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991.
Vincent Billard Alain Ourry Anne Maillard Maria Garnica Laurent Coquet Thierry Jouenne Florence Cruz José-Maria Garcia-Mina Jean-Claude Yvin Philippe Etienne 《PloS one》2014,9(10)
During the last 40 years, crop breeding has strongly increased yields but has had adverse effects on the content of micronutrients, such as Fe, Mg, Zn and Cu, in edible products despite their sufficient supply in most soils. This suggests that micronutrient remobilization to edible tissues has been negatively selected. As a consequence, the aim of this work was to quantify the remobilization of Cu in leaves of Brassica napus L. during Cu deficiency and to identify the main metabolic processes that were affected so that improvements can be achieved in the future. While Cu deficiency reduced oilseed rape growth by less than 19% compared to control plants, Cu content in old leaves decreased by 61.4%, thus demonstrating a remobilization process between leaves. Cu deficiency also triggered an increase in Cu transporter expression in roots (COPT2) and leaves (HMA1), and more surprisingly, the induction of the MOT1 gene encoding a molybdenum transporter associated with a strong increase in molybdenum (Mo) uptake. Proteomic analysis of leaves revealed 33 proteins differentially regulated by Cu deficiency, among which more than half were located in chloroplasts. Eleven differentially expressed proteins are known to require Cu for their synthesis and/or activity. Enzymes that were located directly upstream or downstream of Cu-dependent enzymes were also differentially expressed. The overall results are then discussed in relation to remobilization of Cu, the interaction between Mo and Cu that occurs through the synthesis pathway of Mo cofactor, and finally their putative regulation within the Calvin cycle and the chloroplastic electron transport chain. 相似文献
992.
Eukaryotes use sexual recombination to achieve innovation and adaptation to a changing environment, a mechanism that is exceptional in Leishmania. It is postulated that asexual mechanisms contribute efficiently to parasite fitness and that sexual recombination would not be necessary for the production of a large repertoire of genotypes. The model discussed in this review used a major Leishmania glycoprotein, gp63, which is involved in host-parasite relationships. Mitotic recombination, which occurs between and within tandem repeats, amplifies genes and generates genotypic diversity. The resulting variation in the protein sequence is concentrated in surface domains, in regions spanning T-cell epitopes and B-cell epitopes and might allow immune escape. 相似文献
993.
Ba S Willems A de Lajudie P Roche P Jeder H Quatrini P Neyra M Ferro M Promé JC Gillis M Boivin-Masson C Lorquin J 《Systematic and applied microbiology》2002,25(1):130-145
A collection of rhizobia isolated from Acacia tortilis subsp. raddiana from various sites in the North and South of Sahara was analyzed for their diversity at both taxonomic and symbiotic levels. On the basis of whole cell protein (SDS-PAGE) and 16S rDNA sequence analysis, most of the strains were found to belong to the Sinorhizobium and Mesorhizobium genera where they may represent several different genospecies. Despite their chromosomal diversity, most A. tortilis Mesorhizobium and Sinorhizobium symbionts exhibited very similar symbiotic characters. Nodulation tests showed that the strains belong to the Acacia-Leucaena-Prosopis nodulation group, although mainly forming non-fixing nodules on species other than A. tortilis. Most of the strains tested responded similarly to flavonoid nod gene inducers, as estimated by using heterologous nodA-lacZ fusions. Thin layer chromatography analysis of the Nod factors synthesized by overproducing strains showed that most of the strains exhibited similar profiles. The structures of Nod factors produced by four different Sinorhizobium sp. strains were determined and found to be similar to other Acacia-Prosopis-Leucaena nodulating rhizobia of the Sinorhizobium-Mesorhizobium-Rhizobium branch. They are chitopentamers, N-methylated and N-acylated by common fatty acids at the terminal non reducing sugar. The molecules can also be 6-O sulfated at the reducing end and carbamoylated at the non reducing end. The phylogenetic analysis of available NodA sequences, including new sequences from A. tortilis strains, confirmed the clustering of the NodA sequences of members of the Acacia-Prosopis-Leucaena nodulation group. 相似文献
994.
Louis?HautierEmail author Gilles?San Martin Pierre?Callier Jean-Christophe?de?Biseau Jean-Claude?Grégoire 《Biological invasions》2011,13(8):1805-1814
Over a period of less than 5 years, Belgium was thoroughly invaded by the multicolored Asian coccinellid, Harmonia axyridis. At the same time, a decline of some native coccinellid species was observed in tree habitats. One hypothesis about the cause
of this decline was that it might have been due to intraguild predation (IGP) by H. axyridis. In natural conditions, IGP between coccinellids can be tracked by using defensive compounds. Exogenous alkaloids in H. axyridis were therefore examined by gas chromatography-mass spectrometry (GC–MS), using individuals sampled from lime trees that were
also occupied by other species of coccinellids. Harmonia axyridis was the dominant species at all life stages, in terms of both numbers of sites occupied and local abundance. The GC–MS analysis
of H. axyridis larvae revealed traces of exogenous alkaloids from 19 of the 20 sites and, in nine of those 19 sites, more than 30% of the
larvae analyzed contained exogenous alkaloids. Three alkaloids were detected: adaline from Adalia spp., calvine from Calvia spp. and propyleine from Propylea quatuordecimpunctata. Predation by H. axyridis on two different coccinellid species was also detected in the same larva, reinforcing the status of H. axyridis as a top predator. A generalized linear model indicated that IGP frequency was positively influenced by two variables: the
abundance of extraguild and intraguild prey; and the interaction between these two variables. Our results therefore support
the hypothesis that IGP by H. axyridis on native coccinellids in tree habitats has led to the decline of several of these species. 相似文献
995.
