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151.
Nathalie Griffon Catherine Pilon François Sautel Jean-Charles Schwartz Pierre Sokoloff 《Journal of neurochemistry》1997,68(1):1-9
Abstract: As cerebral neurons express the dopamine D1 receptor positively coupled with adenylyl cyclase, together with the D3 receptor, we have investigated in a heterologous cell expression system the relationships of cyclic AMP with D3 receptor signaling pathways. In NG108-15 cells transfected with the human D3 receptor cDNA, dopamine, quinpirole, and other dopamine receptor agonists inhibited cyclic AMP accumulation induced by forskolin. Quinpirole also increased mitogenesis, assessed by measuring [3 H]thymidine incorporation. This effect was blocked partially by genistein, a tyrosine kinase inhibitor. Forskolin enhanced by 50–75% the quinpirole-induced [3 H]thymidine incorporation. This effect was maximal with 100 n M forskolin, occurred after 6–16 h, was reproduced by cyclic AMP-permeable analogues, and was blocked by a protein kinase A inhibitor. Forskolin increased D3 receptor expression up to 135%, but only after 16 h and at concentrations of >1 µ M . Thus, in this cell line, the D3 receptor uses two distinct signaling pathways: it efficiently inhibits adenylyl cyclase and induces mitogenesis, an effect possibly involving tyrosine phosphorylation. Activation of the cyclic AMP cascade potentiates the D3 receptor-mediated mitogenic response, through phosphorylation by a cyclic AMP-dependent kinase of a yet unidentified component. Hence, transduction of the D3 receptor can involve both opposite and synergistic interactions with cyclic AMP. 相似文献
152.
Summary A protocol was developed for the preparation of Cucumis sativus var Straight 8 protoplasts that incorporates a two-step Ficoll® gradient and results in a high percentage of viable, debris-free protoplasts suitable for the transient expression of foreign genes. Polyethylene glycol and electroporation were compared for their effect on protoplast transfection with commonly used reporter genes. Using a polyethylene glycol method, cucumber protoplasts transfected with a plasmid containing the -glucuronidase gene showed high expression levels, while protoplasts transfected with a plasmid containing the chloramphenicol acetyl transferase gene showed levels of activity that were barely distinguishable from mock-transfected controls. Tomato ringspot virus genomic RNA was also transfected into the protoplasts, and the assembly of viral particles was confirmed. 相似文献
153.
Fran?ois Guerrero Henri Burnet 《European journal of applied physiology and occupational physiology》1995,71(1):87-94
Carotid blood flow was measured in rats by implanted transit-time ultrasonic flowprobes during hyperbaric experiments at up to 70 bar (7 MPa) using an helium-oxygen hyperoxic (partial pressure of O2 = 400 mbar) mixture. Before the hyperbaric experiment, an intracerebroventricular injection of phosphate saline buffered solution (PBS) or aminophylline, an adenosine receptor blocker, in PBS was given. Throughout the hyperbaric experiment carotid blood flow increased with ambient pressure in both PBS, i.e. control, and aminophylline treated rats. The increase in carotid blood flow was significantly attenuated in aminophylline treated rats. Additional experiments showed that the increased carotid blood flow was independent of hyperoxia as well as of temperature. The hypothesis that the hyperbaric dependent increase in carotid blood flow was mediated by brain adenosine receptors and its implication regarding a cerebral vasodilatation are discussed. 相似文献
154.
Richard S. Dodd Franois Fromard Zara A. Rafii Franois Blasco 《Biochemical Systematics and Ecology》1995,23(7-8):859-868
Foliage from 21 red mangroves (Rhizophora mangle, R. racemosa and R. x harrisonii) from different ecogeographic conditions in Gabon, West Africa, was analyzed for epicuticular wax composition using CC, GC and GC-MS. Aliphatic hydrocarbons ranging from C23 to C34 and some triterpenoids were identified. Alkanes were the major constituents (46.7–99.9%), with triterpenoids also accounting for up to 53.3% of the wax extract. High contents of octacosane (27.2%) and lower amounts of nonacosane (14.9%) and hentriacontane (9.8%) distinguished R. mangle from the other two species. R. hizophora x harrisonii was exceptionally rich in nonacosane (45.3%) with moderately high concentrations of hentriacontane (25.4%), whereas R. racemosa was intermediate between the two former species in its content of these three alkanes. Inclusion of the less abundant constituents in principal components analysis provided a good separation of R. x harrisonii, whereas the two other species showed some degree of overlap. Although major alkane patterns can be used to discriminate among the Rhizophora species examined, we do find substantial intra-specific variation that may be attributable to population genetic variation; this was least for R. x harrisonii. The hybrid status of this latter species could not be confirmed from the biochemical analyses carried out. 相似文献
155.
