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991.
Pattamawan Chimma Christian Roussilhon Panudda Sratongno Ronnatrai Ruangveerayuth Kovit Pattanapanyasat Jean-Louis Pérignon David J. Roberts Pierre Druilhe 《PLoS pathogens》2009,5(10)
Monocyte (MO) subpopulations display distinct phenotypes and functions which can drastically change during inflammatory states. We hypothesized that discrete MO subpopulations are induced during malaria infection and associated with anti-parasitic activity. We characterized the phenotype of blood MO from healthy malaria-exposed individuals and that of patients with acute uncomplicated malaria by flow cytometry. In addition, MO defense function was evaluated by an in vitro antibody dependent cellular inhibition (ADCI) assay. At the time of admission, the percentages and absolute numbers of CD16+ MO, and CCR2+CX3CR1+ MO, were high in a majority of patients. Remarkably, expression of CCR2 and CX3CR1 on the CD14high (hi) MO subset defined two subgroups of patients that also differed significantly in their functional ability to limit the parasite growth, through the ADCI mechanism. In the group of patients with the highest percentages and absolute numbers of CD14hiCCR2+CX3CR1+ MO and the highest mean levels of ADCI activity, blood parasitemias were lower (0.14±0.34%) than in the second group (1.30±3.34%; p = 0.0053). Data showed that, during a malaria attack, some patients'' MO can exert a strong ADCI activity. These results bring new insight into the complex relationships between the phenotype and the functional activity of blood MO from patients and healthy malaria-exposed individuals and suggest discrete MO subpopulations are induced during malaria infection and are associated with anti-parasitic activity. 相似文献
992.
Jian-sheng Sun Jean-Louis Mergny Richard Lavery Thérèse Montenay-Garestier Claude Hélène 《Journal of biomolecular structure & dynamics》2013,31(3):411-424
Abstract By means of molecular modelling, electrostatic interactions are shown to play an important role in the sequence-dependent structure of triple helices formed by a homopyrimidine oligonucleotide bound to a homopurine, homopyrimidine sequence on DNA. This is caused by the presence of positive charges due to the protonation of cytosines in the Hoogsteen-bonded strand, required in order to form C.GxC+ triplets. Energetic and conformational characteristics of triple helices with different sequences are analyzed and discussed. The effects of duplex mismatches on the triple helix stability are investigated via thermal dissociation using UV absorption. 相似文献
993.
994.
Delphine Lamoral-Theys Laurent Pottier Frédéric Kerff François Dufrasne Fabien Proutière Nathalie Wauthoz Philippe Neven Laurent Ingrassia Pierre Van Antwerpen Florence Lefranc Michel Gelbcke Bernard Pirotte Jean-Louis Kraus Jean Nève Alexander Kornienko Robert Kiss Jacques Dubois 《Bioorganic & medicinal chemistry》2010,18(11):3823-3833
A series of 33 novel divanillates and trivanillates were synthesized and found to possess promising cytostatic rather than cytotoxic properties. Several compounds under study decreased by >50% the activity of Aurora A, B, and C, and WEE1 kinase activity at concentrations <10% of their IC50 growth inhibitory ones, accounting, at least partly, for their cytostatic effects in cancer cells and to a lesser extent in normal cells. Compounds 6b and 13c represent interesting starting points for the development of cytostatic agents to combat cancers, which are naturally resistant to pro-apoptotic stimuli, including metastatic malignancies. 相似文献
995.
A short historical survey recalls the main techniques of medical imaging, based on modern physico-chemistry and computer science. Imagery has provided novel visions of the inside of the body, which are not self-obvious but require a training of the gaze. Yet, these new images have permeated the contemporary mind and inspired esthetic ventures. The popularity of these images may be related to their ambiguous status, between real and virtual. The images, reminiscent of Vesalius' De humani corporis fabrica, crosslink art, science and society in a specific way: which role will they play in the "empowerment" of the tomorrow patient? 相似文献
996.
Azandégbé A Garnier M Andrieux-Loyer F Kérouel R Philippon X Nicolas JL 《Diseases of aquatic organisms》2010,91(3):213-221
Vibrio aestuarianus is frequently found in coastal areas and can infect and induce mortalities in the pacific oyster Crassostrea gigas. However, nothing is known about its distribution and seasonality in the estuarine environment, especially where oyster farming is practiced. Its occurrence was investigated in sediment and oyster haemolymph at 2 oyster farms in Brittany (France) over 2 yr during 2 periods, from June to September 2007 and from February to June 2008. Total heterotrophic bacteria (HB) were cultured on marine agar while total Vibrio spp. and V aestuarianus were selectively numerated using thiosulfate citrate bile salts sucrose agar (TCBS agar) and the species-specific hybridisation method, respectively. PCR was performed to detect V aestuarianus in sediment when it became unculturable. Both total Vibrio spp. and V aestuarianus had a seasonal trend. The highest concentrations were recovered in the warmest months. Its abundance ranged from 10(2) to 4 x 10(5) CFU ml(-1) in haemolymph and from 10(3) to 1 x 10(4) CFU g(-1) in the sediment. Temperature was the main factor influencing the concentration of Vibrio spp. and V. aestuarianus in the sediment. Thus V aestuarianus might subsist during the cold seasons in the sediment, from which it can emerge when environmental conditions became favourable. 相似文献
997.
We report on the neuroprotective effects of N,N-bis-(8-hydroxyquinoline-5-yl methyl)-benzyl substituted amines (HQNBA) in a model of oxidative stress-induced nerve cell death using mouse hippocampal-derived HT22 cells. The four derivatives (JLK1472, JLK1486, JLK1522 and JLK1535) protected the HT22 cells from death at concentrations ranging from 0.1 to 1 μM. Their action is partially dependent on their ability to act as PPARγ agonists. These analogues also maintain GSH levels suggesting that they have indirect anti-oxidant effects. 相似文献
998.
Ana Elena de Villalobos Diego P. Vázquez Jean-Louis Martin 《Biological invasions》2010,12(5):1023-1029
We evaluate the mechanisms that determine the establishment of the non-indigenous shrub Pyracantha coccinea (Rosaceae) in the Montpellier region of southern France. P. coccinea establishes in abandoned agricultural fields in this region; yet, despite its high propagule pressure, it has not become
a widespread invasive. We hypothesized that the disturbance conditions prevailing in abandoned agricultural fields right after
abandonment may enhance the emergence, survival and growth of P. coccinea, but that shortly after abandonment colonizing vegetation prevents further establishment of this species. We conducted a
field experiment to evaluate this hypothesis, studying the response of seedling emergence and growth of P. coccinea to soil and vegetation disturbance. Our results show that both lack of vegetation cover and soil disturbance promote the
emergence of seedlings of P. coccinea. Thus, the disturbance conditions prevailing in abandoned agricultural fields seem crucial to allow establishment of this
species. However, other factors such as lack of summer dormancy and seed predation might explain why this species has not
become a widespread invasive. 相似文献
999.
Benjamin Rey Damien Roussel Caroline Romestaing Maud Belouze Jean-Louis Rouanet Dominique Desplanches Brigitte Sibille Stéphane Servais Claude Duchamp 《BMC physiology》2010,10(1):5
Background
Although identified in several bird species, the biological role of the avian homolog of mammalian uncoupling proteins (avUCP) remains extensively debated. In the present study, the functional properties of isolated mitochondria were examined in physiological or pharmacological situations that induce large changes in avUCP expression in duckling skeletal muscle. 相似文献1000.