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101.
Spatial and temporal heterogeneity in the environment is a common feature affecting many natural populations. For example,
both the resource levels and optimal habitat choices of individuals likely change over time. One way for organisms to cope
with environmental variation is to display adaptive plasticity in traits such as behavior and morphology. Since trait plasticity
is hypothesized to be a prerequisite for character divergence, studies of mechanisms behind such plasticity are warranted.
In this study, we looked at the interaction of two potentially important environmental variables on behavioral and morphological
plasticity in Eurasian perch (Perca fluviatilis L.). More specifically, the plastic responses in activity and morphology of perch exposed to different resource levels and
simulated habitat types were studied in an aquarium experiment. The resource level experienced had a large influence on plasticity
in both activity and morphology. Behavioral adaptations have been thought to mediate morphological transitions, and we suggest
that the morphological response to the resource level was mediated by differences in activity and growth rates. The habitat
type also affected morphological plasticity but to a lesser extent, and there was no effect on activity from habitat type.
Based on these results, we suggest that it is essential to include several environmental factors acting in concert when studying
mechanisms behind trait plasticity. We also propose that variation in resource levels might play a key role in fostering trait
plasticity in at least fish populations, while other environmental variables such as divergent habitat complexities and prey
types might be less influential. Dynamics in resource levels and optimal habitat choices might thus be important factors influencing
character divergence in natural populations. 相似文献
102.
Wang C Li S Januschke J Rossi F Izumi Y Garcia-Alvarez G Gwee SS Soon SB Sidhu HK Yu F Matsuzaki F Gonzalez C Wang H 《Developmental cell》2011,21(3):520-533
Drosophila neural stem cells, larval brain neuroblasts (NBs), align their mitotic spindles along the apical/basal axis during asymmetric cell division (ACD) to maintain the balance of self-renewal and differentiation. Here, we identified a protein complex composed of the tumor suppressor anastral spindle 2 (Ana2), a dynein light-chain protein Cut up (Ctp), and Mushroom body defect (Mud), which regulates mitotic spindle orientation. We isolated two ana2 alleles that displayed spindle misorientation and NB overgrowth phenotypes in larval brains. The centriolar protein Ana2 anchors Ctp to centrioles during ACD. The centriolar localization of Ctp is important for spindle orientation. Ana2 and Ctp localize Mud to the centrosomes and cell cortex and facilitate/maintain the association of Mud with Pins at the apical cortex. Our findings reveal that the centrosomal proteins Ana2 and Ctp regulate Mud function to?orient the mitotic spindle during NB asymmetric division. 相似文献
103.
MAPK signalling is a complex process not only requiring the core components Raf, MEK and Erk, but also many proteins like the scaffold protein KSR and several kinases to specifically localize, modulate and fine-tune the outcome of the pathway in a cell context specific manner. In mammals, protein kinase CK2 was shown to bind to the scaffold protein KSR and to phosphorylate Raf proteins at a conserved serine residue in the negative-charge regulatory (N−) region, thereby facilitating maximal activity of the MAPK signalling pathway. In this work we show that in Drosophila CK2 is also bound to KSR. However, despite the presence of a corresponding serine residue in the N-region of DRaf, CK2-mediated phosphorylation of DRaf takes place on a serine residue at the N-terminus and is required for Erk activation. Previous work identified polyamines as regulators of CK2 kinase activity. The main cellular source of polyamines is the catabolism of amino acids. Evidence is provided that phosphorylation of DRaf by CK2 is modulated by polyamines, with spermine being the most potent inhibitor of the reaction. We suggest that CK2 is able to monitor intracellular polyamine levels and translates this information to modulate MAPK signalling. 相似文献
104.
105.
Krause JC Tsibane T Tumpey TM Huffman CJ Briney BS Smith SA Basler CF Crowe JE 《Journal of immunology (Baltimore, Md. : 1950)》2011,187(7):3704-3711
We generated from a single blood sample five independent human mAbs that recognized the Sa antigenic site on the head of influenza hemagglutinin and exhibited inhibitory activity against a broad panel of H1N1 strains. All five Abs used the V(H)3-7 and J(H)6 gene segments, but at least four independent clones were identified by junctional analysis. High-throughput sequence analysis of circulating B cells revealed that each of the independent clones were members of complex phylogenetic lineages that had diversified widely using a pattern of progressive diversification through somatic mutation. Unexpectedly, B cells encoding multiple diverging lineages of these clones, including many containing very few mutations in the Ab genes, persisted in the circulation. Conversely, we noted frequent instances of amino acid sequence convergence in the Ag combining sites exhibited by members of independent clones, suggesting a strong selection for optimal binding sites. We suggest that maintenance in circulation of a wide diversity of somatic variants of dominant clones may facilitate recognition of drift variant virus epitopes that occur in rapidly mutating virus Ags, such as influenza hemagglutinin. In fact, these Ab clones recognize an epitope that acquired three glycosylation sites mediating escape from previously isolated human Abs. 相似文献
106.
