全文获取类型
收费全文 | 1176篇 |
免费 | 41篇 |
出版年
2022年 | 7篇 |
2021年 | 21篇 |
2020年 | 10篇 |
2019年 | 9篇 |
2018年 | 4篇 |
2017年 | 10篇 |
2016年 | 19篇 |
2015年 | 41篇 |
2014年 | 41篇 |
2013年 | 97篇 |
2012年 | 86篇 |
2011年 | 84篇 |
2010年 | 45篇 |
2009年 | 58篇 |
2008年 | 84篇 |
2007年 | 79篇 |
2006年 | 53篇 |
2005年 | 63篇 |
2004年 | 66篇 |
2003年 | 54篇 |
2002年 | 47篇 |
2001年 | 25篇 |
2000年 | 14篇 |
1999年 | 21篇 |
1998年 | 8篇 |
1997年 | 11篇 |
1996年 | 15篇 |
1995年 | 11篇 |
1994年 | 8篇 |
1993年 | 14篇 |
1992年 | 10篇 |
1991年 | 12篇 |
1990年 | 10篇 |
1989年 | 5篇 |
1988年 | 6篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 7篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1971年 | 3篇 |
1969年 | 2篇 |
1938年 | 3篇 |
1937年 | 4篇 |
1936年 | 5篇 |
排序方式: 共有1217条查询结果,搜索用时 31 毫秒
51.
Flore Samaran Kathleen M. Stafford Trevor A. Branch Jason Gedamke Jean-Yves Royer Robert P. Dziak Christophe Guinet 《PloS one》2013,8(8)
Understanding the seasonal movements and distribution patterns of migratory species over ocean basin scales is vital for appropriate conservation and management measures. However, assessing populations over remote regions is challenging, particularly if they are rare. Blue whales (Balaenoptera musculus spp) are an endangered species found in the Southern and Indian Oceans. Here two recognized subspecies of blue whales and, based on passive acoustic monitoring, four “acoustic populations” occur. Three of these are pygmy blue whale (B.m. brevicauda) populations while the fourth is the Antarctic blue whale (B.m. intermedia). Past whaling catches have dramatically reduced their numbers but recent acoustic recordings show that these oceans are still important habitat for blue whales. Presently little is known about the seasonal movements and degree of overlap of these four populations, particularly in the central Indian Ocean. We examined the geographic and seasonal occurrence of different blue whale acoustic populations using one year of passive acoustic recording from three sites located at different latitudes in the Indian Ocean. The vocalizations of the different blue whale subspecies and acoustic populations were recorded seasonally in different regions. For some call types and locations, there was spatial and temporal overlap, particularly between Antarctic and different pygmy blue whale acoustic populations. Except on the southernmost hydrophone, all three pygmy blue whale acoustic populations were found at different sites or during different seasons, which further suggests that these populations are generally geographically distinct. This unusual blue whale diversity in sub-Antarctic and sub-tropical waters indicates the importance of the area for blue whales in these former whaling grounds. 相似文献
52.
Jean-Yves Zimmer Fran?ois Smeets Grégory Simonon Jean Fagot Eric Haubruge Frédéric Francis Bertrand Losson 《PloS one》2013,8(6)
Several species of Culicoides (Diptera: Ceratopogonidae) biting midges serve as biological vectors for the bluetongue virus (BTV) and the recently described Schmallenberg virus (SBV) in northern Europe. Since their recent emergence in this part of the continent, these diseases have caused considerable economic losses to the sheep and cattle industries. Much data is now available that describe the distribution, population dynamics, and feeding habits of these insects. However, little is known regarding the presence of Culicoides in unusual habitats such as peaty marshes, nor their potential vector capacity. This study evaluated Culicoides biting midges present in the bogs of a Belgian nature reserve compared to those residing at a nearby cattle farm. Culicoides were trapped in 2011 at four different sites (broadleaved and coniferous forested areas, open environments, and at a scientific station) located in the Hautes Fagnes Nature Reserve (Belgium). An additional light trap was operated on a nearby cattle farm. Very high numbers of biting midges were captured in the marshy area and most of them (70 to 95%) were Culicoides impunctatus, a potential vector of BTV and other pathogens. In addition, fewer numbers of C. obsoletus/C. scoticus species, C. chiopterus, and C. dewulfi were observed in the bogs compared to the farm. The wet environment and oligotrophic nature of the soil were probably responsible for these changes in the respective populations. A total of 297,808 Culicoides midges belonging to 27 species were identified during this study and 3 of these species (C. sphagnumensis, C. clintoni and C. comosioculatus) were described in Belgium for the first time. 相似文献
53.
