全文获取类型
收费全文 | 1665篇 |
免费 | 61篇 |
国内免费 | 1篇 |
出版年
2023年 | 4篇 |
2022年 | 7篇 |
2021年 | 9篇 |
2020年 | 10篇 |
2019年 | 13篇 |
2018年 | 21篇 |
2017年 | 15篇 |
2016年 | 42篇 |
2015年 | 66篇 |
2014年 | 56篇 |
2013年 | 100篇 |
2012年 | 85篇 |
2011年 | 120篇 |
2010年 | 69篇 |
2009年 | 55篇 |
2008年 | 100篇 |
2007年 | 112篇 |
2006年 | 114篇 |
2005年 | 97篇 |
2004年 | 79篇 |
2003年 | 112篇 |
2002年 | 89篇 |
2001年 | 13篇 |
2000年 | 14篇 |
1999年 | 20篇 |
1998年 | 23篇 |
1997年 | 11篇 |
1996年 | 13篇 |
1995年 | 21篇 |
1994年 | 17篇 |
1993年 | 13篇 |
1992年 | 15篇 |
1991年 | 19篇 |
1990年 | 15篇 |
1989年 | 12篇 |
1988年 | 4篇 |
1987年 | 10篇 |
1986年 | 14篇 |
1985年 | 9篇 |
1984年 | 12篇 |
1983年 | 7篇 |
1982年 | 8篇 |
1981年 | 9篇 |
1980年 | 10篇 |
1978年 | 8篇 |
1977年 | 10篇 |
1976年 | 4篇 |
1975年 | 5篇 |
1970年 | 4篇 |
1965年 | 3篇 |
排序方式: 共有1727条查询结果,搜索用时 15 毫秒
21.
Rules incorporating influences on global temperature, an estimate of radiation balance, were induced from astronomical, geophysical, and anthropogenic variables. During periods of intermediate global temperatures (generally like the present century), the influences assume cancelingroles; influences cancel the effects of extreme states potentially imposed by other influences because they are, in aggregate, most likely to be assuming opposite values. This imparts an overall stability to the global temperature. To achieve cold or hot global temperature, influences assume reinforcingroles. CO
2 is an active influence on global temperature. By virtue of its constancy in the atmosphere, it can be expected to sponsor frequent hot years in combination with the other influences as they cycle through their periods. If measures were implemented to maintain warm or cool global temperatures, it could retain the status quoof present global agricultural regions. They are probably more productive than hot world regions would be because of narrow storm tracks. 相似文献
22.
Abstract: Histamine (HA) potently stimulated cyclic AMP accumulation in intact pineal glands taken from light-exposed chicks. The action of HA was stronger in the presence of forskolin and the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX). The effect of HA was mimicked by HA H1- and H2-receptor-selective agonists in the following order of potency: HA > 4-methylhistamine (H2) > 2-methylhistamine (H1) > 2-thiazolylethylamine (H1) ≫ dimaprit (H2). The HA H3-receptor-selective agonist (R)α-methylhistamine was poorly active. The effect of HA was antagonized by selective H2-receptor blockers (tiotidine > oxmetidine > cimetidine = ranitidine) and was not significantly affected by the selective H1- and H3-receptor blockers mepyramine and thioperamide. A detailed analysis of an antagonistic action of ranitidine (versus HA) revealed a noncompetitive mode of action of the H2 blocker. The stimulatory action of the H1 agonist 2-thiazolylethylamine (both under basal conditions and in the presence of forskolin or IBMX) was not significantly influenced by three H1-receptor-selective blockers (mepyramine, triprolidine, and diphenhydramine), but it was totally counteracted by ranitidine. Using accepted selective agonists and antagonists of the HA H1, H2, and H3 receptor we were unable to identify clearly the receptor subtype mediating the HA action on the cyclic AMP-generating system of the chick pineal. It is suggested that the receptor under consideration may represent either an H2-like (in terms of mammalian criteria) or avian-specific HA receptor. The data suggest that HA may be considered a modulator of the pineal activity in chicks. 相似文献
23.
Zagrodzki Paweł Mietelski Jerzy W. Krośniak Mirosław Petelenz Barbara 《Biological trace element research》1994,(1):273-277
The aim of this work was to check whether the stable cesium content in forest litter affects the value of radiocesium from
litter-to-mushroom transfer factorTf or not. Total cesium in litter, measured by AAS, varied from 0.1–2.7 μg/g. These data, combined with earlier results for
mushrooms, showed no simple correlation forTf. More complex relationships provided very high correlation coefficients, but their validity needs further investigation. 相似文献
24.
The precision of extrachromosomal homologous recombination and gene targeting in plant cells was investigated. Recombination was directed to introns of selectable marker genes where potential changes could persist without affecting the function and therefore the selectability of the genes. Approximately 9 kb of crossover regions was rescued and sequenced. Changes were detected at a frequency below one point mutation per 1000 bp, indicating that extrachromosomal recombination and gene targeting both appear to occur with high fidelity. 相似文献
25.
A crude nuclear fraction of resting wheat embryos was used as the source of putative plant minichromosomes: unique DNA sequences
the size of genes and flanked by telomere-type repeats. Preliminary separation of low-molecular-weight DNA species from chromosomal
DNA (Hirt's method), velocity sedimentation, and isopycnic centrifugation were followed by PCR amplification of minichromosome-like
sequences. The most abundant PCR product was cloned and sequenced. In addition to telomeric repeats (defined by a PCR primer),
which were the expected sequences, the linear DNA molecule (637 pb) contained an ARS-like element, RAP1-binding site, and
two relatively long ORFs. The whole sequence seems to represent a naturally occurring plant minichromosome. 相似文献
26.
