Morphological variability in response to palaeoenvironmental change – a case study on Cretaceous ammonites

Changes in ammonite morphology during the earliest Early Aptian (Cretaceous) of an epicontinental sea in northern Germany were investigated based on new and rich material of Deshayesites (Deshayesitidae). This is a globally distributed genus and one of the most important ammonites of the Cretaceous with respect to biostratigraphy and abundance. The purpose of our study was to describe changes in morphology over few hundred thousand years and to discuss their relationships to major palaeoenvironmental perturbations. Our material is derived from four different horizons, and the studied stratigraphical interval includes a sea‐level change, a period of warming and an oxygen depletion event. We observe variable patterns in the occurrence of different morphological groups through time, probably indicating that morphotypes were individually impacted by environmental change. These morphological groups, unequivocally attributed to Deshayesites, cannot be fit into the existing species classification system. The greatest morphological disparity in Deshayesites is multi‐causal. It may be the result of: (1) an invasion of two morphogroups new to the habitat, which thus immediately face considerably greater competition in an environment just recovered from low‐oxygen conditions in parts of the water column during an early interval within Oceanic Anoxic Event 1a (OAE 1a); or, (2) it may be due to a rise in sea level with a simultaneous invasion of competitors of Tethyan origin. This sheds new light on the current concept for high morphological variability within ammonite species and poses challenges to our current ideas about ammonite diversity and the use of well‐established index‐species in supraregional correlations.     

The global regulator LaeA controls production of citric acid and endoglucanases in <Emphasis Type="Italic">Aspergillus carbonarius</Emphasis>

The global regulatory protein LaeA is known for regulating the production of many kinds of secondary metabolites in Aspergillus species, as well as sexual and asexual reproduction, and morphology. In Aspergillus carbonarius, it has been shown that LaeA regulates production of ochratoxin. We have investigated the regulatory effect of LaeA on production of citric acid and cellulolytic enzymes in A. carbonarius. Two types of A. carbonarius strains, having laeA knocked out or overexpressed, were constructed and tested in fermentation. The knockout of laeA significantly decreased the production of citric acid and endoglucanases, but did not reduce the production of beta-glucosidases or xylanases. The citric acid accumulation was reduced with 74–96 % compared to the wild type. The endoglucanase activity was reduced with 51–78 %. Overexpression of LaeA seemed not to have an effect on citric acid production or on cellulose or xylanase activity.     

The membrane-induced structure of melittin is correlated with the fluidity of the lipids

The effect of the bee toxin melittin on DMPC dynamics in fast-tumbling bicelles has been investigated. The (13)C R(1) and (13)C-(1)H NOE relaxation parameters for DMPC were used to monitor the effect of melittin and cholesterol on lipid dynamics. It was found that melittin has the largest effect on the DMPC mobility in DMPC/DHPC bicelles, while less effect was observed in cholesterol-doped bicelles, or in bicelles made with CHAPS, indicating that the rigidity of the membrane affects the melittin-membrane interaction. CD spectra were analysed in terms of cooperativity of the alpha-helix to random coil transition in melittin, and these results also indicated similar differences between the bicelles. The study shows that bicelles can be used to investigate lipid dynamics by spin relaxation, and in particular of peptide-induced changes in membrane fluidity.     

Emerging roles of PPARdelta in metabolism

PPARdelta differs from the other two PPAR isotypes (alpha and gamma) by its more wide-spread tissue-specific expression pattern, its involvement in developmental processes and its profound impact on muscle and heart fat metabolism. Activation of PPARdelta modulates inflammatory responses of macrophages and is linked to altered lipoprotein metabolism, most importantly a significant raise of HDL cholesterol. PPARdelta activation in the liver decreases hepatic glucose output, thereby contributing to improved glucose tolerance and insulin sensitivity. Several studies have shown that PPARdelta polymorphisms are associated with plasma lipid levels, body mass index and the risk for diabetes and coronary heart disease. These findings support that high affinity PPARdelta agonists may be promising drugs of the future to treat the metabolic syndrome which is an expanding overweight-related health threat characterized by insulin resistance, hyperglycemia, dyslipidemia, hypertension, and accelerated atherosclerosis.