全文获取类型
收费全文 | 1009篇 |
免费 | 60篇 |
出版年
2022年 | 4篇 |
2021年 | 10篇 |
2019年 | 8篇 |
2018年 | 7篇 |
2017年 | 8篇 |
2016年 | 21篇 |
2015年 | 19篇 |
2014年 | 27篇 |
2013年 | 72篇 |
2012年 | 75篇 |
2011年 | 52篇 |
2010年 | 37篇 |
2009年 | 42篇 |
2008年 | 55篇 |
2007年 | 58篇 |
2006年 | 60篇 |
2005年 | 61篇 |
2004年 | 44篇 |
2003年 | 49篇 |
2002年 | 61篇 |
2001年 | 14篇 |
2000年 | 9篇 |
1999年 | 12篇 |
1998年 | 10篇 |
1997年 | 13篇 |
1996年 | 18篇 |
1995年 | 14篇 |
1994年 | 12篇 |
1993年 | 7篇 |
1992年 | 22篇 |
1991年 | 15篇 |
1990年 | 11篇 |
1989年 | 12篇 |
1988年 | 13篇 |
1987年 | 7篇 |
1986年 | 12篇 |
1985年 | 13篇 |
1984年 | 10篇 |
1983年 | 5篇 |
1982年 | 8篇 |
1981年 | 10篇 |
1980年 | 4篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1977年 | 9篇 |
1976年 | 7篇 |
1975年 | 4篇 |
1974年 | 10篇 |
1973年 | 2篇 |
1972年 | 3篇 |
排序方式: 共有1069条查询结果,搜索用时 15 毫秒
91.
Comparative maps of motion and assembly of filamentous actin and myosin II in migrating cells 总被引:1,自引:1,他引:0 下载免费PDF全文
Schaub S Bohnet S Laurent VM Meister JJ Verkhovsky AB 《Molecular biology of the cell》2007,18(10):3723-3732
To understand the mechanism of cell migration, one needs to know how the parts of the motile machinery of the cell are assembled and how they move with respect to each other. Actin and myosin II are thought to be the major structural and force-generating components of this machinery (Mitchison and Cramer, 1996; Parent, 2004). The movement of myosin II along actin filaments is thought to generate contractile force contributing to cell translocation, but the relative motion of the two proteins has not been investigated. We use fluorescence speckle and conventional fluorescence microscopy, image analysis, and computer tracking techniques to generate comparative velocity and assembly maps of actin and myosin II over the entire cell in a simple model system of persistently migrating fish epidermal keratocytes. The results demonstrate contrasting polarized assembly patterns of the two components, indicate force generation at the lamellipodium-cell body transition zone, and suggest a mechanism of anisotropic network contraction via sliding of myosin II assemblies along divergent actin filaments. 相似文献
92.
Charniot JC Bonnefont-Rousselot D Albertini JP Zerhouni K Dever S Richard I Nataf P Pavie A Monsuez JJ Delattre J Artigou JY 《Free radical research》2007,41(8):911-918
Xenotransplantation (XT) reveals a growing interest for the treatment of cardiomyopathy. The major barrier is an acute vascular rejection due to an acute humoral rejection. This pathogenesis is a difficult issue and in order to elaborate means for its prevention, we analysed the implication of oxidative stress (OS) on hearts from mini-pigs followed by reperfusion with either autologous or human blood in an attempt to simulate xenotransplantation. About 14 hearts were studied after a Langendorff blood reperfusion: allografts with autologous blood (n = 7) or xenografts with human blood (n = 7). Blood samples were drawn from the coronary sinus to assess ischemia and OS. In xenografts, arrhythmias occurred more frequently (p < 0.01, left ventricular systolic pressure decreased more significantly (p < 0.05), thiobarbituric acid-reactive substances concentrations increased at 30 min (0.7 +/- 0.1 vs. 2.4 +/- 0.3 mmol/l; p < 0.05) while vitamin A levels decreased (p < 0.05). XT was associated with a significant increase in ischemic injury and OS production. OS might play an eminent role in hyperacute humoral rejection. 相似文献
93.
94.
95.