de Barsy M Jamet A Filopon D Nicolas C Laloux G Rual JF Muller A Twizere JC Nkengfac B Vandenhaute J Hill DE Salcedo SP Gorvel JP Letesson JJ De Bolle X 《Cellular microbiology》2011,13(7):1044-1058
Bacteria of the Brucella genus are facultative intracellular class III pathogens. These bacteria are able to control the intracellular trafficking of their vacuole, presumably by the use of yet unknown translocated effectors. To identify such effectors, we used a high-throughput yeast two-hybrid screen to identify interactions between putative human phagosomal proteins and predicted Brucella spp. proteins. We identified a specific interaction between the human small GTPase Rab2 and a Brucella spp. protein named RicA. This interaction was confirmed by GST-pull-down with the GDP-bound form of Rab2. A TEM-β-lactamase-RicA fusion was translocated from Brucella abortus to RAW264.7 macrophages during infection. This translocation was not detectable in a strain deleted for the virB operon, coding for the type IV secretion system. However, RicA secretion in a bacteriological culture was still observed in a ΔvirB mutant. In HeLa cells, a ΔricA mutant recruits less GTP-locked myc-Rab2 on its Brucella-containing vacuoles, compared with the wild-type strain. We observed altered kinetics of intracellular trafficking and faster proliferation of the B. abortusΔricA mutant in HeLa cells, compared with the wild-type control. Altogether, the data reported here suggest RicA as the first reported effector with a proposed function for B. abortus. 相似文献
996.
Background
The estimation of individual ancestry from genetic data has become essential to applied population genetics and genetic epidemiology. Software programs for calculating ancestry estimates have become essential tools in the geneticist's analytic arsenal.Results
Here we describe four enhancements to ADMIXTURE, a high-performance tool for estimating individual ancestries and population allele frequencies from SNP (single nucleotide polymorphism) data. First, ADMIXTURE can be used to estimate the number of underlying populations through cross-validation. Second, individuals of known ancestry can be exploited in supervised learning to yield more precise ancestry estimates. Third, by penalizing small admixture coefficients for each individual, one can encourage model parsimony, often yielding more interpretable results for small datasets or datasets with large numbers of ancestral populations. Finally, by exploiting multiple processors, large datasets can be analyzed even more rapidly.Conclusions
The enhancements we have described make ADMIXTURE a more accurate, efficient, and versatile tool for ancestry estimation. 相似文献997.
Nazarko VY Nazarko TY Farré JC Stasyk OV Warnecke D Ulaszewski S Cregg JM Sibirny AA Subramani S 《Autophagy》2011,7(4):375-385
Autophagy-related (Atg) pathways deliver cytosol and organelles to the vacuole in double-membrane vesicles called autophagosomes, which are formed at the phagophore assembly site (PAS), where most of the core Atg proteins assemble. Atg28 is a component of the core autophagic machinery partially required for all Atg pathways in Pichia pastoris. This coiled-coil protein interacts with Atg17 and is essential for micropexophagy. However, the role of Atg28 in micropexophagy was unknown. We used the yeast two-hybrid system to search for Atg28 interaction partners from P. pastoris and identified a new Atg protein, named Atg35. The atg35Δ mutant was not affected in macropexophagy, cytoplasm-to-vacuole targeting or general autophagy. However, both Atg28 and Atg35 were required for micropexophagy and for the formation of the micropexophagic apparatus (MIPA). This requirement correlated with a stronger expression of both proteins on methanol and glucose. Atg28 mediated the interaction of Atg35 with Atg17. Trafficking of overexpressed Atg17 from the peripheral ER to the nuclear envelope was required to organize a peri-nuclear structure (PNS), the site of Atg35 colocalization during micropexophagy. In summary, Atg35 is a new Atg protein that relocates to the PNS and specifically regulates MIPA formation during micropexophagy.Key words: Atg protein, peroxisome, micropexophagy, MIPA, nucleus 相似文献
998.
Vivian de Buffrénil Yannicke Dauphin Jean-Claude Rage Jean-Yves Sire 《Comptes Rendus Palevol》2011,10(5-6):427-437
The Glyptosaurinae, a fossil clade of anguid lizards, have robust osteoderms, with a granular ornamentation consisting of tubercles. In this study, the structural and histological features of these osteoderms are described in order to reconstruct their developmental pattern and further document the possible homology that could exist between vertebrate integumentary skeletons. Glyptosaurine osteoderms display a diploe architecture and an unusually complex structure that includes four tissue types: an intensely remodeled core of woven-fibered bone, a thick basal layer of lamellar bone, a peripheral ring exhibiting histological features intermediate between these two tissues and containing dense bundles of long Sharpey fibers, and a superficial layer made of a monorefringent, acellular and highly mineralized material, different from bone, and comparable in many respects to hypermineralized tissues such as ganoine, enameloids and enamel. We call this tissue osteodermine. The growth pattern of glyptosaurine osteoderms is likely to have involved first metaplasia, at an early developmental stage, then appositional growth due to osteoblast activity. The superficial layer that is well developed at the tubercle level must have resulted from epidermal and dermal contributions, a conclusion that would support previous hypotheses on the role of epidermal-dermal interactions in the formation of squamate osteoderms. 相似文献
999.
1000.
Serino M Waget A Marsollier N Masseboeuf M Payros G Kabani C Denom J Lacombe A Thiers JC Negre-Salvayre A Luquet S Burcelin R Cruciani-Guglielmacci C Magnan C 《PloS one》2011,6(6):e21184