全文获取类型
收费全文 | 153篇 |
免费 | 22篇 |
出版年
2022年 | 2篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2018年 | 1篇 |
2016年 | 2篇 |
2015年 | 16篇 |
2014年 | 10篇 |
2013年 | 5篇 |
2012年 | 11篇 |
2011年 | 8篇 |
2010年 | 6篇 |
2009年 | 6篇 |
2008年 | 6篇 |
2007年 | 8篇 |
2006年 | 4篇 |
2005年 | 9篇 |
2004年 | 8篇 |
2003年 | 8篇 |
2002年 | 8篇 |
2001年 | 8篇 |
2000年 | 6篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1984年 | 2篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1969年 | 2篇 |
1968年 | 1篇 |
排序方式: 共有175条查询结果,搜索用时 31 毫秒
91.
Haiyi Lou Yan Lu Dongsheng Lu Ruiqing Fu Xiaoji Wang Qidi Feng Sijie Wu Yajun Yang Shilin Li Longli Kang Yaqun Guan Boon-Peng Hoh Yeun-Jun Chung Li Jin Bing Su Shuhua Xu 《American journal of human genetics》2015,96(1):54-69
Because of past limitations in samples and genotyping technologies, important questions about the history of the present-day Greenlandic population remain unanswered. In an effort to answer these questions and in general investigate the genetic history of the Greenlandic population, we analyzed ∼200,000 SNPs from more than 10% of the adult Greenlandic population (n = 4,674). We found that recent gene flow from Europe has had a substantial impact on the population: more than 80% of the Greenlanders have some European ancestry (on average ∼25% of their genome). However, we also found that the amount of recent European gene flow varies across Greenland and is far smaller in the more historically isolated areas in the north and east and in the small villages in the south. Furthermore, we found that there is substantial population structure in the Inuit genetic component of the Greenlanders and that individuals from the east, west, and north can be distinguished from each other. Moreover, the genetic differences in the Inuit ancestry are consistent with a single colonization wave of the island from north to west to south to east. Although it has been speculated that there has been historical admixture between the Norse Vikings who lived in Greenland for a limited period ∼600–1,000 years ago and the Inuit, we found no evidence supporting this hypothesis. Similarly, we found no evidence supporting a previously hypothesized admixture event between the Inuit in East Greenland and the Dorset people, who lived in Greenland before the Inuit. 相似文献
92.
While social media has become an important platform for social reputation, the emotional responses of users toward bad news have not been investigated thoroughly. We analyzed a total of 20,773 Twitter messages by 15,513 users to assess the influence of bad news and public apology in social media. Based on both computerized, quantitative sentiment analysis and in-depth qualitative analysis, we found that rapid public apology effectively and immediately reduced the level of negative sentiment, where the degree of change in sentiments differed by the type of interactions users engaged in. The majority of users who directly conversed with corporate representatives on the new media were not typical consumers, but experts and practitioners. We extend the existing cognitive model and suggest the audiences’ psychological reaction model to describe the information processing process during and after an organizational crisis and response. We also discuss various measures through which companies can respond to a crisis properly in social media in a fashion that is different from conventional mass media. 相似文献
93.
