Recovery from ethyl methanesulphonate-induced genetical damage in barley. Independence of sodium azide treatment

Barley seeds were treated for 3 h at 25°C with 240 mM ethyl methanesulphonate (EMS), washed for 18 h, treated with various concentrations of unbuffered sodium azide (pH 6.7–7.3) for 3 h at 25°C, re-dried to 30% water content and either sown immediately or stored at 25°C for 12 days and then sown. The synergistic action of sodium azide post-treatment has been demonstrated only for the EMS-induced M₁ germination reduction, while the EMS-induced M₁ sterility and the yield of M₂ chlorophyll mutants were unaffected. The ?storage” recovery from EMS-induced mutagenic effects was insensitive to sodium azide post-treatment. The 12 day-seed storage at 25°C brought about an improvement of M₁ germination, M₁ survival, M₁ fertility and a decrease in the amount of M₂ mutants, regardless of whether sodium azide post-treatment was applied or not.     

A contribution to the phenotype distribution of phosphoglucomutase in Czechoslovakia (the district of České Budějovice)

Summary A population sample of 416 unrelated donors from eské Budjovice (southorn Bohemia) was investigated for the phenotypes of phosphoglucomutase (PGM). The calculated frequencies of the allelles PGM ₁ ¹ and PGM ₁ ² , 0.770 and 0.230, respectively, correspond to the expected frequencies of the phenotypes PGM 1=0.593, PGM 2-1=0.354, and PGM 2=0.0529. No rare phonotype was detected.     

Galium procurrens,a new diploid relic species of theG. sylvaticum-group from the Balkan Peninsula

Galium procurrens is described as a new diploid relic species from Montenegro/N. Albania and SW. Bulgaria. It is related to the tetraploidG. laevigatum and other diploid and polyploid taxa of theG. sylvaticum-group inhabiting European deciduous forests.     

Studien über dieJungermannioideae (Hepaticae) 8.Jungermannia subg.Plectocolea und subg.Solenostoma in Australien,Neuseeland und Ozeanien

Die achte Studie über die SubfamilieJungermannioideae (Jungermanniaceae, Hepaticae) ist dén Arten der GattungJungermannia L. emend.Dum., die in Australien, Neuseeland und Ozeanien vorkommen, gewidmet. Die meist endemischen Arten, die auf Hawaii vorkommen, werden als eine selbständige (neunte) Studie bearbeitet. Im genannten Gebiet wurden 13 Arten der GattungJungermannia L. emend.Dum. festgestellt.J. (P.) hasskarliana (Nees) Steph.,J. (P.) obliquifolia (Schiffn.) Váňa,J. (P.) tetragona Lindenb.,J. (P.) hirticalyx Steph.,J. (P.) minutiverrucosa Amak.,J. (P.) wattsiana Steph.,J. (S.) ariadne Tayl.,J. (S.) totipapillosa Hodgs.,J. (S.) inundata Hook. fil. etTayl. emend.Mitt. undJ. (S.) orbiculata (Col.) Grolle sind eingehend taxonomisch bearbeitet,J. (P.) micrantha (Mitt.) Steph. wird in die nächste Studie eingereiht.J. (P.) boninensis (Horik.) Inoue wurde vonInoue (Inoue etIwatsuki 1969) eingehend beschrieben und abgebildet,Haplozia comptonii Pears. wurde schon im 2. Teil diskutiert. Die anderen aus dem Gebiet bekannten Arten sind mit den obenerwähnten Arten synonymisiert oder zu anderen Gattungen gestellt.     

Oxidative stress-resistance assay for screening yeast strains overproducing heterologous proteins

Many natural proteins have been developed into drugs and produced for direct application. Identifying improved hosts to achieve high-level heterologous protein production is a challenge in the study of heterologous protein expression in recombinant yeast. In this study, a novel high-throughput assay to screen such overproducing Saccharomyces cerevisiae strains was systematically developed. The protocol designed was based on screening host strain derivatives with increased superoxide dismutase dependent resistance to oxidative stress. Yeast cells transformed with recombinant plasmid carrying SOD1 gene as a reporter responded exquisitely to oxidative stress induced by elevated concentrations of paraquat. Improved yeast strains resulting from screening clones subjected to genome shuffling through selective pressure argue for a more effective screening system compared with traditonal selection. Moreover, this approach can be employed in general biochemical analysis without utilization of flow cytometry or well plate reader. Therefore, it is expected that the high-throughput assay would make superior strains producing heterologous proteins.