全文获取类型
收费全文 | 11267篇 |
免费 | 946篇 |
国内免费 | 1631篇 |
专业分类
13844篇 |
出版年
2024年 | 53篇 |
2023年 | 218篇 |
2022年 | 481篇 |
2021年 | 705篇 |
2020年 | 528篇 |
2019年 | 629篇 |
2018年 | 509篇 |
2017年 | 398篇 |
2016年 | 526篇 |
2015年 | 747篇 |
2014年 | 889篇 |
2013年 | 888篇 |
2012年 | 1100篇 |
2011年 | 950篇 |
2010年 | 559篇 |
2009年 | 515篇 |
2008年 | 623篇 |
2007年 | 464篇 |
2006年 | 446篇 |
2005年 | 397篇 |
2004年 | 368篇 |
2003年 | 276篇 |
2002年 | 259篇 |
2001年 | 203篇 |
2000年 | 172篇 |
1999年 | 154篇 |
1998年 | 117篇 |
1997年 | 97篇 |
1996年 | 70篇 |
1995年 | 91篇 |
1994年 | 59篇 |
1993年 | 50篇 |
1992年 | 52篇 |
1991年 | 42篇 |
1990年 | 39篇 |
1989年 | 46篇 |
1988年 | 25篇 |
1987年 | 22篇 |
1986年 | 16篇 |
1985年 | 20篇 |
1984年 | 13篇 |
1983年 | 15篇 |
1982年 | 8篇 |
1981年 | 3篇 |
1950年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
31.
本文首次报道了五步蛇身体主要部位的数量性状与其排毒量的关系。其中我们发现与排毒量有关的部位是头积、体粗、体重、佛指长。 相似文献
32.
33.
J Paquette K Nicoghosian G R Qi N Beauchemin R Cedergren 《European journal of biochemistry》1990,189(2):259-265
Conformational analyses using the single-strand-specific nuclease from mung bean and restriction endonucleases have been performed on a series of DNA fragments related to the sequence of the yeast initiator tRNA(Met). Mung bean nuclease cleaves DNA fragments exclusively in some, but not all, single-stranded regions as predicted by RNA secondary structural rules. Comparison of cleavage patterns of yeast initiator tRNA(Met), tDNA(Met) (a DNA oligomer having the sequence of tRNA(Met] and the anti-tDNA(Met) (the complement of tDNA(Met] suggests that the conformation of the three molecules is very similar. Furthermore, both tDNA and anti-tDNA are cleaved by HhaI and CfoI restriction endonucleases at two GCG/C sites which would be in double-stranded regions (the acceptor and dihydrouridine stem), if the two molecules adopt the tRNA cloverleaf structure. On the other hand, minor cleavage products show that the core region, i.e. the extra loop area, is slightly more exposed in tDNA and in anti-tDNA than in tRNA. Therefore, we submit that the global conformation of nucleic acids is primarily dictated by the interaction of purine and pyrimidine bases with atoms and functional groups common to both RNA and DNA. In this view the 2'-hydroxyl group, in tRNA at least, is an auxiliary structural feature whose role is limited to fostering local interactions, which increase the stability of a given conformation. 相似文献
34.
35.
Cloning and sequencing of a human pancreatic tumor mucin cDNA 总被引:24,自引:0,他引:24
M S Lan S K Batra W N Qi R S Metzgar M A Hollingsworth 《The Journal of biological chemistry》1990,265(25):15294-15299
A monospecific polyclonal antiserum against deglycosylated human pancreatic tumor mucin was used to select human pancreatic mucin cDNA clones from a lambda gt11 cDNA expression library developed from a human pancreatic tumor cell line. The full-length 4.4-kilobase mucin cDNA sequence included a 72-base pair 5'-untranslated region and a 307-base pair 3'-untranslated region. The predicted amino acid sequence for this cDNA revealed a protein of 122,071 daltons containing 1,255 amino acid residues of which greater than 60% were serine, threonine, proline, alanine, and glycine. Approximately two-thirds of the protein sequence consisted of identical 20-amino acid tandem repeats which were flanked by degenerate tandem repeats and nontandem repeat sequences on both the amino-terminal and carboxyl-terminal ends. The amino acid sequence also contained five putative N-linked glycosylation sites, a putative signal sequence and transmembrane domain, and numerous serine and threonine residues (potential O-linked glycosylation sites) outside and within the tandem repeat position. The cDNA and deduced amino acid sequence of the pancreatic mucin sequence was over 99% homologous with a mucin cDNA sequence derived from breast tumor mucin, even though the native forms of these molecules are quite distinct in size and degree of glycosylation. 相似文献
36.