Ana J. Coito Maria de Sousa Jerzy W. Kupiec-Weglinski 《Cell communication & adhesion》1994,2(3):249-255
The specific adhesion of cells to other cells or to particular tissue microenvirorvments is a basic function of cell migration and recognition, and underlines many biologic processes including embryogenesis, repair and immunity. Leukocytes express an array of surface receptors broadly known as “accessory adhesion molecules.” which mediate most cell -cell interactions, direct lymphocyte traffic between anatomical compartments, and facilitate cellular adhesion to the inflammation or alloantigenic sites (Springer 1990). In addition, adhesion molecules are involved in the process of antigen recognition, and may costimulate cell activation and transformation. These proteins are thought to affect the very early antigen independent events between host leukocytes and vascular endothelium. Because of these activities, the subject of adhesion molecules is gaining interest in the field of organ transplantation, in both conceptualization and development of novel therapeutic strategies (de Sousa et al. 1991, Kupiec-Weglinski et al. 1993a, Heemann et al. 1993). 相似文献
27.
Kay Huebner Teresa Druck Sal LaForgia Jerzy Lasota Carlo M. Croce Luisa Lanfrancone Emilio Donti Gina Pengue Girolama La Mantia Pier-Giuseppe Pelicci Luigi Lania 《Human genetics》1993,91(3):217-222
cDNA clones encoding zinc finger motifs were isolated by screening human placenta and T-cell (Peer) cDNA libraries with zinc finger (ZNF) consensus sequences. Unique cDNA clones were mapped in the human genome by rodent-human somatic cell hybrid analysis and in some cases in situ chromosomal hybridization. ZNF 80 mapped to 3p12-3qter, ZNF 7 was previously mapped to 8q24 and is here shown by in situ hybridization and use of appropriate hybrids to map telomeric to the MYC locus. ZNF 79 mapped to 9q34 centromeric to the ABL gene and between a constitutional chromosomal translocation on the centromeric side and the CML specific ABL translocation on the telomeric side. ZNF77 mapped to 19p while ZNF 78L1 (pT3) mapped to 19q. Chromosome 19 carries many ZNF loci and other genes with zinc finger encoding motifs; the pT3 clone additionally detected a locus designated ZNF 78L2, which mapped to chromosome region 1p, most likely in the region 1p32 where the MYCL and JUN loci map. 相似文献
28.
Summary DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences
in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands
than those obtained with probe 33.15. Assay of a regenerative somaclonal variant (F49R) by DNA fingerprinting with probe 33.6
detected mutation that was unlinked to the regenerative trait. The fingerprints obtained under the applied conditions also
demonstrated genetic stability of consecutive generations of the regenerants in tissue culture. DNA fingerprints of F1 plants revealed that each polymorphic band was inherited from either one or the other parent. Both probes distinguished individual-specific
genotypes in seven cultivars of red clover. Greater variability in DNA fingerprints was detected between (V=0.899) than within
(0.417≤V≤0.548) cultivars. 相似文献
29.
Phytochemistry Reviews - Sea buckthorn (Elaeagnus rhamnoides; syn. Hippophae rhamnoides) is a thorny shrub or a small tree belonging to the Elaeagnaceae family, native to Eurasia. Sea buckthorn... 相似文献
30.
Jerzy K. Kulski Silvana Gaudieri Matthew Bellgard Lois Balmer Keith Giles Hidetoshi Inoko Roger L. Dawkins 《Journal of molecular evolution》1997,45(6):599-609
Sequence analysis of a 237 kb genomic fragment from the central region of the MHC has revealed that the HLA-B and HLA-C genes
are contained within duplicated segments peri-B (53 kb) and peri-C (48 kb), respectively, and separated by an intervening
sequence (IF) of 30 kb. The peri-B and peri-C segments share at least 90% sequence homology except when interrupted by insertions/deletions
including Alu, L1, an endogenous retrovirus, and pseudogenes. The sequences of peri-B, IF, and peri-C were searched for the
presence of Alu elements to use as markers of evolution, chromosomal rearrangements, and polymorphism. Of 29 Alu elements,
14 were identified in peri-B, 11 in peri-C, and 4 in IF. The Alu elements in peri-B and peri-C clustered phylogenetically
into two clades which were classified as ``preduplication' and ``postduplication' clades. Four Alu J elements that are shared
by peri-B and peri-C and are flanked by homologous sequences in their paralogous locations, respectively, clustered into a
``preduplication' clade. By contrast, the majority of Alu elements, which are unique to either peri-B or peri-C, clustered
into a postduplication clade together with the Alu consensus subfamily members ranging from platyrrhine-specific (Spqxcg)
to catarrhine-specific Alu sequences (Y). The insertion of platyrrhine-specific Alu elements in postduplication locations
of peri-B and peri-C implies that these two segments are the products of a duplication which occurred in primates prior to
the divergence of the New World primate from the human lineage (35–44 mya). Examination of the paralogous Alu integration
sites revealed that 9 of 14 postduplication Alu sequences have produced microsatellites of different length and sequence within
the Alu 3′-poly A tail. The present analysis supports the hypothesis that HLA-B and HLA-C genes are products of an extended
segmental duplication between 44 and 81 million years ago (mya), and that subsequent diversification of both genomic segments
occurred because of the mobility and mutation of retroelements such as Alu repeats.
Received: 21 May 1997 / Accepted: 9 July 1997 相似文献