Chee Sian Kuan Su Mei Yew Yue Fen Toh Chai Ling Chan Soo Kun Lim Kok Wei Lee Shiang Ling Na Chee-Choong Hoh Wai-Yan Yee Kee Peng Ng 《PloS one》2015,10(12)
Peritonitis is the leading complication of peritoneal dialysis, which is primarily caused by bacteria rather than fungi. Peritonitis is responsible for approximately 18% of the infection-related mortality in peritoneal dialysis patients. In this paper, we report the isolation of a rare fungus, Quambalaria cyanescens, from the peritoneal fluid of a man after he switched from continuous ambulatory peritoneal dialysis to nocturnal intermittent peritoneal dialysis. Based on the morphological examination and multigene phylogeny, the clinical isolate was confirmed as Q. cyanescens. This pathogen exhibited low sensitivity to all tested echinocandins and 5-flucytosine. Interestingly, morphological characterization revealed that Q. cyanescens UM 1095 produced different pigments at low temperatures (25°C and 30°C) on various culture media. It is important to monitor the emergence of this rare fungus as a potential human pathogen in the tropics. This study provides insight into Q. cyanescens UM 1095 phenotype profiles using a Biolog phenotypic microarray (PM). Of the 760 nutrient sources tested, Q. cyanescens UM 1095 utilized 42 compounds, and the fungus can adapt to a broad range of osmotic and acidic environments. To our knowledge, this is the first report of the isolation of Q. cyanescens from peritoneal fluid, revealing this rare fungus as a potential human pathogen that may be misidentified using conventional methods. The detailed morphological, molecular and phenotypic characterization of Q. cyanescens UM 1095 provides the basis for future studies on its biology, lifestyle, and potential pathogenicity. 相似文献
94.
Christopher J. Millow Susan A. Mackintosh Rebecca L. Lewison Nathan G. Dodder Eunha Hoh 《PloS one》2015,10(5)
Persistent organic pollutants (POPs) are typically monitored via targeted mass spectrometry, which potentially identifies only a fraction of the contaminants actually present in environmental samples. With new anthropogenic compounds continuously introduced to the environment, novel and proactive approaches that provide a comprehensive alternative to targeted methods are needed in order to more completely characterize the diversity of known and unknown compounds likely to cause adverse effects. Nontargeted mass spectrometry attempts to extensively screen for compounds, providing a feasible approach for identifying contaminants that warrant future monitoring. We employed a nontargeted analytical method using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC/TOF-MS) to characterize halogenated organic compounds (HOCs) in California Black skimmer (Rynchops niger) eggs. Our study identified 111 HOCs; 84 of these compounds were regularly detected via targeted approaches, while 27 were classified as typically unmonitored or unknown. Typically unmonitored compounds of note in bird eggs included tris(4-chlorophenyl)methane (TCPM), tris(4-chlorophenyl)methanol (TCPMOH), triclosan, permethrin, heptachloro-1''-methyl-1,2''-bipyrrole (MBP), as well as four halogenated unknown compounds that could not be identified through database searching or the literature. The presence of these compounds in Black skimmer eggs suggests they are persistent, bioaccumulative, potentially biomagnifying, and maternally transferring. Our results highlight the utility and importance of employing nontargeted analytical tools to assess true contaminant burdens in organisms, as well as to demonstrate the value in using environmental sentinels to proactively identify novel contaminants. 相似文献
95.
I Komuro Y Katoh T Kaida Y Shibazaki M Kurabayashi E Hoh F Takaku Y Yazaki 《The Journal of biological chemistry》1991,266(2):1265-1268
96.
97.
Lucia H.D. Kang Agita Rughani Matthew L. Walker Rosa Bestak Joseph F.Y. Hoh 《The journal of histochemistry and cytochemistry》2010,58(7):623-634
We test the hypothesis that cat jaw satellite cells belong to a distinct lineage preprogrammed to express masticatory-specific isoforms of myosin heavy-chain (m-MyHC), myosin-binding protein-C (m-MBP-C), and tropomyosin (m-Tm) during myogenesis in vitro. A monoclonal antibody (MAb) against m-MyHC and MAbs raised here against cat m-MBP-C and m-Tm were used to stain cryostat sections of cat masseter muscle and cultured myotubes derived from satellite cells of cat temporalis and limb muscles, using peroxidase immunohistochemistry. MAbs against m-MBP-C bound purified m-MBP-C in Western blots. MAbs against m-Tm failed to react with m-Tm in Western blots, but reacted with native m-Tm in gel electrophoresis–derived ELISA. In cat masseter sections, MAbs against m-MyHC, m-MBP-C, and m-Tm stained all masticatory fibers, but not the jaw-slow fibers. Cat jaw and limb muscle cultures mature significantly more slowly relative to rodent cultures. However, at 3 weeks, all three MAbs extensively stained temporalis myotubes, whereas they apparently stained isolated myotubes weakly in cat limb and rat jaw cultures. We conclude that satellite cells of masticatory fibers are preprogrammed to express these isoforms during myogenesis in vitro. These results consolidate the notion that masticatory and limb muscle allotypes are distinct. (J Histochem Cytochem 58:623–634, 2010) 相似文献
98.