Shuai Ma Shuhui Sun Jiaming Li Yanling Fan Jing Qu Liang Sun Si Wang Yiyuan Zhang Shanshan Yang Zunpeng Liu Zeming Wu Sheng Zhang Qiaoran Wang Aihua Zheng Shuguang Duo Yang Yu Juan Carlos Izpisua Belmonte Piu Chan Qi Zhou Moshi Song Weiqi Zhang Guang-Hui Liu 《Cell research》2021,(4):415-432
Aging is a major risk factor for many diseases,especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Diseas... 相似文献
37.
38.
在辽宁沈阳农田生态系统国家野外科学观测研究站,利用运行2a的开顶式气室,研究了臭氧(O3)浓度升高和不同氮肥施用水平对土壤线虫群落的影响。结果表明:(1)O3浓度升高降低了成熟期小麦根生物量。O3浓度升高和不同氮肥施用水平的交互作用改变了小麦成熟期土壤微生物生物量碳、氮和水溶性有机碳的含量。低氮条件下,O3浓度升高降低了土壤微生物生物量碳、氮和水溶性有机碳的含量;而高氮条件下则表现出相反的趋势。(2)O3浓度升高和不同氮肥施用水平对土壤线虫总数没有产生显著影响,而在灌浆期,食细菌线虫和食真菌线虫中c-p值为4(Ba4 and Fu4)的功能团对O3浓度升高和不同氮肥施用水平的响应敏感;与对照相比,不同氮处理中,O3浓度升高均降低了灌浆期Ba4功能团线虫的数量。灌浆期,O3浓度升高条件下,与对照相比Fu4功能团线虫数量在高氮条件下表现出增加的趋势,而在低氮条件表现出降低的趋势。(3)O3浓度升高和不同氮肥施用水平的交互作用显著影响了小麦灌浆期线虫的成熟度指数(MI)和结构指数(SI)。与对照相比,线虫成熟度指数和结构指数在低氮条件下随O3浓度升高而降低;而在高氮条件下随O3浓度升高而升高。上述结果表明,氮肥的施用能够缓解O3浓度升高对土壤食物网的扰动。 相似文献
39.
Meng Xu Nan Chang Taotian Tu Jimin Sun Jinyong Jiang Yu Xia Wenge Tang Hengqing Ji Xiaotao Zhao Jin Zhu Li Qi Xiaobo Liu Qiyong Liu 《PLoS neglected tropical diseases》2022,16(5)
BackgroundDengue fever has been a significant public health challenge in China. This will be particularly important in the context of global warming, frequent international travels, and urbanization with increasing city size and population movement. In order to design relevant prevention and control strategies and allocate health resources reasonably, this study evaluated the economic burden of dengue fever in China in 2019.MethodsThe economic burden of dengue fever patients was calculated from both family and the organisation perspectives. A survey was conducted among 1,027 dengue fever patients in Zhejiang, Chongqing, and Yunnan Provinces. Treatment expenses, lost working days, and insurance reimbursement expenses information were collected to estimate the total economic burden of dengue fever patients in 2019. The expenditures related to dengue fever prevention and control from government, Center for Disease Control and Prevention (CDC), communities and subdistrict offices of 30 counties (or districts) in Zhejiang Province and Chongqing City were also collected.ResultsThe direct, indirect and total economic burden for dengue fever patients in 2019 in the three Provinces were about 36,927,380.00 Chinese Yuan (CNY), 10,579,572.00 CNY and 46,805,064.00 CNY, respectively. The costs for prevention and control of dengue fever for the counties (or districts) without cases, counties (or districts) with imported cases, and counties (or districts) with local cases are 205,800.00 CNY, 731,180.00 CNY and 6,934,378.00 CNY, respectively. The total investment of dengue fever prevention and control in the 30 counties in China in 2019 was approximately 3,166,660,240.00 CNY.ConclusionThe economic burden of dengue fever patients is relatively high, and medical insurance coverage should be increased to lighten patients’ direct medical economic burden. At the same time, the results suggests that China should increase funding for primary health service institutions to prevent dengue fever transmission. 相似文献
40.