湖羊瘤胃微生物GH9家族葡聚糖酶基因IDSGLUC9-25的表达与功能表征

【目的】葡聚糖酶是饲用添加剂的重要成分,本研究旨在从湖羊消化道微生物中挖掘性质优良的GH9家族葡聚糖酶基因,用于研发新型饲用酶制剂。【方法】从湖羊瘤胃微生物cDNA中扩增IDSGLUC9-25基因,在大肠杆菌中进行异源表达,对重组蛋白进行诱导表达和纯化,研究重组蛋白的酶学性质和底物水解模式。【结果】IDSGLUC9-25基因编码527个氨基酸,包含一个CelD_N结构和一个GH9家族催化结构域;重组蛋白rIDSGLUC9-25分子量约为62.7 kDa,最适反应温度和pH分别为40℃和6.0,在30-50℃下活性较高,在pH 4.0-8.0范围内能够保持较高的稳定性,经pH 4.0-8.0缓冲液处理1 h后残余活性均大于90%;底物谱分析表明,rIDSGLUC9-25能催化大麦β-葡聚糖、苔藓地衣多糖、魔芋胶和木葡聚糖,比活性分别为(443.55±24.48)、(65.56±5.98)、(122.37±2.85)和(159.16±7.73) U/mg;利用薄层色谱法(thin layer chromatography, TLC)和高效液相色谱法(high performance liquid chromatography, HPLC)分析水解产物发现,rIDSGLUC9-25降解大麦葡聚糖主要生成纤维三糖(占总还原糖64.19%±1.19%)和纤维四糖(占总还原糖26.24%±0.12%),催化地衣多糖主要生成纤维三糖(占总还原糖78.46%±0.89%)。【结论】本研究报道了一种来自密螺旋体属细菌的内切β-1,4-葡聚糖酶IDSGLUC9-25 (EC 3.2.1.4),能高效催化多糖底物生成纤维三糖和纤维四糖,为研发饲用酶制剂和制备低聚寡糖建立基础。     

Ecological and methodological drivers of non-stationarity in tree growth response to climate

Radial tree growth is sensitive to environmental conditions, making observed growth increments an important indicator of climate change effects on forest growth. However, unprecedented climate variability could lead to non-stationarity, that is, a decoupling of tree growth responses from climate over time, potentially inducing biases in climate reconstructions and forest growth projections. Little is known about whether and to what extent environmental conditions, species, and model type and resolution affect the occurrence and magnitude of non-stationarity. To systematically assess potential drivers of non-stationarity, we compiled tree-ring width chronologies of two conifer species, Picea abies and Pinus sylvestris, distributed across cold, dry, and mixed climates. We analyzed 147 sites across the Europe including the distribution margins of these species as well as moderate sites. We calibrated four numerical models (linear vs. non-linear, daily vs. monthly resolution) to simulate growth chronologies based on temperature and soil moisture data. Climate–growth models were tested in independent verification periods to quantify their non-stationarity, which was assessed based on bootstrapped transfer function stability tests. The degree of non-stationarity varied between species, site climatic conditions, and models. Chronologies of P. sylvestris showed stronger non-stationarity compared with Picea abies stands with a high degree of stationarity. Sites with mixed climatic signals were most affected by non-stationarity compared with sites sampled at cold and dry species distribution margins. Moreover, linear models with daily resolution exhibited greater non-stationarity compared with monthly-resolved non-linear models. We conclude that non-stationarity in climate–growth responses is a multifactorial phenomenon driven by the interaction of site climatic conditions, tree species, and methodological features of the modeling approach. Given the existence of multiple drivers and the frequent occurrence of non-stationarity, we recommend that temporal non-stationarity rather than stationarity should be considered as the baseline model of climate–growth response for temperate forests.     

艾滋病病毒蛋白酶高效表达载体及体外活性检测系统的构建

艾滋病是本世纪80年代初发现的一种烈性传染病,5年病死率为100％,致病因子为人免疫缺陷病毒,该病毒的蛋白酶在病毒复制和成熟中具有决定性的意义。由于目前国内外尚未获得艾滋病病毒蛋白酶的高效表达的重组子及活性检测系统,限制了它的研究与应用。本文利用PCR技术修饰了艾滋病病毒蛋白酶的基因,使其具有便于克隆及表达用的限制酶切位点及转录终止码,井在其C末端设置了一个可用于检验该酶活性的特殊序列。DNA序列分析揭示上述突变策略成功,将修饰后的艾滋病病毒蛋白酶基因克隆入大肠杆菌表达系统,并获得高效表达(>30％),Western-Bolt鉴定结果表明所表达的蛋白为艾滋病病毒所特有,并具有较好的生物活性。     

Decomposing trends in bird populations: Climate,life histories and habitat affect different aspects of population change

Aim

Despite the complexity of population dynamics, most studies concerning current changes in bird populations reduce the trajectory of population change to a linear trend. This may hide more complex patterns reflecting responses of bird populations to changing anthropogenic pressures. Here, we address this complexity by means of multivariate analysis and attribute different components of bird population dynamics to different potential drivers.

Location

Czech Republic.

Methods

We used data on population trajectories (1982–2019) of 111 common breeding bird species, decomposed them into independent components by means of the principal component analysis (PCA), and related these components to multiple potential drivers comprising climate, land use change and species' life histories.

Results

The first two ordination axes explained substantial proportion of variability of population dynamics (42.0 and 12.5% of variation in PC1 and PC2 respectively). The first axis captured linear population trend. Species with increasing populations were characterized mostly by long lifespan and warmer climatic niches. The effect of habitat was less pronounced but still significant, with negative trends being typical for farmland birds, while positive trends characterized birds of deciduous forests. The second axis captured the contrast between hump-shaped and U-shaped population trajectories and was even more strongly associated with species traits. Species migrating longer distances and species with narrower temperature niches revealed hump-shaped population trends, so that their populations mostly increased before 2000 and then declined. These patterns are supported by the trends of total abundances of respective ecological groups.