Richter S Neundorf I Loebner K Gräber M Berg T Bergmann R Steinbach J Pietzsch J Wuest F 《Bioorganic & medicinal chemistry letters》2011,21(16):4686-4689
Human polo-like kinase 1 (Plk1) is involved in cell proliferation and overexpressed in a broad variety of different cancer types. Due to its crucial role in cancerogenesis Plk1 is a potential target for diagnostic and therapeutic applications. Peptidic ligands can specifically interact with the polo-box domain (PBD) of Plk1, a C-terminal located phosphoepitope binding motif. Recently, phosphopeptide MQSpTPL has been identified as ligand with high binding affinity. However, a radiolabeled version of this peptide showed only insufficient cellular uptake. The present study investigated peptide dimers consisting of PBD-targeting phosphopeptide MQSpTPL and a cell-penetrating peptide (CPP) moiety. The new constructs demonstrate superior uptake in different cancer cell-lines compared to the phosphopeptide alone. Furthermore, we could demonstrate binding of phosphopeptide-CPP dimers to PBD of Plk1 making the compounds interesting leads for the development of molecular probes for imaging Plk1 in cancer. 相似文献
107.
Jens G. P. Diller Sophia Drescher Mario Hofmann Max Rabus Heike Feldhaar Christian Laforsch 《Ecology and evolution》2022,12(4)
Invasive alien species are a major threat to ecosystems. Invasive terrestrial plants can produce allelochemicals which suppress native terrestrial biodiversity. However, it is not known if leached allelochemicals from invasive plants growing in riparian zones, such as Impatiens glandulifera, also affect freshwater ecosystems. We used mesocosms and laboratory experiments to test the impact of I. glandulifera on a simplified freshwater food web. Our mesocosm experiments show that leachate from I. glandulifera significantly reduced population growth rate of the water flea Daphnia magna and the green alga Acutodesmus obliquus, both keystone species of lakes and ponds. Laboratory experiments using the main allelochemical released by I. glandulifera, 2‐methoxy‐1,4‐naphthoquinone, revealed negative fitness effects in D. magna and A. obliquus. Our findings show that allelochemicals from I. glandulifera not only reduce biodiversity in terrestrial habitats but also pose a threat to freshwater ecosystems, highlighting the necessity to incorporate cross‐ecosystem effects in the risk assessment of invasive species. 相似文献
108.
Summary The solubility of oxygen in the liquid phase of a bioreactor was changed by a ramp change of temperature, and kLa was determined from the resulting return to equilibrium of dissolved oxygen activity. The maximum kLa that can be measured by this method in a standard laboratory scale bioreactor is 145 h–1 corresponding to a temperature change rate of 320°C h–1.Nomenclature p
Difference between pG and pL (% saturation)
- T
Ramp change of temperature (°C)
- E
Temperature-compensated output from the oxygen electrode (A)
- Eu
Uncompensated output from the oxygen electrode (A)
- kLa
Overall volumetric mass transfer coefficient (h–1)
- kLaTm
Overall volumetric mass transfer coefficient at temperature Tm (h–1)
- PG
Dissolved oxygen activity in equilibrium with the gas phase (% saturation)
- pL
Dissolved oxygen activity (% saturation)
- pLm
Dissolved oxygen activity at time tm (% saturation)
- t
Time (h)
- tm
Time of maximum p (h)
- T
Temperature (°C)
- Tcal
Calibration temperature of the oxygen electrode (°C)
- Tm
Final temperature after a temperature shift (°C)
- Tn
Temperature at time tn 相似文献
109.
Stefania Testoni Elena Bartolone Marco Rossi Andrea Patrignani Rémy Bruggmann Peter Lichtner Jens Tetens Arcangelo Gentile Cord Dr?gemüller 《PloS one》2012,7(9)
In the last decade breeders of Romagnola cattle observed an outbreak of a new congenital anomaly. This lethal multi-organ developmental dysplasia is mainly characterized by facial deformities, ascites and hepatic fibrosis. Affected stillborn calves were inbred to a single founder sire suggesting autosomal monogenic recessive inheritance. We localized the causative mutation to a 1.2 Mb interval on BTA 17 by genome-wide association and identical by descent mapping. A solution-based method for targeted DNA capture combined with massively parallel sequencing was used to analyze the entire critical region containing 24 genes. Homozygosity for two non-synonymous coding sequence variants affecting the RNF34 and KDM2B genes was detected by evaluating one affected calf. Here we show that the disease phenotype is associated with a KDM2B missense mutation (c.2503G>A) leading to an amino acid exchange (p.D835N) in an evolutionary strongly conserved domain. In addition, the genetic makeup of three inbred cattle strongly supports the causality of the KDM2B mutation. This report of a naturally-occurring spontaneous mutation of a JmjC domain containing histone demethylase gene provides evidence for their important role in the endo- and mesodermal organ development. 相似文献
110.
Moritz Kneipp Jake Turner Sebastian Hambauer Sandro M. Krieg Jens Lehmberg Ute Lindauer Daniel Razansky 《PloS one》2014,9(4)