全文获取类型
收费全文 | 31177篇 |
免费 | 2468篇 |
国内免费 | 16篇 |
出版年
2022年 | 225篇 |
2021年 | 554篇 |
2020年 | 349篇 |
2019年 | 450篇 |
2018年 | 709篇 |
2017年 | 583篇 |
2016年 | 954篇 |
2015年 | 1529篇 |
2014年 | 1671篇 |
2013年 | 1906篇 |
2012年 | 2460篇 |
2011年 | 2305篇 |
2010年 | 1480篇 |
2009年 | 1268篇 |
2008年 | 1831篇 |
2007年 | 1647篇 |
2006年 | 1459篇 |
2005年 | 1382篇 |
2004年 | 1324篇 |
2003年 | 1132篇 |
2002年 | 1119篇 |
2001年 | 900篇 |
2000年 | 873篇 |
1999年 | 656篇 |
1998年 | 287篇 |
1997年 | 228篇 |
1996年 | 195篇 |
1995年 | 172篇 |
1994年 | 142篇 |
1993年 | 140篇 |
1992年 | 301篇 |
1991年 | 245篇 |
1990年 | 193篇 |
1989年 | 223篇 |
1988年 | 178篇 |
1987年 | 162篇 |
1986年 | 173篇 |
1985年 | 146篇 |
1984年 | 112篇 |
1983年 | 113篇 |
1982年 | 94篇 |
1980年 | 81篇 |
1979年 | 85篇 |
1978年 | 79篇 |
1975年 | 91篇 |
1974年 | 87篇 |
1973年 | 96篇 |
1972年 | 91篇 |
1970年 | 85篇 |
1969年 | 81篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
Theoretical models for cooperative steady-state ATPase activity of myosin subfragment-1 on regulated actin 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《Biophysical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Recent theoretical work on the cooperative equilibrium binding of myosin subfragment-1-ADP to regulated actin, as influenced by Ca2+, is extended here to the cooperative steady-state ATPase activity of myosin subfragment-1 on regulated actin. Exact solution of the general steady-state problem will require Monte Carlo calculations. Three interrelated special cases are discussed in some detail and sample computer (not Monte Carlo) solutions are given. The eventual objective is to apply these considerations to in vitro experimental data and to in vivo muscle models. 相似文献
42.
Willoughby de Broke Rudyard Kipling Hugh Elliot E. Ray Lankester Leonard Hill Laurence R. Philipps Wm. Arbuthnot Lane James Crichton-Browne H. Bryan Donkin Francis Lloyd R. A. Lyster John MacAlister F. W. Mott William Osler C. W. Saleeby J. H. Sequeira Humphry Rolleston Hugh Wansey Bayly 《BMJ (Clinical research ed.)》1919,2(3074):725
43.
Meal prepared from unheated rapeseed (Brassica napus cv. Zephyr) showed the presence of t,iocyanate ion, while meal from heated seed of the same cultivar did not show detectable amounts. Unheated seed meal on autolysis, and heated seed meal on incubation with thioglucosidase, yielded increased amounts of thiocyanate ion. Various commercial rapeseed meals showed the presence of t,iocyanate ion only after enzyme incubation. Low glucosinolate, cv. Bronowski, and higher glucosinolate, cv. Zephyr on enzymic incubation yielded comparable amounts of thiocyanate ion, suggesting that the precursor responsible in the two varieties was the same and present in similar quantities. No formation of thiocyanate ion was observed on incubation of sinigrin with thioglucosidase. Rats dosed with heated meal, containing intact glucosinolate, showed a slight increase of thiocyanate ion in the urine as compared with control rats dosed with water, while a relatively large increase followed dosing with sinigrin. Rats dosed with meal containing free thiocyanate ion excreted the ingested thiocyanate ion almost quantitatively. 相似文献
44.
45.
46.
47.
48.
49.
50.
Secretion of Recombinant Pediocin PA-1 by Bifidobacterium longum, Using the Signal Sequence for Bifidobacterial α-Amylase
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Gi-Seong Moon Yu-Ryang Pyun Myeong Soo Park Geun Eog Ji Wang June Kim 《Applied microbiology》2005,71(9):5630-5632
A recombinant DNA, encoding the chimeric protein of the signal sequence for bifidobacterial α-amylase mature pediocin PA-1, was introduced into Bifidobacterium longum MG1. Biologically active pediocin PA-1 was successfully secreted from the strain and showed bactericidal activity against Listeria monocytogenes and the same molecular mass as native pediocin PA-1. 相似文献