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801.
Marion Picard Satoru Yonekura Karolina Slowicka Ioanna Petta Conrad Rauber Bertrand Routy Corentin Richard Valerio Iebba Maryam Tidjani Alou Sonia Becharef Pierre Ly Eugenie Pizzato Christian H. K. Lehmann Lukas Amon Christophe Klein Paule Opolon Ivo Gomperts Boneca Jean-Yves Scoazec Antoine Hollebecque David Malka Franois Ghiringhelli Diana Dudziak Geert Berx Lars Vereecke Geert van Loo Guido Kroemer Laurence Zitvogel Maria Paula Roberti 《Cell death and differentiation》2021,28(5):1532
802.
Jean-Yves Le Guennec Nicolas Peineau Frdric Esnard Alain Lacampagne Francois Gannier Jorge Argibay Francis Gauthier Didier Garnier 《Biology of the cell / under the auspices of the European Cell Biology Organization》1993,79(2):161-165
Summary— A mixture of crude collagenase and non-specific proteases has been used to isolate guinea pig ventricular heart cells. Measurements of collagenase activity with Wünsch's substrate and protein content with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) suggest that collagenase enzymes do not play a major role in heart cell isolation. On the other hand, an important factor in heart digestion seems to consist of some fractions of the proteases present in crude collagenase. It is also noted that crude collagenases do not present any sensitivity to added calcium but because this ion is important to obtain isolated cells its role is discussed. According to our results, the SDS-PAGE method can be used to determine the appropriate enzyme concentrations to obtain calcium-tolerant myocytes. These myocytes have electrophysiological properties as reported in the literature. 相似文献
803.
Christiane Durieux Joël Belleney Jean-Yves Lallemand Bernard P. Roques Marie-Claude Fournie-Zaluski 《Biochemical and biophysical research communications》1983,114(2):705-712
1H NMR study of cholecystokinin fragment (CCK27–33) in (C2H3)2SO and in 2H2O at different pH shows that sulfated (CCK7) and non sulfated (NS-CCK7) peptides are under preferentially folded conformations characterized by a β-turn including the sequence Gly-Trp-Met-Asp with a H-bond between the CO of Gly and the NH of Asp. This structure is probably stabilized by an ionic interaction between Tyr and Asp. Moreover, the N-terminal part of CCK7 forms a C7 structure with a weak H-bond between the CO of Gly and the NH of Trp. In this model all CCK7 hydrophobic side chains are in close vicinity, far from the hydrophilic sulfate group. Full interaction with brain CCK8 receptors could require both the sulfate group and the maintening of conformational constraints. 相似文献
804.
Jean-Yves Sire 《Journal of morphology》1993,215(3):225-244
The development and the structure of the bony scutes have been studied in a growth series of the armored catfish Corydoras arcuatus using light and electron microscopy. Fibroblast-like cell condensations appear in the dermis, in the posterior region of the caudal peduncle, and these will constitute the scute papillae. Collagen bundles of the preexisting dermis colonized by the papilla cells are remodeled and incorporated in the papilla to form, in addition to newly synthesized woven-fibered bony material, the initium of the scute. This process of formation differs from that described for the dermal papilla of an elasmoid scale. During growth, the osteoblasts surrounding the scute constitute the scute sac in which the scute grows. Parallel-fibered bone is deposited on both sides of the initium, and osteoblasts are incorporated within the scute matrix. The remodeling and incorporation of collagen bundles of the preexisting dermis is maintained during growth only in the deep, anterior region of the scute. The posterior region and the upper surface of the scute are close to the epidermal-dermal boundary. When growth slows down in the upper part of the scute, a characteristic, well-mineralized tissue, composed of thin vertical fibrils and granules and devoid of typical striated collagen fibrils, is deposited on the scute surface. A new term, hyaloine, is introduced for this nonosseous, highly mineralized layer constituting the upper part of the scute. Hyaloine shows thin electron-dense lines, which probably correspond to periodic growth arrests. The structure and localization of the hyaloine are compared to other well-mineralized, similar tissues found on the surface of the dermal skeleton in lower vertebrates. © 1993 Wiley-Liss, Inc. 相似文献
805.
Chantal Barthomeuf Henri Pourrat Jean-Yves Berthon Dominique Agius 《Biotechnology Techniques》1994,8(12):895-900
Summary A protease fromAspergillus niger LCF 8 was purified in anion exchange mode using BioCAD Workstations and the process scaled up. The use of POROS 50 HQ media brings
the benefits of Perfusion chromatography i.e. high resolution and high dynamic capacity with very short cycle times. Results
obtained at pilot scale enable to purify at process scale 151g of protease/m3 crude broth in 1h. The small column size needed (2.25 l media/m3 crude broth) and the high processing speed of POROS* 50 HQ compared with conventional media lead to greatly improved cost effectiveness and higher product recovery. 相似文献