全文获取类型
收费全文 | 29722篇 |
免费 | 2511篇 |
国内免费 | 11篇 |
专业分类
32244篇 |
出版年
2023年 | 122篇 |
2022年 | 293篇 |
2021年 | 580篇 |
2020年 | 314篇 |
2019年 | 432篇 |
2018年 | 572篇 |
2017年 | 503篇 |
2016年 | 858篇 |
2015年 | 1440篇 |
2014年 | 1567篇 |
2013年 | 1897篇 |
2012年 | 2438篇 |
2011年 | 2385篇 |
2010年 | 1501篇 |
2009年 | 1310篇 |
2008年 | 1755篇 |
2007年 | 1805篇 |
2006年 | 1577篇 |
2005年 | 1554篇 |
2004年 | 1493篇 |
2003年 | 1392篇 |
2002年 | 1288篇 |
2001年 | 415篇 |
2000年 | 343篇 |
1999年 | 350篇 |
1998年 | 348篇 |
1997年 | 268篇 |
1996年 | 227篇 |
1995年 | 228篇 |
1994年 | 210篇 |
1993年 | 193篇 |
1992年 | 195篇 |
1991年 | 184篇 |
1990年 | 149篇 |
1989年 | 164篇 |
1988年 | 140篇 |
1987年 | 136篇 |
1986年 | 114篇 |
1985年 | 116篇 |
1984年 | 98篇 |
1983年 | 126篇 |
1982年 | 108篇 |
1981年 | 92篇 |
1980年 | 77篇 |
1979年 | 80篇 |
1978年 | 97篇 |
1977年 | 74篇 |
1976年 | 69篇 |
1974年 | 71篇 |
1973年 | 58篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
11.
12.
13.
Pierre Fellmann Jens Andersen Philippe F. Devaux Marc le Maire Alain Bienvenue 《Biochemical and biophysical research communications》1980,95(1):289-295
A spin labeled fatty acid (16-doxylstearic acid) was linked to a photochemical reacting group (azido derivative). When the molecule is introduced, at a low concentration, into rabbit sarcoplasmic reticulum membranes, the spectrum before illumination is identical to the spectrum obtained with the corresponding spin labeled fatty acid. After illumination, a large immobilized components is seen. It corresponds to about 70% of the ESR signal of the effectively bound label, at room temperature. The fraction of immobilized component varies with temperature, from 100% at 0°C to 50% at 35°C. Addition of a small amount of detergent (dodecyl octaethylene glycol monoether), under non solubilizing conditions, decreases the fraction of signal due to a strongly immobilized probe. A possible interpretation is that the immobilized signal reflects protein bound spin labels trapped in Ca2+ ATPase oligomers, which are partially dissociated by detergent addition or temperature increase. 相似文献
14.
Homozygous typing cells (HTC) were primed, using responding and stimulating lymphocytes of the same HLA-D groups. These intra-HLA-D group primings showed strong specific responses. Restimulation by HLA-D heterozygous and homozygous cell panels showed no correlation between the restimulating determinant and HLA-D. On the other hand, an unrelated individual, not carrying Dw4 and primed to Dw4 HTC, is restimulated by three of four Dw4-HTC. Thus, one non-HLA-D-associated restimulating determinant and another HLA-D-associated determinant could be identified. The differences among the four Dw4 HTC recognized in secondary MLC could reflect either recognition of separate gene products or recognition of separate determinants on the same gene product. 相似文献
15.
16.
17.
Ribonuclease III: new sense from nuisance. 总被引:6,自引:0,他引:6
Christian Conrad Reinhard Rauhut 《The international journal of biochemistry & cell biology》2002,34(2):116-129
RNases play an important role in the processing of precursor RNAs, creating the mature, functional RNAs. The ribonuclease III family currently is one of the most interesting families of endoribonucleases. Surprisingly, RNase III is involved in the maturation of almost every class of prokaryotic and eukaryotic RNA. We present an overview of the various substrates and their processing. RNase III contains one of the most prominent protein domains used in RNA-protein recognition, the double-stranded RNA binding domain (dsRBD). Progress in the understanding of this domain is summarized. Furthermore, RNase III only recently emerged as a key player in the new exciting biological field of RNA silencing, or RNA interference. The eukaryotic RNase III homologues which are likely involved in this process are compared with the other members of the RNase III family. 相似文献
18.
19.
Yanyan Han Elfriede Eppinger Ingrid G. Schuster Luise U. Weigand Xiaoling Liang Elisabeth Kremmer Christian Peschel Angela M. Krackhardt 《The Journal of biological chemistry》2009,284(48):33409-33417
The formin protein formin-like 1 (FMNL1) is highly restrictedly expressed in hematopoietic lineage-derived cells and has been previously identified as a tumor-associated antigen. However, function and regulation of FMNL1 are not well defined. We have identified a novel splice variant (FMNL1γ) containing an intron retention at the C terminus affecting the diaphanous autoinhibitory domain (DAD). FMNL1γ is specifically located at the cell membrane and cortex in diverse cell lines. Similar localization of FMNL1 was observed for a mutant lacking the DAD domain (FMNL1ΔDAD), indicating that deregulation of autoinhibition is effective in FMNL1γ. Expression of both FMNL1γ and FMNL1ΔDAD induces polarized nonapoptotic blebbing that is dependent on N-terminal myristoylation of FMNL1 but independent of Src and ROCK activity. Thus, our results describe N-myristoylation as a regulative mechanism of FMNL1 responsible for membrane trafficking potentially involved in a diversity of polarized processes of hematopoietic lineage-derived cells. 相似文献
20.