全文获取类型
收费全文 | 218626篇 |
免费 | 18047篇 |
国内免费 | 68篇 |
出版年
2018年 | 2363篇 |
2017年 | 2280篇 |
2016年 | 3114篇 |
2015年 | 3668篇 |
2014年 | 4485篇 |
2013年 | 6210篇 |
2012年 | 7249篇 |
2011年 | 7718篇 |
2010年 | 5394篇 |
2009年 | 4727篇 |
2008年 | 6616篇 |
2007年 | 6927篇 |
2006年 | 6530篇 |
2005年 | 6209篇 |
2004年 | 6409篇 |
2003年 | 6127篇 |
2002年 | 6009篇 |
2001年 | 9120篇 |
2000年 | 9265篇 |
1999年 | 7008篇 |
1998年 | 2432篇 |
1997年 | 2448篇 |
1996年 | 2200篇 |
1995年 | 2024篇 |
1993年 | 1989篇 |
1992年 | 5796篇 |
1991年 | 5873篇 |
1990年 | 5669篇 |
1989年 | 5625篇 |
1988年 | 5173篇 |
1987年 | 4923篇 |
1986年 | 4451篇 |
1985年 | 4617篇 |
1984年 | 3809篇 |
1983年 | 3231篇 |
1982年 | 2221篇 |
1981年 | 2002篇 |
1980年 | 1970篇 |
1979年 | 3532篇 |
1978年 | 2815篇 |
1977年 | 2558篇 |
1976年 | 2299篇 |
1975年 | 2784篇 |
1974年 | 3123篇 |
1973年 | 3008篇 |
1972年 | 2810篇 |
1971年 | 2595篇 |
1970年 | 2237篇 |
1969年 | 2150篇 |
1968年 | 1998篇 |
排序方式: 共有10000条查询结果,搜索用时 23 毫秒
931.
Limitations associated with immunoglobulins have motivated the search for novel binding scaffolds. Repeat proteins have emerged as one promising class of scaffolds, but often are limited to binding protein and peptide targets. An exception is the repeat proteins of the immune system, which have in recent years served as an inspiration for binding scaffolds which can bind glycans and other classes of biomolecule. Like other repeat proteins, these proteins can be very stable and have a monomeric mode of binding, with elongated and highly variable binding surfaces. The ability to target glycans and glycoproteins fill an important gap in current tools for research and biomedical applications. 相似文献
932.
933.
Journal of Ichthyology - The paper considers the effect of the Sea of Azov salinization on the food supply to anchovy Engraulis encrasicolus maeoticus as the level of salinity was rising from 12.7... 相似文献
934.
Relationships between the strongyloid nematodesRugopharynx delta, R. zeta, R. omega, R. longibursaris, R. mawsonae andR. sigma, all from macropodid marsupials, were investigated using allozyme data. The phylogenetic trees derived from the electrophoretic data set were congruent with those of the hosts and were consistent with the hypothesis that the species complex originated in pademelons of the genusThylogale and diversified in rock-wallabies (Petrogale spp.) and scrub wallabies of the subgenusNotamacropus. Host switching is evident only between closely related macropodid taxa. 相似文献
935.
936.
937.
938.
939.
940.
I C Zambrano A T Kowal L E Mortenson M W Adams M K Johnson 《The Journal of biological chemistry》1989,264(35):20974-20983
The two iron-only hydrogenases (I and II) from Clostridium pasteurianum have been investigated by variable temperature magnetic circular dichroism (MCD) and electron paramagnetic resonance (EPR) spectroscopies. Samples were studied both reduced with dithionite under an atmosphere of H2 and after oxidation with thionine. The results are consistent with four and two [4Fe-4S]1+,2+ (F)-clusters in hydrogenases I and II, respectively. All four F-clusters are reduced and paramagnetic in reduced hydrogenase I, with up to one exhibiting an S = 3/2 ground state and the remainder having conventional S = 1/2 ground states. Both F-clusters have S = 1/2 ground states in reduced hydrogenase II; however, one appears to be only partially reduced under the conditions used for reduction. MCD studies of the oxidized enzymes show no temperature-dependent features in the visible region which can be attributed to the EPR-active S = 1/2 hydrogen-activating cluster, suggesting predominantly oxygen and nitrogen coordination for the iron atoms of this center. However, temperature-dependent MCD transitions arising from a hitherto undetected S greater than 1/2 Fe-S clusters are apparent in both oxidized hydrogenases. Detailed EPR studies of oxidized hydrogenase I revealed resonances from an S = 3/2 species, however, spin quantitation reveals this to be a trace component that is unlikely to be responsible for the observed low temperature MCD spectrum. The nature and origin of these S greater than 1/2 Fe-S clusters are discussed in light of the available spectroscopic data for these and other iron-only hydrogenases. 相似文献