Stretching cardiac myocytes stimulates protooncogene expression 总被引:29,自引:0,他引:29
I Komuro T Kaida Y Shibazaki M Kurabayashi Y Katoh E Hoh F Takaku Y Yazaki 《The Journal of biological chemistry》1990,265(7):3595-3598
99.
During bacterial chemotaxis, a cell acquires information about its environment by sampling changes in the local concentration
of a chemoattractant, and then uses that information to bias its motion relative to the source of the chemoattractant. The
trajectory of a chemotaxing bacteria is thus a spatial manifestation of the information gathered by the cell. Here we show
that a recently developed approach for computing spatial information using Fourier coefficient probabilities, the k-space
information (kSI), can be used to quantify the information in such trajectories. The kSI is shown to capture expected responses
to gradients of a chemoattractant. We then extend the k-space approach by developing an experimental probability distribution
(EPD) that is computed from chemotactic trajectories collected under a reference condition. The EPD accounts for connectivity
and other constraints that the nature of the trajectories imposes on the k-space computation. The EPD is used to compute the
spatial information from any trajectory of interest, relative to the reference condition. The EPD-based spatial information
also captures the expected responses to gradients of a chemoattractant, although the results differ in significant ways from
the original kSI computation. In addition, the entropy calculated from the EPD provides a useful measure of trajectory space.
The methods developed are highly general, and can be applied to a wide range of other trajectory types as well as non-trajectory
data. 相似文献
100.
Charline Bacchus-Souffan Mark Fitch Jori Symons Mohamed Abdel-Mohsen Daniel B. Reeves Rebecca Hoh Mars Stone Joseph Hiatt Peggy Kim Abha Chopra Haelee Ahn Vanessa A. York Daniel L. Cameron Frederick M. Hecht Jeffrey N. Martin Steven A. Yukl Simon Mallal Paul U. Cameron Steven G. Deeks Joshua T. Schiffer Sharon R. Lewin Marc K. Hellerstein Joseph M. McCune Peter W. Hunt 《PLoS pathogens》2021,17(1)
The precise role of CD4 T cell turnover in maintaining HIV persistence during antiretroviral therapy (ART) has not yet been well characterized. In resting CD4 T cell subpopulations from 24 HIV-infected ART-suppressed and 6 HIV-uninfected individuals, we directly measured cellular turnover by heavy water labeling, HIV reservoir size by integrated HIV-DNA (intDNA) and cell-associated HIV-RNA (caRNA), and HIV reservoir clonality by proviral integration site sequencing. Compared to HIV-negatives, ART-suppressed individuals had similar fractional replacement rates in all subpopulations, but lower absolute proliferation rates of all subpopulations other than effector memory (TEM) cells, and lower plasma IL-7 levels (p = 0.0004). Median CD4 T cell half-lives decreased with cell differentiation from naïve to TEM cells (3 years to 3 months, p<0.001). TEM had the fastest replacement rates, were most highly enriched for intDNA and caRNA, and contained the most clonal proviral expansion. Clonal proviruses detected in less mature subpopulations were more expanded in TEM, suggesting that they were maintained through cell differentiation. Earlier ART initiation was associated with lower levels of intDNA, caRNA and fractional replacement rates. In conclusion, circulating integrated HIV proviruses appear to be maintained both by slow turnover of immature CD4 subpopulations, and by clonal expansion as well as cell differentiation into effector cells with faster replacement rates. 相似文献