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891.
K riaa , H., A rthaud , J.F. & F ournaud , J. 1985 Contamination and bacterial retention capacity of beef carcasses at the abattoir. Journal of Applied Bacteriology 59 , 23–28.
The contamination of beef carcasses was studied together with the capacity of meat surfaces to retain bacteria along the processing line in the slaughter hall.The results showed that the contamination varied along the processing line, but that this pattern was essentially dependent on the contamination at the dressing station. It decreased or remained unchanged during the first 12 min and then increased, even without additional contamination. The contamination varied according to carcasses and micro-organisms studied and was not greatly affected by spray cleaning. The number of bacteria retained changed at a rate similar to that of the contaminants. The attachment was instantaneous. The results are discussed and compared with the various hypotheses about contamination and bacterial attachment processes. 相似文献
The contamination of beef carcasses was studied together with the capacity of meat surfaces to retain bacteria along the processing line in the slaughter hall.The results showed that the contamination varied along the processing line, but that this pattern was essentially dependent on the contamination at the dressing station. It decreased or remained unchanged during the first 12 min and then increased, even without additional contamination. The contamination varied according to carcasses and micro-organisms studied and was not greatly affected by spray cleaning. The number of bacteria retained changed at a rate similar to that of the contaminants. The attachment was instantaneous. The results are discussed and compared with the various hypotheses about contamination and bacterial attachment processes. 相似文献
892.
893.
894.
Mark J. Rieder Glenn E. Green Sarah S. Park Brendan D. Stamper Christopher T. Gordon Jason M. Johnson Christopher M. Cunniff Joshua D. Smith Sarah B. Emery Stanislas Lyonnet Jeanne Amiel Muriel Holder Andrew A. Heggie Michael J. Bamshad Deborah A. Nickerson Timothy C. Cox Anne V. Hing Jeremy A. Horst Michael L. Cunningham 《American journal of human genetics》2012,90(6):1116-1117
895.
Cytogenetic, morphologic and oncogene analysis of a cell line derived from a heterologous mixed mullerian tumor of the ovary 总被引:5,自引:0,他引:5
Jeanne L. Becker Peter R. Papenhausen Raymond H. Widen 《In vitro cellular & developmental biology. Animal》1997,33(5):325-331
Summary A cell line was established from a mixed mullerian tumor of the ovary and designated LN1. Histopathologic analysis of the
fresh tumor specimen demonstrated a highly aneuploid heterologous tumor comprised of undifferentiated mesodermal components
with carcinomatous cells present as a smaller population. Long-term in vitro culture resulted in the establishment of a cell line that exhibits an epithelial-like morphology and expresses epithelial
antigens cytokeratin, epithelial membrane antigen, and carcinoma antigen TAG-72. These cells also express mesenchymal intermediate
filaments, vimentin, and desmin. Karyotypic analysis revealed a basic triploid pattern with multiple chromosomal abnormalities,
most notably an isochromosome of the short arm of five present in three copies. Analysis of oncogene expression revealed that
LN1 cells constitutively express mRNA for c-ras, c-erbB2, and p53. The expression of mRNA for cellular oncogenes correlated
with the presence of corresponding oncoproteins, p21H-ras, p21K-ras, and p185erbB2 and mutant p53 protein. In summary, coexpression of epithelial and mesenchymal antigens by LN1 cells lends support to the
hypothesis that epithelial and mesenchymal elements comprising mixed mullerian tumors of the ovary are derived from a common
stem cell precursor. Furthermore, this cell line represents a functional in vitro model to evaluate the biologic activities of these unusual and highly aggressive ovarian malignancies. 相似文献
896.
Michèle Dodeur Jean-Pierre Dumont Dominique Durand Geneviève Durand Jean Agneray Jeanne Feger 《Biochemical and biophysical research communications》1982,106(4):1169-1174
The initial velocity of asialoorosomucoid internalization is determined for normal and diabetic rat hepatocytes. The analysis of results according to Woolf-Hofstee's method, showed no modification of the endocytosis constant. In contrast, the maximum velocity of asialoorosomucoid internalization is decreased by threefold in diabetic rat hepatocytes as compared to normal rat hepatocytes. No modification of internalization constant is observed between the two groups of rats. This suggests that the decrease of asialoorosomucoid total uptake, previously reported for diabetic rat hepatocytes is directly related to a decrease of total surface receptor number. 相似文献
897.
Background
It has been proposed that high insulin levels may cause delayed lung development in the fetuses of diabetic mothers. A key event in lung development is the production of adequate amounts of pulmonary surfactant. Insulin inhibits the expression of surfactant protein A (SP-A), the major surfactant-associated protein, in lung epithelial cells. In the present study, we investigated the signal transduction pathways involved in insulin inhibition of SP-A gene expression. 相似文献898.
899.
Use of alternate substrates (acetylene, azide, cyanide) for estimating N(2) fixation confirmed that recombined fractions from different bacteria are functional but restricted in complementarity. 相似文献
900.