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741.
742.
Saleemulla Mahammad Jelena DinicJeremy Adler Ingela Parmryd 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2010,1801(6):625-634
Acute cholesterol depletion is generally associated with decreased or abolished T cell signalling but it can also cause T cell activation. This anomaly has been addressed in Jurkat T cells using progressive cholesterol depletion with methyl-beta-cyclodextrin (MBCD). At depletion levels higher than 50% there is substantial cell death, which explains reports of signalling inhibition. At 10–20% depletion levels, tyrosine phosphorylation is increased, ERK is activated and there is a small increase in cytoplasmic Ca2+. Peripheral actin polymerisation is also triggered by limited cholesterol depletion. Strikingly, the lipid raft marker GM1 aggregates upon cholesterol depletion and these aggregated domains concentrate the signalling proteins Lck and LAT, whereas the opposite is true for the non lipid raft marker the transferrin receptor. Using PP2, an inhibitor of Src family kinase activation, it is demonstrated that the lipid raft aggregation occurs independently of and thus upstream of the signalling response. Upon cholesterol depletion there is an increase in overall plasma membrane order, indicative of more ordered domains forming at the expense of disordered domains. That cholesterol depletion and not unspecific effects of MBCD was behind the reported results was confirmed by performing all experiments with MBCD–cholesterol, when no net cholesterol extraction took place. We conclude that non-lethal cholesterol depletion causes the aggregation of lipid rafts which then induces T cell signalling. 相似文献
743.
744.
Jelena Brkljačić Nikola Tanić Danijela Vojnović Milutinović Ivana Elaković Sanja Manitašević Jovanović Tatjana Perišić Jadranka Dundjerski Gordana Matić 《BMC molecular biology》2010,11(1):26
Background
Selection of appropriate endogenous control is a critical step in gene expression analysis. The aim of this study was to evaluate expression stability of four frequently used endogenous controls: β-actin, glyceraldehyde-3-phosphate dehydrogenase, β2-microglobulin and RNA polymerase II polypeptide A in peripheral blood mononuclear cells from war veterans with and without posttraumatic stress disorder (PTSD). The study was designed as to identify suitable reference gene(s) for normalization of gene expression in peripheral blood mononuclear cells in response to war trauma and/or PTSD. 相似文献745.
746.
Restriction enzymes have proven to be among the most valuable tools in molecular biology. In this work, we demonstrate that the cleavage of fluorescently labeled, PCR-amplified DNA can be used as a simple and highly sensitive technique for detection of sequences present in a percentage as low as 0.6% in a DNA pool. Due to the fact that fluorescent labeling of DNA fragments enables such sensitive detection and quantification of restriction enzyme cleavage, the method was further exploited in monitoring of the enzymatic digestion completeness and in determination of factors that influence restriction enzyme effectiveness. We analyzed the activity of six restriction endonucleases; the percentage of uncleaved DNA fragments predominantly ranged between 2.0 and 2.5 and the highest value was 8.00%. We conclude that, since the enzymatic digestion completeness may not always be assured, each assay based on restriction enzyme cleavage that is intended to be used in investigations of heterogeneity in a DNA pool should be constructed so that the presence of cleaved sequences is the indication of pool nonuniformity. When the presence of uncleaved sequences indicates pool heterogeneity, the results could be misleading due to possible incompleteness of enzymatic cleavage. 相似文献
747.
748.
Sereikaite J Jachno J Santockyte R Chmielevski P Bumelis VA Dienys G 《The protein journal》2006,25(6):369-378
About 14 proteins were tested for specific oxidative scission catalyzed by metal ions in the presence of ascorbate and oxidizing agents (O2 or hydrogen peroxide). Only four of them were degraded by Fe3+/Fe2+- ascorbate, twelve – by Cu2+/Cu+-ascorbate and two proteins (α- and β-caseins) were degraded by Pd2+ ions. The rate and the intensity of degradation are very different for various proteins. For the most of tested proteins only a small fraction of molecules was degraded. None of them was degraded completely. Two possible reasons of protein stability against oxidative degradation may be proposed as follows: either there is no metal binding site in a protein molecule, or metal binding ligands of protein undergo a rapid oxidative modification and the metal ion is released from the binding site. Human growth hormone was cut specifically at two sites by Cu2+/Cu+-ascorbate system. At least one of amino acid residues of this protein was modified by formation of reactive carbonyl. 相似文献
749.
Greta Burneikaitė Evgeny Shkolnik Jelena Čelutkienė Gitana Zuozienė Irena Butkuvienė Birutė Petrauskienė Pranas Šerpytis Aleksandras Laucevičius Amir Lerman 《Cardiovascular ultrasound》2017,15(1):11
Aim
To systematically review currently available cardiac shock-wave therapy (CSWT) studies in humans and perform meta-analysis regarding anti-anginal efficacy of CSWT.Methods
The Cochrane Controlled Trials Register, Medline, Medscape, Research Gate, Science Direct, and Web of Science databases were explored. In total 39 studies evaluating the efficacy of CSWT in patients with stable angina were identified including single arm, non- and randomized trials. Information on study design, subject’s characteristics, clinical data and endpoints were obtained. Assessment of publication risk of bias was performed and heterogeneity across the studies was calculated by using random effects model.Results
Totally, 1189 patients were included in 39 reviewed studies, with 1006 patients treated with CSWT. The largest patient sample of single arm study consisted of 111 patients. All selected studies demonstrated significant improvement in subjective measures of angina symptoms and/or quality of life, in the majority of studies left ventricular function and myocardial perfusion improved. In 12 controlled studies with 483 patients included (183 controls) angina class, Seattle Angina Questionnaire (SAQ) score, nitrates consumption were significantly improved after the treatment.In 593 participants across 22 studies the exercise capacity was significantly improved after CSWT, as compared with the baseline values (in meta-analysis standardized mean difference SMD?=??0.74; 95% CI, ?0.97 to ?0.5; p?<?0.001).Conclusions
Systematic review of CSWT studies in stable coronary artery disease (CAD) demonstrated consistent improvement of clinical variables. Meta-analysis showed a moderate improvement of exercise capacity.Overall, CSWT is a promising non-invasive option for patients with end-stage CAD, but evidence is limited to small sample single-center studies. Multi-center adequately powered randomised double blind studies are warranted.750.
Miloš Popović Martina Šašić Ivan Medenica Jelena Šeat Aca Đurđević Jelka Crnobrnja-Isailović 《Journal of Insect Conservation》2017,21(3):401-409
Due to their complex and interesting life cycle and alarming conservation status the Large Blue butterflies have become one of the most studied group of insects in Europe. In Serbia, however, Phengaris teleius has recently been discovered (in the far north of the country) and, since this initial finding, significant efforts have been made to map the local distribution of this species and to implement conservation measures. A mark-release-recapture study was initiated to obtain a more detailed report about population size and structure in Serbia. Results have shown that localities and patches within these localities are well connected by migration of butterflies and gave some evidence for metapopulation organisation. The total number of individuals was estimated at 15,000, which makes it one of the largest known metapopulations in Europe. Unusual for populations at the edge of the distribution range, these are characterized with large population estimates and relative stability. Compared to other areas in Europe, butterflies in Serbia start to fly 2 weeks later in the summer, probably a consequence of a late mowing regime initiated during July. Some evidence of a negative relationship between the survival of P. teleius adults and local population density was also found. With favourable management, these populations could remain stable for the foreseeable future. The preservation of large, healthy populations of P. teleius supported by a favourable management and monitoring programme along with the sustainability of individual dispersal translocation between patches should be a prime aim. 相似文献