Main Conclusion

Although habitat transformation apparently drives population trajectories in some species groups, climate change and associated species traits represent crucial drivers of complex population dynamics of central European birds. Decomposing population dynamics into separate components brings unique insights into non-trivial patterns of population change and their drivers, and may potentially indicate changes in the regime of anthropogenic effects on biodiversity.     

Annotated chromosome numbers of selected asiaticPotentilla species

Chromosome numbers are reported for 19 collections representing 16 AsiaticPotentilla taxa. The first chromosome records are reported forP. desertorum Bunge var.arnavatensis Wolf (2n=28),P. festiva Soják (2n=28),P. griffithii Hook f. subsp.beauvaisii (Cardot) Soják (2n=42),P. micropetala D. Don subsp.byssitecta (Soják) Měsí?ek etSoják (2n=14),P. mollissima Lehm. (2n=28),P. moorcroftii Wall. exLehm. (2n=42),P. multicaulis Bunge (2n=14),P. [x]omissa Soják (2n=35, 56, 70) andP. stanjukoviczii Ovcz. exKoczk. (2n=14). Counts differing from those previously recorded are given forP. algida Soják (2n=56) andP. flagellaris Willd. exSchlecht. (2n=42). Chromosome numbers of the following species were confirmed:P. [x]agrimonioides Bieb. (2n=42),P. chinensis Ser. in DC. (2n=14),P. fragarioides L. (2n=14),P. lineata Trev. (2n=14) andP. sericea L. (2n=28). Taxonomy is briefly discussed. A new combinationP. micropetala D. Don subsp.byssitecta (Soják) Měsí?ek etSoják stat. nov. is proposed.     

The complex genome and adaptive evolution of polyploid Chinese pepper (Zanthoxylum armatum and Zanthoxylum bungeanum)

Zanthoxylum armatum and Zanthoxylum bungeanum, known as ‘Chinese pepper’, are distinguished by their extraordinary complex genomes, phenotypic innovation of adaptive evolution and species-special metabolites. Here, we report reference-grade genomes of Z. armatum and Z. bungeanum. Using high coverage sequence data and comprehensive assembly strategies, we derived 66 pseudochromosomes comprising 33 homologous phased groups of two subgenomes, including autotetraploid Z. armatum. The genomic rearrangements and two whole-genome duplications created large (~4.5 Gb) complex genomes with a high ratio of repetitive sequences (>82%) and high chromosome number (2n = 4x = 132). Further analysis of the high-quality genomes shed lights on the genomic basis of involutional reproduction, allomones biosynthesis and adaptive evolution in Chinese pepper, revealing a high consistent relationship between genomic evolution, environmental factors and phenotypic innovation. Our study provides genomic resources and new insights for investigating diversification and phenotypic innovation in Chinese pepper, with broader implications for the protection of plants under severe environmental changes.     

黄河流域植被格局变化对水分利用效率的影响

黄河流域横跨3个气候带,是全球人类活动最为强烈的地区之一,特殊的地理位置和复杂的下垫面导致其碳-水循环过程较为复杂。研究黄河流域碳水循环不仅是区域水资源利用的基础,也是实现气候变化条件下双碳目标的关键。水分利用效率(WUE)作为表征碳水过程的重要指标,可用于反映生态系统碳水耦合规律及其相互作用关系。基于此,利用全球陆表特征参量数据(GLASS)的净初级生产力(NPP)和蒸散(ET)产品以及中国逐年土地利用与覆盖数据集(CLUD-A),分析了黄河流域植被格局变化背景下WUE在1990—2018年的时空变化特征及其驱动力。结果表明：(1)黄河流域全域WUE在29 a的均值处在0.18—1.53 g C/kg H₂O之间,存在明显的空间异质性,上游地区WUE明显高于中下游地区,分别在0.66—0.92 g C/kg H₂O和0.43—0.62 g C/kg H₂O之间波动,二者均存在波动上升态势。(2)黄河流域全域WUE在以2000年为中间点的10 a的增速达到近29 a的峰值,流域植被格局变化所带来的流域内NPP与ET变化速...     

基于地表水与地下水分割校正的漓江流域水供给服务时空格局研究

喀斯特区域水循环过程与其他地区存在显著区别,其水供给服务也具有独特的空间分布特征。然而,目前针对喀斯特地区产水量的时空变化研究较少考虑喀斯特生态系统的特殊性,导致产水量的空间模拟结果存在一定偏差。根据喀斯特地貌形态和地下水补径排泄特征提出了一种产水量分割方法,并基于水量平衡原理建立了地表水与地下水分割校正概念模型。选取喀斯特山区典型区域漓江流域为研究区,通过上述方法校正了InVEST模型产水量模拟结果,从栅格、地貌分区和子流域3个尺度分析了漓江流域2000—2020年产水量校正前与校正后时空分布格局。研究结果表明：(1)漓江流域2000—2020年InVEST模型产水量呈现先减后增再减的趋势,水供给服务空间分布格局为北高南低。产水深度在岩溶区和非岩溶区几乎没有差异,但在岩溶区内部差异明显：裸露型岩溶区产水深度高于覆盖型和埋藏型,峰林平原产水深度高于峰丛洼地。子流域分区平均产水深度排序为漓江上游区>漓江中游区>恭城河区>荔浦河区>漓江下游区。(2)利用地表与地下水分割校正后,水供给服务空间分布格局为四周高于中部,西北部高于东南部。非岩溶区产水深度明显高于岩溶区,埋...