全文获取类型
收费全文 | 16241篇 |
免费 | 1317篇 |
国内免费 | 2篇 |
出版年
2023年 | 48篇 |
2022年 | 146篇 |
2021年 | 272篇 |
2020年 | 124篇 |
2019年 | 219篇 |
2018年 | 185篇 |
2017年 | 201篇 |
2016年 | 377篇 |
2015年 | 623篇 |
2014年 | 740篇 |
2013年 | 891篇 |
2012年 | 1233篇 |
2011年 | 1295篇 |
2010年 | 833篇 |
2009年 | 693篇 |
2008年 | 1093篇 |
2007年 | 1139篇 |
2006年 | 959篇 |
2005年 | 959篇 |
2004年 | 932篇 |
2003年 | 946篇 |
2002年 | 846篇 |
2001年 | 142篇 |
2000年 | 103篇 |
1999年 | 147篇 |
1998年 | 203篇 |
1997年 | 148篇 |
1996年 | 123篇 |
1995年 | 119篇 |
1994年 | 116篇 |
1993年 | 124篇 |
1992年 | 119篇 |
1991年 | 104篇 |
1990年 | 90篇 |
1989年 | 77篇 |
1988年 | 76篇 |
1987年 | 68篇 |
1986年 | 66篇 |
1985年 | 91篇 |
1984年 | 102篇 |
1983年 | 84篇 |
1982年 | 84篇 |
1981年 | 101篇 |
1980年 | 90篇 |
1979年 | 54篇 |
1978年 | 55篇 |
1977年 | 61篇 |
1976年 | 49篇 |
1975年 | 32篇 |
1973年 | 27篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
851.
Schiegg K Pasinelli G Walters JR Daniels SJ 《Proceedings. Biological sciences / The Royal Society》2002,269(1496):1153-1159
In recent decades, female red-cockaded woodpeckers (Picoides borealis) have laid eggs increasingly earlier in response to a changing climate, as has been observed in several other bird species breeding at north temperate latitudes. Within each year, females that lay earlier are more productive than females that lay later. However, inexperienced females, experienced females who change mates and inbred birds have not adjusted to the changing climate by laying earlier, and have suffered reproductive costs as a result. Failure to respond to global climate change may be a further example of the reduced ability of inbred animals to respond to environmental challenges. 相似文献
852.
A plant pathogen reduces the enemy-free space of an insect herbivore on a shared host plant 总被引:6,自引:0,他引:6
Biere A Elzinga JA Honders SC Harvey JA 《Proceedings. Biological sciences / The Royal Society》2002,269(1506):2197-2204
An important mechanism in stabilizing tightly linked host-parasitoid and prey-predator interactions is the presence of refuges that protect organisms from their natural enemies. However, the presence and quality of refuges can be strongly affected by the environment. We show that infection of the host plant Silene latifolia by its specialist fungal plant pathogen Microbotryum violaceum dramatically alters the enemy-free space of a herbivore, the specialist noctuid seed predator Hadena bicruris, on their shared host plant. The pathogen arrests the development of seed capsules that serve as refuges for the herbivore's offspring against the specialist parasitoid Microplitis tristis, a major source of mortality of H. bicruris in the field. Pathogen infection resulted both in lower host-plant food quality, causing reduced adult emergence, and in twofold higher rates of parasitism of the herbivore. We interpret the strong oviposition preference of H. bicruris for uninfected plants in the field as an adaptive response, positioning offspring on refuge-rich, high-quality hosts. To our knowledge, this is the first demonstration that plant-inhabiting micro-organisms can affect higher trophic interactions through alteration of host refuge quality. We speculate that such interference can potentially destabilize tightly linked multitrophic interactions. 相似文献
853.
854.
