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581.
Phosphorus metabolites and intracellular pH have been examined in the slime mold Dictyostelium discoideum by non-destructive 31P-NMR measurements. In a spectrum from a suspension of aerobic amoebae, the major peaks are inorganic phosphate, nucleotide di- and triphosphates. In the corresponding perchloric acid extract, resonances originating from purine and pyrimidine nucleotides are resolved. Adenine nucleotides are the most abundant components, but the other nucleotides are present in significant amounts. In a spectrum from intact spores in a dormant state, only inorganic phosphate and polyphosphates are detected and nucleotides are no longer present in large amounts.Of particular importance is the ability to observe separately in aerobic amoebae the resonance of inorganic phosphate localized in two different cell compartments: the cytosol and the mitochondria. The cytosolic pH and mitochondrial pH have been measured as 6.7 and 7.7, respectively, on the basis of intracellular inorganic phosphate chemical shifts. They are essentially unaffected over a large range of external pH and they are not modified transiently or permanently during the initiation of the developmental program of the organism. A weak acid, such as propionate, which modifies the progression of differentiation by favoring prestalk cells, perturbs intracellular pH gradients by selectively decreasing mitochondrial pH without any effect on cytosolic pH. 相似文献
582.
Tanja Pintsch Michel Satre Gérard Klein Jean-Baptiste Martin Stephan C Schuster 《BMC cell biology》2001,2(1):9-15
Background
Dictyostelium cells exhibit an unusual response to hyperosmolarity that is distinct from the response in other organisms investigated: instead of accumulating compatible osmolytes as it has been described for a wide range of organisms, Dictyostelium cells rearrange their cytoskeleton and thereby build up a rigid network which is believed to constitute the major osmoprotective mechanism in this organism. To gain more insight into the osmoregulation of this amoeba, we investigated physiological processes affected under hyperosmotic conditions in Dictyostelium. 相似文献583.
Oscar Mendoza Anne Bourdoncle Jean-Baptiste Boulé Robert M. Brosh Jr Jean-Louis Mergny 《Nucleic acids research》2016,44(5):1989-2006
Guanine-rich DNA strands can fold in vitro into non-canonical DNA structures called G-quadruplexes. These structures may be very stable under physiological conditions. Evidence suggests that G-quadruplex structures may act as ‘knots’ within genomic DNA, and it has been hypothesized that proteins may have evolved to remove these structures. The first indication of how G-quadruplex structures could be unfolded enzymatically came in the late 1990s with reports that some well-known duplex DNA helicases resolved these structures in vitro. Since then, the number of studies reporting G-quadruplex DNA unfolding by helicase enzymes has rapidly increased. The present review aims to present a general overview of the helicase/G-quadruplex field. 相似文献
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586.
Jean-Baptiste Leca Noëlle Gunst Michael A. Huffman 《Primates; journal of primatology》2010,51(1):13-22
We report the first case of dental flossing behavior by a Japanese macaque. We used cross-sectional data to assess the presence
of this novel tool-use behavior at the group-level. Although this behavior was performed frequently by a central middle-ranking
middle-aged female during her grooming interactions, and appeared at least four years ago, it remained idiosyncratic to its
innovator, and until now has not spread to other group members. We examined the factors that may have favored this innovation,
including the environmental context, the individual characteristics of the innovator, and the structural and functional aspects
of the behavior. Group size, kinship, and dominance are socio-demographic factors that are likely to limit the opportunities
for any group member to observe the innovator, and thus constrain the diffusion of this potential candidate as a new behavioral
tradition. This is one of the rare studies to document the spontaneous appearance of tool-use behavior in primates under natural
conditions. Identifying the determinants of innovations and the constraints on their diffusion within social groups of non-human
primates is of special interest to understanding cultural evolution. 相似文献
587.
Jean-Baptiste Leca Noëlle Gunst Matthew Gardiner I. Nengah Wandia 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2021,376(1819)
The token exchange paradigm shows that monkeys and great apes are able to use objects as symbolic tools to request specific food rewards. Such studies provide insights into the cognitive underpinnings of economic behaviour in non-human primates. However, the ecological validity of these laboratory-based experimental situations tends to be limited. Our field research aims to address the need for a more ecologically valid primate model of trading systems in humans. Around the Uluwatu Temple in Bali, Indonesia, a large free-ranging population of long-tailed macaques spontaneously and routinely engage in token-mediated bartering interactions with humans. These interactions occur in two phases: after stealing inedible and more or less valuable objects from humans, the macaques appear to use them as tokens, by returning them to humans in exchange for food. Our field observational and experimental data showed (i) age differences in robbing/bartering success, indicative of experiential learning, and (ii) clear behavioural associations between value-based token possession and quantity or quality of food rewards rejected and accepted by subadult and adult monkeys, suggestive of robbing/bartering payoff maximization and economic decision-making. This population-specific, prevalent, cross-generational, learned and socially influenced practice may be the first example of a culturally maintained token economy in free-ranging animals.This article is part of the theme issue ‘Existence and prevalence of economic behaviours among non-human primates''. 相似文献