Ghislaine Le Prince Pia Delaere Christiane Fages Thierry Lefrançois Monique Touret Marcelle Salanon Marcienne Tardy 《Neurochemical research》1995,20(7):859-862
Glutamine synthetase (GS), a metabolic marker of the mature astrocyte, was investigated in the temporal neocortex of postmortem brain samples of 8 cases, either not demented or affected by senile dementia of the Alzheimer type. A negative correlation between the GS protein level and the density of both classical A4 deposits and senile plaques was evidenced. Such a correlation for GS underlies a dysfunction of the astroglial metabolism and particularly of the glutamate and ammonia neutralization. Since GS is sensitive to oxidative lesioning, the changes in GS level that were observed, occurring at the posttranslational stage, might reflect oxidative damage and have severe consequences on the pathological cascade of events. 相似文献
156.
M. L. Kottler F. Lorenzo F. Bergametti P. Commerçon C. Souchier R. Counis 《Human genetics》1995,96(4):477-480
We have isolated nine yeast artificial chromosomes (YACs) containing the gene that encodes the human gonadotropin-releasing hormone receptor (GnRH-R) gene by screening the YAC library of the Centre d'Etude du Polymorphisme Humain (Hôpital Saint-Louis, Paris, France) by the use of the polymerase chain reaction. We defined the location of the GnRH-R gene relative to 4q microsatellite markers D4S392 and D4S409. The genetic positions of these markers on chromosome 4 are 76 and 77 cM, respectively. This location was further established by chromosomal in situ hybridization. 相似文献
157.
Suzanne Demczuk Annie Lévy Muriel Aubry Marie-Françoise Croquette Nicole Philip Marguerite Prieur Ursula Sauer Patrice Bouvagnet Guy A. Rouleau Gilles Thomas Alain Aurias 《Human genetics》1995,96(1):9-13
We have determined the parental origin of the deleted chromosome 22 in 29 cases of DiGeorge syndrome (DGS) using a CA-repeat mapping within the commonly deleted region, and in one other case by using a chromosome 22 short arm heteromorphism. The CA-repeat was informative in 21 out of 29 families studied and the deleted chromosome was of maternal origin in 16 cases (72%). When these data are pooled with recent results from the literature, 24 de novo DGS, velo-cardio-facial syndrome (VCFS) and isolated conotruncal cardiac disease deletions are found to be of maternal origin and 8 of paternal origin, yielding a 2 of 8 with a probability level lower than 0.01. These data, and review of the literature on familial DGS/VCFS and isolated conotruncal cardiopathies suggest that there is a strong tendency for the 22q11.2 deletions to be of maternal origin. 相似文献
158.
Narendra Tuteja Ning Wu Huang Doris Skopac Renu Tuteja Sara Hrvatic Jianwen Zhang Sandor Pongor Grard Joseph Christian Faucher Franois Amalric Arturo Falaschi 《Gene》1995,160(2):143-148
The cDNA encoding human DNA helicase IV (HDH IV), a 100-kDa protein which unwinds DNA in the 5′ to 3′ direction with respect to the bound strand, was cloned and sequenced. It was found to be identical to the human cDNA encoding nucleolin, a ubiquitous eukaryotic protein essential for pre-ribosome assembly. HDH IV/nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Phosphorylation of HDH IV/nucleolin by cdc2 kinase and casein kinase II enhanced its unwinding activity in an additive way. The Gly-rich C-terminal domain possesses a limited ATP-dependent duplex-unwinding activity which contributes to the helicase activity of HDH IV/nucleolin. 相似文献
159.
We studied the winter resource selection of muskoxen Ovibos moschatus in the High Arctic using a nested hierarchy of spatial scales 1) population range, 2) travel routes, 3) feeding sites (l e clusters of feeding craters), 4) feeding craters, and 5) diet (I e plant species) We found that, generally, patterns of selection remained consistent across all levels At successively smaller scales, muskoxen selected for higher graminoid abundance and particularly for thinner, softer snow cover, although we did not reject the hypothesis of random travel route selection Muskoxen uncovered forages from beneath the snow cover, by cratering, near the flonstic and nival extremes of availability Selection was consistently biased toward use of water sedge, Carex aquatilis As scale changed, however, muskoxen showed reversals of preference for some other forage species Diet was dominated by C aquatilis and cotton sedge, Eriophorum angustifolium , species characteristic of lowland meadows During spring melt, muskoxen moved to snow-free uplands to feed Dietary quality, as revealed by fecal nitrogen, increased at this time The consistency of the results across scales implied that these local levels of habitat selection occurred within one scaling domain 相似文献
160.
Global scale patterns of fish species richness in rivers 总被引:6,自引:0,他引:6
Explanations of spatial and temporal variation in species richness is a central theme in community ecology Until recently, most research has focused on small-scale phenomena, often emphasizing on local environmental factors and, thus, poorly reflecting large-scale processes that organize species richness In this paper, we analyze variations in species richness of indigeneous freshwater fish on a worldwide scale We show that factors related to species-area and species-energy theories statistically explain most of the variation in freshwater fish species richness across continents Historical events supposed to influence present distributions offish are of little assistence in explaining variations in fish species richness at the global scale Our model, which uses easily measured factors, should also be of practical value to aquatic conservation biology and natural resource management 相似文献