192 was significantly associated with different clinical features of NSCLC cases. However, significantly higher number of NSCLC cases showed low expression of miR-194 and miR-192 in bronchial lavage sample. Decreased poor overall survival was found to be associated with bronchial wash sample with respect to low miR-194 and miR-192 expression while NSCLC participants showed better overall survival with high miR-194 and miR-192 expression. This suggested decreased expression of miR-192 and miR-194 expression could be the potential prognostic marker among NSCLC participants.     

基于遥感信息的黄土高原主要灌溉农业分布区生态安全特征

生态安全是区域经济社会可持续发展的重要保证.本研究基于2000-2018年间的遥感数据,系统分析了黄土高原4个主要灌溉农业分布区(银川平原、河套平原、汾河谷地和渭河平原)植被覆盖度(FVC)和遥感生态指数(RSEI)的变化特征.结果 表明:2000-2018年间,研究区FVC整体呈下降趋势,但4个不同灌溉农业分布区FV...     

子宫内膜异位细胞改良体外培养技术的研究进展

体外细胞培养是子宫内膜异位性疾病研究的重要工具.本文回顾了细胞体外培养技术在分离、纯化环节的改良以及子宫内膜异位性疾病永生化细胞系改造中的研究进展,并总结了近几年来激素及细胞因子诱导培养、细胞共培养及三维培养技术在该疾病体外机制研究中的应用,并对其发展前景进行了展望.     

遗传学课程思政教育的探索与实践

课程思政对高校落实立德树人、实现人才培养目标具有重要的意义。在专业课中进行课程思政改革有助于探究高校专业课程中育人功能的发挥。遗传学是生命科学专业重要的基础课程,在生命科学知识体系的构建中具有重要作用。立足内蒙古农业大学示范课程改革的教学实践,我们重塑了教学目标,改革教学方法,挖掘遗传学课程中蕴含的思政元素,使德育教育深入教师心、学生心,贯穿于教学、教评全过程,使知识传授、能力培养与价值引领同向同行,以提升学生的综合素质。     

重组人BD3-BPI的内毒素中和活性及其在高盐环境中抗菌活性的稳定性

目的：验证重组人BD3-BPI（rhBD3-BPI）是否具有内毒素中和活性,研究其在高盐环境中是否能保持抗菌活性。方法：根据内毒素标准品绘制内毒素活性标准曲线,将100 μL梯度稀释的rhBD3-BPI-与100 μL 10EU／mL脂多糖（LPS）混匀,37℃水浴60min,同时设标准对照（只含10EU／mL LPS的标准品溶液）,并以无热源水作为空白对照,采用基质显色法进行鲎试验测定LPS的活性;将6×10^8 CFU／mL的革兰阳性和阴性标准菌株及临床分离的多药耐药菌株接种于含1mg／mL rhBD3-BPI和0～250mmol／L不同浓度NaCl的液体细菌培养基中,37℃培养3h后用10mmol／L磷酸钠按1：1～1：1000的比例稀释,铺LB培养基平板,37℃过夜培养,观察各平板菌落生长情况,计数并计算杀菌率。结果：在5EU／mL的标准内毒素体系中,当rhBD3-BPI的浓度高于4μg／mL时即开始表现出一定的内毒素中和活性,当rhBD3-BPI的浓度分别为16、32 μg／mL时,其内毒素中和率分别为23％和88％,随后rhBD3-BPI对内毒素的中和活性趋于平稳,50 μg／mL的rhBD3-BPI对所有受检菌均表现出100％的杀伤效应。当NaCl浓度低于150mmol／L时,rhBD3-BPI对各受检菌的杀菌活性均未受明显影响;NaCI浓度升高至150-200mmol/L,rhBD3-BPI对各受检菌的杀菌活性有所下降,但其杀伤率仍在90％以上;当NaCl浓度高于200mmol／L时,盐浓度对rhBD3-BPI杀菌活性的影响才较为明显,但即使NaCl浓度达到250mmol／L,rhBD3-BPI的杀菌活性仍保持在85％以上。结论：rh-BD3-BPI具有内毒素中和活性,在高盐环境中具有良好的抗菌活性稳定性。     

