Ultrastructural localization of Thermoplasma acidophilum surface carbohydrate by using concanavalin A

Surface carbohydrate, presumably the lipopolysaccharide, of Thermoplasma acidophilum was visualized by means of the concanavalin A, horseradish peroxidase, and diaminobenzidine cytochemical staining procedure.     

Methylcarbamate effects on Meckel's cartilages in the chick embryo

On the basis of previous observations on the teratogenic effects of a variety of organophosphorus and methylcarbamate compounds on the avian skeletal apparatus, the Meckel's cartilage shape and structure were analyzed in carbaryl (1-naphthyl N-methylcarbamate) treated and control chick embryos of 9, 10, 12 days of incubation. The results indicate that both during normal development and under experimental conditions these cartilages undergo similar deformities, apparently subsequent to chondroblast death and regressive processes in the extracellular matrix. Since the macro- and microscopical cartilage alterations are significantly more frequent in the treated embryos than in the controls, a hypothesis is advanced that the methylcarbamate may increase the spontaneous tendency of the above mentioned cartilaginous anlagen to be affected by degenerative processes during embryogenesis.     

Proton conductance of cell membranes

Several workers have suggested that cell membranes have a high proton conductance. Our interest in this concept arose from the possibility that the nutrient (submucosal-facing) membrane of the gastric mucosa may have a high proton or hydroxyl ion conductance which would play a role in the regulation of the acid-base balance of the cell. We found that wide changes in the H⁺ concentration of the fluid bathing the nutrient side of the in vitro frog gastric mucosa did not result in significant changes in p.d. However, a maintained change of the H⁺ concentration of the bathing fluid would be expected to produce only a temporary change in p.d. Since a diffusion barrier is present on the nutrient side the temporary change in p.d. might be masked. An analysis of this possibility was made on the basis of a conceptual model and as a result of the analysis it is concluded that the proton (and/or OH^?) conductance of the nutrient membrane of the frog gastric mucosa is not a significant fraction of its total conductance. The present status of the proton conductance hypothesis with respect to striated muscle and to the secretory membrane of the gastric mucosa is reviewed.