The cardiac mechanical stretch sensor machinery involves a Z disc complex that is defective in a subset of human dilated cardiomyopathy 总被引:36,自引:0,他引:36
Knöll R Hoshijima M Hoffman HM Person V Lorenzen-Schmidt I Bang ML Hayashi T Shiga N Yasukawa H Schaper W McKenna W Yokoyama M Schork NJ Omens JH McCulloch AD Kimura A Gregorio CC Poller W Schaper J Schultheiss HP Chien KR 《Cell》2002,111(7):943-955
Muscle cells respond to mechanical stretch stimuli by triggering downstream signals for myocyte growth and survival. The molecular components of the muscle stretch sensor are unknown, and their role in muscle disease is unclear. Here, we present biophysical/biochemical studies in muscle LIM protein (MLP) deficient cardiac muscle that support a selective role for this Z disc protein in mechanical stretch sensing. MLP interacts with and colocalizes with telethonin (T-cap), a titin interacting protein. Further, a human MLP mutation (W4R) associated with dilated cardiomyopathy (DCM) results in a marked defect in T-cap interaction/localization. We propose that a Z disc MLP/T-cap complex is a key component of the in vivo cardiomyocyte stretch sensor machinery, and that defects in the complex can lead to human DCM and associated heart failure. 相似文献
855.
Prickle mediates feedback amplification to generate asymmetric planar cell polarity signaling 总被引:10,自引:0,他引:10
Myosin-1c (also known as myosin-Ibeta) has been proposed to mediate the slow component of adaptation by hair cells, the sensory cells of the inner ear. To test this hypothesis, we mutated tyrosine-61 of myosin-1c to glycine, conferring susceptibility to inhibition by N(6)-modified ADP analogs. We expressed the mutant myosin-1c in utricular hair cells of transgenic mice, delivered an ADP analog through a whole-cell recording pipette, and found that the analog rapidly blocked adaptation to positive and negative deflections in transgenic cells but not in wild-type cells. The speed and specificity of inhibition suggests that myosin-1c participates in adaptation in hair cells. 相似文献
856.
857.
858.
Bertolini M Mason JB Beam SW Carneiro GF Sween ML Kominek DJ Moyer AL Famula TR Sainz RD Anderson GB 《Theriogenology》2002,58(5):973-994
This study was designed to characterize conceptus development based on pre- and postnatal measurements of in vivo- and in vitro-derived bovine pregnancies. In vivo-produced embryos were obtained after superovulation, whereas in vitro-produced embryos were derived from established procedures for bovine IVM, IVF and IVC. Blastocysts were transferred to recipients to obtain pregnancies of single (in vivo/singleton or in vitro/singleton groups) or twin fetuses (in vitro/twins group). Ultrasonographic examinations were performed weekly, from Day 30 of gestation through term. Videotaped images were digitized, and still-frames were used for the measurement of conceptus traits. Calves and fetal membranes (FM) were examined and measured upon delivery. In vitro-produced fetuses were smaller than in vivo controls (P < 0.05) during early pregnancy (Day 37 to Day 58), but in vitro/singletons presented significantly higher weights at birth than in vivo/control and in vitro/twin calves (P < 0.05). From late first trimester of pregnancy (Day 72 to Day 93), placentomes surrounding in vitro-derived singleton fetuses were longer and thinner than controls (P < 0.05). At term, the presence of giant cotyledons in the fetal membranes in the in vitro group was associated with a larger cotyledonary surface area in the fetal horn (P < 0.05). The biphasic growth pattern seen in in vitro-produced pregnancies was characterized by conceptus growth retardation during early pregnancy, followed by changes in the development of the placental tissue. Resulting high birth weights may be a consequence of aberrant placental development due to the disruption of the placental restraint on fetal growth toward the end of pregnancy. 相似文献
859.
Alternanase, an endoglucanase that hydrolyzes the bacterial exopolysaccharide alternan, will also hydrolyze the trisaccharide, panose, to produce glucose and a disaccharide that can be formed into a novel, cyclic tetrasaccharide. The glucose can then be selectively and quantitatively measured by enzyme-based reaction which forms the basis of a coupled enzyme assay to quantitate alternanase activity. By this method a preparation of alternanase purified by affinity chromatography on immobilized isomaltose had a maximum reaction rate (V
max) of 0.75 mol glucose min–1 and a K
m of 34 mM for panose. Two competitive inhibitors of alternanase activity were also evaluated using this coupled enzyme assay: isomaltose had a K
i of 94 mM while the cyclic tetrasaccharide had a K
i of 66 mM. 相似文献
860.