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11.
Post-translational modification of the beta-subunit of the human fibronectin receptor 总被引:1,自引:0,他引:1
M Jaspers B de Strooper M Spaepen F van Leuven G David H van den Berghe J J Cassiman 《FEBS letters》1988,231(2):402-406
Monoclonal antibody DH12, directed against the beta-subunit of the fibronectin receptor recognizes a doublet of proteins (100 and 110 kDa) in Western blots of solubilized whole fibroblasts. Pulse-chase experiments with [35S]methionine in human skin fibroblasts suggested that the two proteins might be metabolically related as precursor (100 kDa) and product (110 kDa). Endo H digestion and [3H]fucose labeling suggested that maturation converted the high-mannose oligosaccharides (100 kDa) to the endoglycosidase H resistant complex type (110 kDa). This was supported by N-glycanase digestion and by chemical deglycosylation which showed a single polypeptide. Surface iodination of intact cells labeled only the presumed mature beta-subunit. 相似文献
12.
Eveline De Mdicis Jean Paquette Jean-Jacques Gauthier Dennis Shapcott 《Applied microbiology》1986,52(3):567-573
Magnesium and manganese contents were measured by atomic absorption spectrophotometry in bacteria of several halophilic levels, in Vibrio costicola, a moderately halophilic eubacterium growing in 1 M NaCl, Halobacterium volcanii, a halophilic archaebacterium growing in 2.5 M NaCl, Halobacterium cutirubrum, an extremely halophilic archaebacterium growing in 4 M NaCl, and Escherichia coli, a nonhalophilic eubacterium growing in 0.17 M NaCl. Magnesium and manganese contents varied with the growth phase, being maximal at the early log phase. Magnesium and manganese molalities in cell water were shown to increase with the halophilic character of the logarithmically growing bacteria, from 30 mmol of Mg per kg of cell water and 0.37 mmol of Mn per kg of cell water for E. coli to 102 mmol of Mg per kg of cell water and 1.6 mmol of Mn per kg of cell water for H. cutirubrum. The intracellular concentrations of manganese were determined independently by a radioactive tracer technique in V. costicola and H. volcanii. The values obtained by 54Mn loading represented about 70% of the values obtained by atomic absorption. The increase of magnesium and manganese contents associated with the halophilic character of the bacteria suggests that manganese and magnesium play a role in haloadaptation. 相似文献
13.
Eduardo Agosin Jean-Jacques Daudin Etienne Odier 《Applied microbiology and biotechnology》1985,22(2):132-138
Summary 74 Basidiomycetes have been tested for ligninolytic capability on (14C)lignin-labelled wheat straw. Fifteen strains were selected and rested more accurately for ligninolytic activity and the capacity to degrade wheat straw. The asymptote, inflexion point and degradation rate were determined using a model approach. The fungi exhibited very different responses with respect to lignin biodegradation: high asymptote for Pleurotus ostreatus (77%), low inflexion points for Sporotrichum pulverulentum Nov. (6.1 days) and Pycnoporus spp. (2.7 to 4.7 days) with high and slow degradation rates, respectively (0.91% and 0.45% of 14CO2 release/day). Degradation values for (14C)whole-labelled wheat straw exhibited less variation. Finally, the strains Pleurotus ostreatus, Dichomitus squalens and Bjerkandera adusta showed the highest selectivity of lignin removal. 相似文献
14.
Jean-Jacques Fleury 《Geobios》1974,7(4):307-322
The formerly unknown microspheric form of Raadshoovenia salentina (Papetti and Tedeschi) and the new genus Cyclopseudedomia (type-species Cyclopseudedomia smouti n.g., n.sp.) are described from limestones of Campanian-Maastrichtian age from Gavrovo-Tripolitza zone in Greece. The new genus is distinguished from others of the sub-family RhapydionininaeKeijzer emend. Hamaoui and Fourcade by its almost entirely evolute test made of chambers tending to a cyclical stage (rapidly reached in the microspheric form). 相似文献
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We have studied 11 cases of acute human trichinosis; five of these patients were treated with thiabendazole. We have reviewed the laboratory data for these patients and described their course. This study has failed to show any definite effect of thiabendazole on the course of human trichinosis at the doses used. However, thiabendazole may have decreased the infectivity of the larvae which were embedded in the muscles of our patients. 相似文献
18.
Peter Raeymaekers Christine Van Broeckhoven Hubert Backhovens Anita Wehnert Ludo Muylle Peter De Jonghe Jan Ghenens Jean-Jacques Martin Antoon Vandenberghe 《Human genetics》1989,81(3):231-233
Summary We have recently demonstrated tight linkage of the Duffy blood group marker to the -spectrin gene in an extended pedigree with Charcot-Marie-Tooth neuropathy. To determine a more precise location of the Duffy blood group locus on the chromosome 1 map we have tested several more chromosome 1 genes for linkage with this marker. We found suggestive linkage with the antithrombin III and apolipoprotein A2 genes and conclusive linkage with the gene coding for -nerve growth factor. 相似文献
19.
Frenette Jean-Jacques; Demers Serge; Legendre Louis; Boule Michel 《Journal of plankton research》1996,18(1):45-61
Phytoplankton photosynthetic responses were studied in two basinsof an oligotrophic lake (Québec, Canada). which are characterizedby the absence (shallow Basin 1) and presence (deeper Basin2) of seasonal thermal stratification. Size-fractionated photosynthesiswas used to characterize changes in phytoplankton characteristicsduring periods of seasonal mixing and stratification. Seasonalvariations of P max showed size-related differences, with maximumvalues in July for the picoplankton and in November for thenanoplankton. Similar patterns of variability in 相似文献
20.
A new gene coding for an antigen recognized by autologous cytolytic T lymphocytes on a human renal carcinoma 总被引:7,自引:0,他引:7
Béatrice Gaugler Nathalie Brouwenstijn Valérie Vantomme Jean-Pierre Szikora Corry W. Van der Spek Jean-Jacques Patard Thierry Boon Peter Schrier Benoît J. Van den Eynde 《Immunogenetics》1996,44(5):323-330
Previous reports have described antigens that are recognized on human melanoma cells by autologous cytolytic T lymphocytes
(CTL). The genes coding for a number of these antigens have been identified. Here we report the cloning of a gene that codes
for an antigen recognized by autologous CTL on a human renal carcinoma cell line. This antigen is presented byHLA-B7 and is encoded by a new gene that we have namedRAGE1. No expression ofRAGE1 was found in normal tissues other than retina. RAGE1 expression was found in only one of 57 renal cell carcinoma samples,
and also in some sarcomas, infiltrating bladder carcinomas, and melanomas. This represents the first identification of an
antigen recognized by autologous CTL on a renal tumor. 相似文献