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91.
92.
Summary Asynchronous amoebal cultures of temperature-sensitive mutants of Physarum polycephalum were examined cytologically, and two cell cycle mutants were identified. Genetic analysis indicated that each mutant carried a single mutation that was expressed in both amoebal and plasmodial phases. Thus it is possible to isolate cell cycle mutations expressed in plasmodia by initial isolation and analysis of amoebal mutants, a quicker procedure than the alternative of isolating plasmodial mutants directly. The two mutants were studied further by measuring nuclear DNA contents and synthesis of macromolecules. Both mutants gave results consistent with a block in nuclear division. 相似文献
93.
94.
Jennifer Alvey 《American anthropologist》1998,100(3):824-824
Agrarian Reform and Class Consciousness in Nicaragua. Laura J. Enriquez. Gainesville: University Press of Florida, 1997.206 pp. 相似文献
95.
Patch assessment in foraging flocks of European starlings: evidence for the use of public information 总被引:8,自引:4,他引:4
A field experiment was carried out to determine whether group-foragingstarlings (Sturnus vulgaris) use public information to helpthem estimate the quality of an artificial resource patch anddepart accordingly. Three kinds of information are potentiallyavailable in a group: patch-sample information, pre-harvestinformation, and public information. These three types of informationcan be combined into four patch assessment strategies: (1) patch-samplealone; (2) patch-sample and pre-harvest; (3) patch-sample andpublic; and (4) patch-sample, pre-harvest, and public. Dependingon the foraging environment we presented to the starlings, eachassessment strategy made a unique set of predictions concerningthe patch departure decisions of pairs of birds based on differencesin their foraging success. The environment was manipulated intwo ways: by altering the variability in patch quality and bychanging compatibility, the ease with which individual birdscould simultaneously acquire both patch-sample and public information.Our observations on patch persistence and departure order demonstratethat the starlings used a combination of patch-sample and publicinformation, but not pre-harvest information, to estimate thequality of the experimental patch. Moreover, our results suggestthat starlings use public information only when it is easilyavailable and ignore it under incompatible conditions. Thisstudy provides the first evidence of public information usein a patch assessment problem. 相似文献
96.
A molecular phylogeny of New World emballonurid bats based on parsimony and Bayesian analyses of loci from the three different
nuclear genetic transmission pathways in mammals (autosomal, X, and Y chromosomes) is well supported and independently corroborated by each individual gene tree. This is in contrast to a single
most parsimonious but poorly supported tree based on morphological data, which has only one intergeneric or higher relationship
shared with the molecular phylogeny. Combining the morphological and molecular data partitions results in a tree similar to
the molecular tree suggesting a high degree of homoplasy and low phylogenetic signal in the morphological data set. Behavioral
data are largely incomplete and likewise produce a poorly resolved tree. Nonetheless, patterns of evolution in morphology
and behavior can be investigated by using the molecular tree as a phylogenetic framework. Character optimization of the appearance
of dorsal fur and preferred roosting sites maps consistently and are correlated on the phylogeny. This suggests an association
of camouflage for bats with unusual appearance (two dorsal stripes in Rhynchonycteris and Saccopteryx, or pale fur in Cyttarops and Diclidurus) and roosting in exposed sites (tree trunks or under palm leaves). In contrast, the ancestral states for Old and New World
emballonurids are typically uniform brown or black, and they usually roost in sheltered roosts such as caves and tree hollows.
Emballonuridae is the only family of bats that has a sac-like structure in the wing propatagium, which is found in four New
World genera. Mapping the wing sac character states onto the phylogeny indicates that wing sacs evolved independently within
each genus and that there may be a phylogenetic predisposition for this structure. Ear orientation maps relatively consistently
on the molecular phylogeny and is correlated to echolocation call parameters and foraging behavior, suggesting a phylogenetic
basis for these character systems. 相似文献
97.