HIV Populations Are Large and Accumulate High Genetic Diversity in a Nonlinear Fashion

HIV infection is characterized by rapid and error-prone viral replication resulting in genetically diverse virus populations. The rate of accumulation of diversity and the mechanisms involved are under intense study to provide useful information to understand immune evasion and the development of drug resistance. To characterize the development of viral diversity after infection, we carried out an in-depth analysis of single genome sequences of HIV pro-pol to assess diversity and divergence and to estimate replicating population sizes in a group of treatment-naive HIV-infected individuals sampled at single (n = 22) or multiple, longitudinal (n = 11) time points. Analysis of single genome sequences revealed nonlinear accumulation of sequence diversity during the course of infection. Diversity accumulated in recently infected individuals at rates 30-fold higher than in patients with chronic infection. Accumulation of synonymous changes accounted for most of the diversity during chronic infection. Accumulation of diversity resulted in population shifts, but the rates of change were low relative to estimated replication cycle times, consistent with relatively large population sizes. Analysis of changes in allele frequencies revealed effective population sizes that are substantially higher than previous estimates of approximately 1,000 infectious particles/infected individual. Taken together, these observations indicate that HIV populations are large, diverse, and slow to change in chronic infection and that the emergence of new mutations, including drug resistance mutations, is governed by both selection forces and drift.     

Whole-Brain Functional Connectivity Identification of Functional Dyspepsia

Recent neuroimaging studies have shown local brain aberrations in functional dyspepsia (FD) patients, yet little attention has been paid to the whole-brain resting-state functional network abnormalities. The purpose of this study was to investigate whether FD disrupts the patterns of whole-brain networks and the abnormal functional connectivity could reflect the severity of the disease. The dysfunctional interactions between brain regions at rest were investigated in FD patients as compared with 40 age- and gender- matched healthy controls. Multivariate pattern analysis was used to evaluate the discriminative power of our results for classifying patients from controls. In our findings, the abnormal brain functional connections were mainly situated within or across the limbic/paralimbic system, the prefrontal cortex, the tempo-parietal areas and the visual cortex. About 96% of the subjects among the original dataset were correctly classified by a leave one-out cross-validation approach, and 88% accuracy was also validated in a replication dataset. The classification features were significantly associated with the patients’ dyspepsia symptoms, the self-rating depression scale and self-rating anxiety scale, but it was not correlated with duration of FD patients (p>0.05). Our results may indicate the effectiveness of the altered brain functional connections reflecting the disease pathophysiology underling FD. These dysfunctional connections may be the epiphenomena or causative agents of FD, which may be affected by clinical severity and its related emotional dimension of the disease rather than the clinical course.     

Single Cell Analysis of Lymph Node Tissue from HIV-1 Infected Patients Reveals that the Majority of CD4+ T-cells Contain One HIV-1 DNA Molecule

Genetic recombination contributes to the diversity of human immunodeficiency virus (HIV-1). Productive HIV-1 recombination is, however, dependent on both the number of HIV-1 genomes per infected cell and the genetic relationship between these viral genomes. A detailed analysis of the number of proviruses and their genetic relationship in infected cells isolated from peripheral blood and tissue compartments is therefore important for understanding HIV-1 recombination, genetic diversity and the dynamics of HIV-1 infection. To address these issues, we used a previously developed single-cell sequencing technique to quantify and genetically characterize individual HIV-1 DNA molecules from single cells in lymph node tissue and peripheral blood. Analysis of memory and naïve CD4⁺ T cells from paired lymph node and peripheral blood samples from five untreated chronically infected patients revealed that the majority of these HIV-1-infected cells (>90%) contain only one copy of HIV-1 DNA, implying a limited potential for productive recombination in virus produced by these cells in these two compartments. Phylogenetic analysis revealed genetic similarity of HIV-1 DNA in memory and naïve CD4⁺ T-cells from lymph node, peripheral blood and HIV-1 RNA from plasma, implying exchange of virus and/or infected cells between these compartments in untreated chronic infection.