Emilie Blanc Patrick Wagner Fabrice Plaisier Martine Schmitt Thierry Durroux Jean-Jacques Bourguignon Michel Partiseti Elodie Dupuis Frederic Bihel 《Analytical biochemistry》2015
Ligand-gated ion channels (LGICs) are considered as attractive protein targets in the search for new therapeutic agents. Nowadays, this strategy involves the capability to screen large chemical libraries. We present a new Tag-lite ligand binding assay targeting LGICs on living cells. This technology combines the use of suicide enzyme tags fused to channels of interest with homogeneous time-resolved fluorescence (HTRF) as the detection readout. Using the 5-HT3 receptor as system model, we showed that the pharmacology of the HALO-5HT3 receptor was identical to that of the native receptor. After validation of the assay by using 5-HT3 agonists and antagonists of reference, a pilot screen enabled us to identify azelastine, a well-known histamine H1 antagonist, as a potent 5-HT3 antagonist. This interesting result was confirmed with electrophysiological experiments. The method described here is easy to implement and could be applicable for other LGICs, opening new ways for the screening of chemical libraries. 相似文献
96.
Sergei I. Golovatch Jean-Jacques Geoffroy Jean-Paul Mauriès Didier VandenSpiegel 《ZooKeys》2015,(505):1-34
The Eutrichodesmus fauna of mainland China, by far the largest genus in the Indo-Australian family Haplodesmidae, is reviewed and shown to encompass 23 species (of a total of 45), all keyed. The following nine new species, all presumed troglobites, are described: Eutrichodesmus
triangularis
sp. n., from Sichuan, Eutrichodesmus
lipsae
sp. n., from Guangxi, Eutrichodesmus
tenuis
sp. n., Eutrichodesmus
trontelji
sp. n., Eutrichodesmus
latellai
sp. n., Eutrichodesmus
obliteratus
sp. n. and Eutrichodesmus
troglobius
sp. n., all from Guizhou, Eutrichodesmus
sketi
sp. n., from Hunan, and Eutrichodesmus
apicalis
sp. n., from Hubei. 相似文献
97.
98.
The number of stadia during post-embryonic development is supposed to be fixed in most species of the millipede order Polydesmida. For the first time since 1928, additional moults were observed in two males of Polydesmus angustus Latzel, 1884 reared in the laboratory. These 'elongatus' males sensu Verhoeff reached stadium IX instead of stadium VIII, with addition of a further podous ring (32 pairs of legs). One male had well-developed gonopods at stadium VIII, which regressed at stadium IX; the other had no gonopods at stadium VIII, which developed at stadium IX. The two cases correspond to the 'regressionis' and 'progressionis' forms described by Verhoeff in Polydesmus complanatus (Linnaeus, 1761), which confirms entirely his results. Additional moults appear to be associated with small body sizes and possible underlying mechanisms are discussed. Comparisons between millipede orders indicate that post-embryonic development is less strictly canalized in Polydesmida than in Chordeumatida. This implies that the adult number of body rings is of limited taxonomic value in Polydesmida and should not be viewed as a character of generic importance. 相似文献
99.
Bonhomme J Le Goff L Lemée V Gargala G Ballet JJ Favennec L 《Parasitology international》2011,60(3):327-330
The intestinal protozoan Giardia duodenalis includes 2 genetically distinct assemblages, A and B, which are responsible for human infections. Little is known so far on the genotypes of G. duodenalis human isolates in France. The present characterization of 19 French clinical isolates was aimed at determining their genotype patterns and associations with clinical symptoms, and in vivo metronidazole resistance, respectively. Based on both triose-phosphate isomerase (tpi) and β-giardin (bg) gene sequences, twelve isolates were identified as assemblage A, and 7 as assemblage B for the 2 gene loci. Sub-genotyping heterogeneities were observed in 15/19 isolates attributed to either A or B assemblage. They include frequent mismatches and intra-assemblage discordances and mixed positions, which were found more frequently in tpi than in bg sequences, and in assemblage B than in assemblage A sequences. No association was found between sub-genotypes, clinical symptoms and metronidazole sensitivity. Present data underline the need for improvements in the standardization of G. duodenalis multilocus genotyping approach for further molecular epidemiologic studies of giardiasis. 相似文献
100.
Auerbach AD Burn J Cassiman JJ Claustres M Cotton RG Cutting G den Dunnen JT El-Ruby M Vargas AF Greenblatt MS Macrae F Matsubara Y Rimoin DL Vihinen M Van Broeckhoven C 《Nature reviews. Genetics》2011,12(12):881; discussion 881