全文获取类型
收费全文 | 1570篇 |
免费 | 155篇 |
国内免费 | 2篇 |
出版年
2023年 | 12篇 |
2022年 | 35篇 |
2021年 | 68篇 |
2020年 | 31篇 |
2019年 | 35篇 |
2018年 | 51篇 |
2017年 | 43篇 |
2016年 | 58篇 |
2015年 | 94篇 |
2014年 | 106篇 |
2013年 | 112篇 |
2012年 | 152篇 |
2011年 | 141篇 |
2010年 | 89篇 |
2009年 | 76篇 |
2008年 | 82篇 |
2007年 | 80篇 |
2006年 | 70篇 |
2005年 | 68篇 |
2004年 | 52篇 |
2003年 | 40篇 |
2002年 | 43篇 |
2001年 | 7篇 |
2000年 | 3篇 |
1999年 | 5篇 |
1998年 | 13篇 |
1997年 | 9篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 6篇 |
1988年 | 6篇 |
1987年 | 6篇 |
1985年 | 3篇 |
1984年 | 5篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1976年 | 6篇 |
1974年 | 4篇 |
1973年 | 9篇 |
1972年 | 4篇 |
1965年 | 3篇 |
1964年 | 5篇 |
1961年 | 4篇 |
1960年 | 6篇 |
1902年 | 2篇 |
1899年 | 2篇 |
排序方式: 共有1727条查询结果,搜索用时 31 毫秒
101.
102.
Paraoxonases are associated with intestinal inflammatory diseases and intracellularly localized to the endoplasmic reticulum 总被引:2,自引:0,他引:2
Rothem L Hartman C Dahan A Lachter J Eliakim R Shamir R 《Free radical biology & medicine》2007,43(5):730-739
We demonstrated previously that the paraoxonase (PON1/2/3) genes and proteins are expressed in human intestinal biopsies and in Caco-2 cells. The current study aims were to explore whether PON1/2/3 expression is different in inflammatory bowel diseases (IBD) or celiac disease compared to healthy controls, and to explore the intracellular localization of PON1/2/3. Our results showed that significantly fewer biopsies expressed PON1 and PON3 in the duodenum of celiac patients (PON1, P<0.0001; PON3, P=0.03), in the terminal ileum of Crohn's patients (PON1, P=0.001; PON3, P=0.008), and in the colon of UC patients (PON1, P=0.02; PON3, P=0.06) compared to controls. Since all three disorders share markedly elevated inflammatory mediators we explored the PON1/2/3 mRNA expression on cytokine stimulation. No changes were observed in Caco-2 and HT29 cells. Immunofluorescence experiments localized PON1/2/3 exclusively to the endoplasmic reticulum (ER) in both CaCo-2 and HT29 cells. These results demonstrate for the first time a novel relationship between PON1 and PON3 expression and several inflammatory gastrointestinal disorders. Together with the localization of PON1/2/3 enzymes to the ER, it may be suggested that PON1/2/3 may have extracellular functions as part of the host response in IBD and celiac disease. 相似文献
103.
104.
tRNA‐dependent alanylation of diacylglycerol and phosphatidylglycerol in Corynebacterium glutamicum 下载免费PDF全文
Angela M. Smith Jesse S. Harrison Christopher D. Grube Austin E. F. Sheppe Nahoko Sahara Ryohei Ishii Osamu Nureki Hervé Roy 《Molecular microbiology》2015,98(4):681-693
Aminoacyl‐phosphatidylglycerol synthases (aaPGSs) are membrane proteins that utilize aminoacylated tRNAs to modify membrane lipids with amino acids. Aminoacylation of membrane lipids alters the biochemical properties of the cytoplasmic membrane and enables bacteria to adapt to changes in environmental conditions. aaPGSs utilize alanine, lysine and arginine as modifying amino acids, and the primary lipid recipients have heretofore been defined as phosphatidylglycerol (PG) and cardiolipin. Here we identify a new pathway for lipid aminoacylation, conserved in many Actinobacteria, which results in formation of Ala‐PG and a novel alanylated lipid, Alanyl‐diacylglycerol (Ala‐DAG). Ala‐DAG formation in Corynebacterium glutamicum is dependent on the activity of an aaPGS homolog, whereas formation of Ala‐PG requires the same enzyme acting in concert with a putative esterase encoded upstream. The presence of alanylated lipids is sufficient to enhance the bacterial fitness of C. glutamicum cultured in the presence of certain antimicrobial agents, and elucidation of this system expands the known repertoire of membrane lipids acting as substrates for amino acid modification in bacterial cells. 相似文献
105.
Symbiodinium transcriptome and global responses of cells to immediate changes in light intensity when grown under autotrophic or mixotrophic conditions 下载免费PDF全文
106.
107.
108.
Ting Wang Robert Grabski Elizabeth Sztul Jesse C. Hay 《Traffic (Copenhagen, Denmark)》2015,16(2):148-171
Tethering factors regulate the targeting of membrane‐enclosed vesicles under the control of Rab GTPases. p115, a golgin family tether, has been shown to participate in multiple stages of ER/Golgi transport. Despite extensive study, the mechanism of action of p115 is poorly understood. SNARE proteins make up the machinery for membrane fusion, and strong evidence shows that function of p115 is directly linked to its interaction with SNAREs. Using a gel filtration binding assay, we have demonstrated that in solution p115 stably interacts with ER/Golgi SNAREs rbet1 and sec22b, but not membrin and syntaxin 5. These binding preferences stemmed from selectivity of p115 for monomeric SNARE motifs as opposed to SNARE oligomers. Soluble monomeric rbet1 can compete off p115 from coat protein II (COPII) vesicles. Furthermore, excess p115 inhibits p115 function in trafficking. We conclude that monomeric SNAREs are a major binding site for p115 on COPII vesicles, and that p115 dissociates from its SNARE partners upon SNAREpin assembly. Our results suggest a model in which p115 forms a mixed p115/SNARE helix bundle with a monomeric SNARE, facilitates the binding activity and/or concentration of the SNARE at prefusion sites and is subsequently ejected as SNARE complex formation and fusion proceed. 相似文献
109.
Jarrod A Chapman Martin Mascher Ayd?n Bulu? Kerrie Barry Evangelos Georganas Adam Session Veronika Strnadova Jerry Jenkins Sunish Sehgal Leonid Oliker Jeremy Schmutz Katherine A Yelick Uwe Scholz Robbie Waugh Jesse A Poland Gary J Muehlbauer Nils Stein Daniel S Rokhsar 《Genome biology》2015,16(1)
Polyploid species have long been thought to be recalcitrant to whole-genome assembly. By combining high-throughput sequencing, recent developments in parallel computing, and genetic mapping, we derive, de novo, a sequence assembly representing 9.1 Gbp of the highly repetitive 16 Gbp genome of hexaploid wheat, Triticum aestivum, and assign 7.1 Gb of this assembly to chromosomal locations. The genome representation and accuracy of our assembly is comparable or even exceeds that of a chromosome-by-chromosome shotgun assembly. Our assembly and mapping strategy uses only short read sequencing technology and is applicable to any species where it is possible to construct a mapping population.
Electronic supplementary material
The online version of this article (doi:10.1186/s13059-015-0582-8) contains supplementary material, which is available to authorized users. 相似文献110.
Genqiao Li Ying Wang Ming-Shun Chen Erena Edae Jesse Poland Edward Akhunov Shiaoman Chao Guihua Bai Brett F Carver Liuling Yan 《BMC genomics》2015,16(1)