In vitro fertilization has overcome infertility issues for many couples. However, achieving implantation of a viable embryo into the maternal endometrium remains a limiting step in optimizing pregnancy success. The molecular mechanisms which characterize the transient state of endometrial receptivity, critical in enabling embryo‐endometrial interactions, and proteins which underpin adhesion at the implantation interface, are limited in humans despite these temporally regulated processes fundamental to life. Hence, failure of implantation remains the “final frontier” in infertility. A human coculture model is utilized utilizing spheroids of a trophectoderm (trophoblast stem) cell line, derived from pre‐implantation human embryos, and primary human endometrial epithelial cells, to functionally identify “fertile” versus “infertile” endometrial epithelium based on adhesion between these cell types. Quantitative proteomics identified proteins associated with human endometrial epithelial receptivity (“epithelial receptome”) and trophectoderm adhesion (“adhesome”). As validation, key “epithelial receptome” proteins (MAGT‐1/CDA/LGMN/KYNU/PC4) localized to the epithelium of receptive phase (mid‐secretory) endometrium obtained from fertile, normally cycling women but is largely absent from non‐receptive (proliferative) phase tissues. Factors involved in embryo‐epithelium interaction in successive temporal stages of endometrial receptivity and implantation are demonstrated and potential targets for improving fertility are provided, enhancing potential to become pregnant either naturally or in a clinical setting. 相似文献
98.
Genea Edwards Jennifer Arcuri Haiyan Wang Noel Ziebarth Gulab Zode Richard K. Lee Sanjoy K. Bhattacharya 《Journal of cellular and molecular medicine》2020,24(7):3856-3900
Elevated intraocular pressure (IOP) is a risk factor in glaucoma, a group of irreversible blinding diseases. Endogenous lipids may be involved in regulation of IOP homeostasis. We present comparative fold analysis of phospholipids and sphingolipids of aqueous humour and trabecular meshwork from human control vs primary open-angle glaucoma and mouse control (normotensive) vs ocular hypertensive state. The fold analysis in control vs disease state was based on ratiometric mass spectrometric data for above classes of lipids. We standardized in vitro assays for rapid characterization of lipids undergoing significant diminishment in disease state. Evaluation of lipids using in vitro assays helped select a finite number of lipids that may potentially expand cellular interstitial space embedded in an artificial matrix or increase fluid flow across a layer of cells. These assays reduced a number of lipids for initial evaluation using a mouse model, DBA/2J with spontaneous IOP elevation. These lipids were then used in other mouse models for confirmation of IOP lowering potential of a few lipids that were found promising in previous assessments. Our results provide selected lipid molecules that can be pursued for further evaluation and studies that may provide insight into their function. 相似文献
99.
Amy E. Zanne Kessy Abarenkov Michelle E. Afkhami Carlos A. Aguilar‐Trigueros Scott Bates Jennifer M. Bhatnagar Posy E. Busby Natalie Christian William K. Cornwell Thomas W. Crowther Habacuc Flores‐Moreno Dimitrios Floudas Romina Gazis David Hibbett Peter Kennedy Daniel L. Lindner Daniel S. Maynard Amy M. Milo Rolf Henrik Nilsson Jeff Powell Mark Schildhauer Jonathan Schilling Kathleen K. Treseder 《Biological reviews of the Cambridge Philosophical Society》2020,95(2):409-433
Fungi play many essential roles in ecosystems. They facilitate plant access to nutrients and water, serve as decay agents that cycle carbon and nutrients through the soil, water and atmosphere, and are major regulators of macro‐organismal populations. Although technological advances are improving the detection and identification of fungi, there still exist key gaps in our ecological knowledge of this kingdom, especially related to function . Trait‐based approaches have been instrumental in strengthening our understanding of plant functional ecology and, as such, provide excellent models for deepening our understanding of fungal functional ecology in ways that complement insights gained from traditional and ‐omics‐based techniques. In this review, we synthesize current knowledge of fungal functional ecology, taxonomy and systematics and introduce a novel database of fungal functional traits (FunFun). FunFun is built to interface with other databases to explore and predict how fungal functional diversity varies by taxonomy, guild, and other evolutionary or ecological grouping variables. To highlight how a quantitative trait‐based approach can provide new insights, we describe multiple targeted examples and end by suggesting next steps in the rapidly growing field of fungal functional ecology. 相似文献
100.