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271.
272.
Jean-Guy Berrin W Russell McLauchlan Paul Needs Gary Williamson Antoine Puigserver Paul A Kroon Nathalie Juge 《European journal of biochemistry》2002,269(1):249-258
Human tissues such as liver, small intestine, spleen and kidney contain a cytosolic beta-glucosidase (CBG) that hydrolyses various beta-d-glycosides, but whose physiological function is not known. Here, we describe the first heterologous expression of human CBG, a system that facilitated a detailed assessment of the enzyme specificity towards dietary glycosides. A full-length CBG cDNA (cbg-1) was cloned from a human liver cDNA library and expressed in the methylotrophic yeast Pichia pastoris at a secretion yield of approximately 10 mg x L-1. The recombinant CBG (reCBG) was purified from the supernatant using a single chromatography step and was shown to be similar to the native enzyme isolated from human liver in terms of physical properties and specific activity towards 4-nitrophenyl-beta-D-glucoside. Furthermore, the reCBG displayed a broad specificity with respect to the glycone moiety of various aryl-glycosides (beta-D-fucosides, alpha-L-arabinosides, beta-D-glucosides, beta-D-galactosides, beta-L-xylosides, beta-D-arabinosides), similar to the native enzyme. For the first time, we show that the human enzyme has significant activity towards many common dietary xenobiotics including glycosides of phytoestrogens, flavonoids, simple phenolics and cyanogens with higher apparent affinities (K(m)) and specificities (k(cat)/K(m)) for dietary xenobiotics than for other aryl-glycosides. These data indicate that human CBG hydrolyses a broad range of dietary glucosides and may play a critical role in xenobiotic metabolism. 相似文献
273.
The phenotypic data published by J. Frateur on 35 strains of acetic acid bacteria were analyzed numerically. The four clusters obtained correspond very well to Frateur's suboxydans, mesoxydans, oxydans and peroxydans groups, on which the classification of the acetic acid bacteria in the 8th and 9th edition of Bergey's manual is based. 相似文献
274.
Jens Krause Jean-Guy J. Godin 《Ethology : formerly Zeitschrift fur Tierpsychologie》1994,98(2):128-136
Banded killifish (Fundulus diaphanus) were presented individually with a choice of shoaling with either of two stimulus shoals which differed in shoal size, species composition, and fish body size, before and after a simulated avian predator attack. When threatened, test fish preferred to shoal with the larger of two conspecific shoals, but only if members of both stimulus shoals were of the same size class as the test fish. Otherwise, they preferred to shoal with similarly sized fish irrespective of shoal size; threat of predation increased the magnitude of this preference. Furthermore, test fish preferred a shoal of similarly sized shiners (Notemigonus crysoleucas) over larger killifish, when shoal sizes were identical. This indicates that body size plays a key role in shoal choice, overriding the effects of shoal size and species preference. Notwithstanding the above, shoal choice was affected by predator threat only when differences between shoal size or body size of stimulus fish were large. 相似文献
275.
Do Bien-Cuong Dang Thi-Thu Jean-Guy Berrin Dietmar Haltrich To Kim-Anh Jean-Claude Sigoillot Montarop Yamabhai 《Microbial cell factories》2009,8(1):59-12