全文获取类型
收费全文 | 55311篇 |
免费 | 5230篇 |
国内免费 | 33篇 |
出版年
2022年 | 381篇 |
2021年 | 740篇 |
2020年 | 539篇 |
2019年 | 708篇 |
2018年 | 878篇 |
2017年 | 806篇 |
2016年 | 1361篇 |
2015年 | 2271篇 |
2014年 | 2393篇 |
2013年 | 3171篇 |
2012年 | 3720篇 |
2011年 | 3723篇 |
2010年 | 2471篇 |
2009年 | 2122篇 |
2008年 | 3097篇 |
2007年 | 3092篇 |
2006年 | 2850篇 |
2005年 | 2957篇 |
2004年 | 2877篇 |
2003年 | 2814篇 |
2002年 | 2761篇 |
2001年 | 654篇 |
2000年 | 545篇 |
1999年 | 694篇 |
1998年 | 804篇 |
1997年 | 599篇 |
1996年 | 555篇 |
1995年 | 535篇 |
1994年 | 475篇 |
1993年 | 439篇 |
1992年 | 518篇 |
1991年 | 438篇 |
1990年 | 376篇 |
1989年 | 388篇 |
1988年 | 360篇 |
1987年 | 341篇 |
1986年 | 319篇 |
1985年 | 407篇 |
1984年 | 438篇 |
1983年 | 350篇 |
1982年 | 463篇 |
1981年 | 404篇 |
1980年 | 352篇 |
1979年 | 297篇 |
1978年 | 330篇 |
1977年 | 297篇 |
1976年 | 273篇 |
1975年 | 224篇 |
1974年 | 276篇 |
1973年 | 269篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
102.
Véronique Lavoie Anne‐Elen Kernaleguen Guy Charron Nada Farhat Mariève Cossette Aida M. Mamarbachi Bruce G. Allen Eric Rhéaume Jean‐Claude Tardif 《Obesity (Silver Spring, Md.)》2011,19(4):722-728
Adiponectin is an adipokine whose plasma levels are inversely correlated to metabolic syndrome components. Adiponectin protects against atherosclerosis and decreases risks in myocardial infarction. Endothelial progenitor cells (EPCs) are a heterogeneous population of circulating cells involved in vascular repair and neovascularization. EPCs number is reduced in patients with cardiovascular disease. We hypothesize that the positive effects of adiponectin against atherosclerosis are explained in part by its interactions with EPCs. Cells were obtained from healthy volunteers' blood by mononuclear cell isolation and plating on collagen‐coated dishes. Three sub‐populations of EPCs were identified and characterized using flow cytometry. EPCs' expression of adiponectin receptors, AdipoR1, and AdipoR2 was evaluated by quantitative PCR. The effects of recombinant adiponectin on EPCs' susceptibility to apoptosis were assessed. Finally, expression of neutrophil elastase by EPCs and activity of this enzyme on adiponectin processing were assessed. Quantitative PCR analysis of EPCs mRNAs showed that AdipoR1 mRNA is expressed at higher levels than AdipoR2. Expression of AdipoR1 protein was confirmed by western blot. Adiponectin significantly increased survival of two sub‐populations of EPCs in conditions of serum deprivation. Such effect could not be demonstrated in the third EPCs sub‐population. We also demonstrated that EPCs, particularly one sub‐population, express neutrophil elastase. Neutrophil elastase activity was confirmed in EPCs' conditioned media. Adiponectin protects some EPCs sub‐populations against apoptosis and therefore could modulate EPCs ability to induce repair of vascular damage. Neutrophil elastase activity of EPCs could locally modulate adiponectin activity by its involvement in the generation of the globular form of adiponectin. 相似文献
103.
Effects of applications of municipal compost on uptake of copper,zinc and boron by garden vegetables
Summary Municipal compost applied to soils in plot experiments with cabbages, lettuces, beans, potatoes and peas in three successive years (25–100 tonnes per hectare) has produced significant enhancement of available soil Cu, Zn and B in each year. Associated significant increases in uptake were as follows: Cu, Zn and B with lettuces and beans, Zn and B with potatoes and B with peas. No significant increases in uptake of any of these elements was obtained in two experiments with cabbages.Compost treatment was associated with severe phytotoxic effects in the experiment with beans and with yield responses in the experiments with peas and potatoes. re]19721116 相似文献
104.
105.
106.
107.
108.
109.
Azospirillum strains isolated from the roots and rhizosphere of some plants growing in West Bengal were subjected to qualitative and quantitative
evaluation for poly-3-hydroxybutyrate (PHB) production. Out of the total 49 isolates, 13 (26%) were confirmed as PHB producers
according to staining and chemical assay methods. The majority of these strains belonged toAzospirillum brasilense butA. amazonense andA. lipoferum were also present. When grown in the presence of NH4Cl in the medium, the PHB content of the strains ranged from 1 to 14% of cell dry mass. The identity of the PHB extracted
fromAzospirillum strain 24P-N-72 was confirmed by the characteristic UV and IR absorption peaks at 235 nm and 1730 cm−1, respectively. 相似文献
110.
R A Seder M Plaut S Barbieri J Urban F D Finkelman W E Paul 《Journal of immunology (Baltimore, Md. : 1950)》1991,147(3):903-909
Non-B, non-T cells from spleen and bone marrow cells produce IL-4 in response to cross-linkage of high affinity receptors for Fc epsilon R or Fc gamma RII, and to treatment with calcium ionophores. Cells bearing high affinity Fc epsilon R constituted 1 to 2% of non-B, non-T cells of spleen and of total bone marrow cells from naive donors. In mice whose immune systems had been polyclonally activated by injection with anti-IgD antibodies or had been infected with Nippostrongylus brasiliensis larvae, the frequency of Fc epsilon R+ cells in splenic non-B, non-T cells was also 1 to 2% but in bone marrow from anti-IgD-injected mice donors the frequency was approximately 5%. Cell sorting experiments revealed that all of the capacity to produce IL-4 in response to immobilized IgE or IgG2a or to ionomycin was found in the Fc epsilon R+ fraction. Among the Fc epsilon R+ spleen cells from naive donors, the frequency of IL-4-producing cells was 1/20 to 1/40 whereas in mice that had been injected with anti-IgD or infected with N. brasiliensis, the frequency of IL-4 producing cells in the Fc epsilon R+ population was approximately 1/5. 相似文献