Mia Svensk Sabrina Coste Bastien Gérard Eva Gril Frédéric Julien Pascale Maillard Clément Stahl Céline Leroy 《Physiologia plantarum》2020,170(4):488-507
Studying the response to drought stress of keystone epiphytes such as tank bromeliads is essential to better understand their resistance capacity to future climate change. The objective was to test whether there is any variation in the carbon, water and nutrient status among different leaf ontogenetic stages in a bromeliad rosette subjected to a gradient of drought stress. We used a semi-controlled experiment consisting in a gradient of water shortage in Aechmea aquilega and Lutheria splendens. For each bromeliad and drought treatment, three leaves were collected based on their position in the rosette and several functional traits related to water and nutrient status, and carbon metabolism were measured. We found that water status traits (relative water content, leaf succulence, osmotic and midday water potentials) and carbon metabolism traits (carbon assimilation, maximum quantum yield of photosystem II, chlorophyll and starch contents) decreased with increasing drought stress, while leaf soluble sugars and carbon, nitrogen and phosphorus contents remained unchanged. The different leaf ontogenetic stages showed only marginal variations when subjected to a gradient of drought. Resources were not reallocated between different leaf ontogenetic stages but we found a reallocation of soluble sugars from leaf starch reserves to the root system. Both species were capable of metabolic and physiological adjustments in response to drought. Overall, this study advances our understanding of the resistance of bromeliads faced with increasing drought stress and paves the way for in-depth reflection on their strategies to cope with water shortage. 相似文献
54.
55.
56.
57.
58.
Bianca Audrain Lionel Ferrières Amira Zairi Guillaume Soubigou Curtis Dobson Jean-Yves Coppée Christophe Beloin Jean-Marc Ghigo 《Applied and environmental microbiology》2013,79(24):7770-7779
Antimicrobial peptides produced by multicellular organisms as part of their innate system of defense against microorganisms are currently considered potential alternatives to conventional antibiotics in case of infection by multiresistant bacteria. However, while the mode of action of antimicrobial peptides is relatively well described, resistance mechanisms potentially induced or selected by these peptides are still poorly understood. In this work, we studied the mechanisms of action and resistance potentially induced by ApoEdpL-W, a new antimicrobial peptide derived from human apolipoprotein E. Investigation of the genetic response of Escherichia coli upon exposure to sublethal concentrations of ApoEdpL-W revealed that this antimicrobial peptide triggers activation of RcsCDB, CpxAR, and σE envelope stress pathways. This genetic response is not restricted to ApoEdpL-W, since several other antimicrobial peptides, including polymyxin B, melittin, LL-37, and modified S4 dermaseptin, also activate several E. coli envelope stress pathways. Finally, we demonstrate that induction of the CpxAR two-component system directly contributes to E. coli tolerance toward ApoEdpL-W, polymyxin B, and melittin. These results therefore show that E. coli senses and responds to different antimicrobial peptides by activation of the CpxAR pathway. While this study further extends the understanding of the array of peptide-induced stress signaling systems, it also provides insight into the contribution of Cpx envelope stress pathway to E. coli tolerance to antimicrobial peptides. 相似文献
59.
60.
Thomas Arnauld Jean-Yves Beaumal François Lefoulon Alain Petit Tristan Renaud 《Bioorganic & medicinal chemistry letters》2013,23(7):2217-2219
During the course of our study on the innovative ligand for nicotinic acetylcholinergic receptors, LNAChR, and in order to assess activity and toxicity profiles of the drug’s metabolites, synthesis of the main metabolites was undertaken. This synthesis work was done in parallel by organic chemistry and by biotransformation of LNAChR. Filamentous fungus Aspergillus alliaceus (NRRL 315) neatly afforded three of the main metabolites, one of which arose from a very unexpected and very uncommon rearrangement. 相似文献