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961.
962.
Eugene Diatloff Rémi Peyronnet Jean Colcombet Sébastien Thomine Hélène Barbier-Brygoo Jean-Marie Frachisse 《Plant signaling & behavior》2010,5(11):1347-1352
Plant genomes code for channels involved in the transport of cations, anions and uncharged molecules through membranes. Although the molecular identity of channels for cations and uncharged molecules has progressed rapidly in the recent years, the molecular identity of anion channels has lagged behind. Electrophysiological studies have identified S-type (slow) and R-type (rapid) anion channels. In this brief review, we summarize the proposed functions of the R-type anion channels which, like the S-type, were first characterized by electrophysiology over 20 years ago, but unlike the S-type, have still yet to be cloned. We show that the R-type channel can play multiple roles.Key words: R-type anion channel, nitrate, sulphate, guard cell, action potentialAnion channels play a central role in signal transduction, nutrient transport and cell turgor regulation.1 By far, their function was particularly well investigated in the guard cells of stomata using a combination of electrophysiological, pharmacological and genetic tools. In this system, anion channel activation was shown to be one of the limiting steps in the loss of cell turgor leading to stomatal closure.2 In algal cells, anion channels were shown to contribute to membrane excitability through the generation of action potential.1,3With the burst of molecular biology in the nineties, the genes coding for plant ion channels started to be unveiled. The first channel gene to be cloned in plant was the shaker-like potassium channel identified in a yeast functional expression screen.4,5 More than ten years later, TaALMT1 and AtCLCa were characterized as the first members of two important anion channel families.6,7 This growing group of newly identified channels, accounting for electrophysiological activity described long ago, includes the MSLs anion selective mechanosensitive channels.8 Recently, the well known S-type channel has been finally recognized to be encoded by members of the SLAC1 (and other SLAH) family (Slow Anion Channel-Associated 1).9 In agreement with electrophysiological data,10–13 it requires phosphorylation by a Protein Kinase in order to be functional.14,15 In contrast, the molecular identity of the R-type anion channel remains unknown. Therefore, this candidate, which has been functionally known since twenty years, remains the next challenge for plant channel physiologists. 相似文献
963.
964.
Lyme disease risk is increasing in the United States due in part to the spread of blacklegged ticks Ixodes
scapularis, the principal vector of the spirochetal pathogen Borrelia
burgdorferi. A 5-year study was undertaken to investigate hypothesized coinvasion of I. scapularis and B. burgdorferi in Lower Michigan. We tracked the spatial and temporal dynamics of the tick and spirochete using mammal, bird, and vegetation
drag sampling at eight field sites along coastal and inland transects originating in a zone of recent I.
scapularis establishment. We document northward invasion of these ticks along Michigan’s west coast during the study period; this pattern
was most evident in ticks removed from rodents. B.
burgdorferi infection prevalences in I.
scapularis sampled from vegetation in the invasion zone were 9.3% and 36.6% in nymphs and adults, respectively, with the majority of
infection (95.1%) found at the most endemic site. There was no evidence of I. scapularis invasion along the inland transect; however, low-prevalence B.
burgdorferi infection was detected in other tick species and in wildlife at inland sites, and at northern coastal sites in years before
the arrival of I.
scapularis. These infections suggest that cryptic B.
burgdorferi transmission by other vector-competent tick species is occurring in the absence of I.
scapularis. Other Borrelia spirochetes, including those that group with B. miyamotoi and B. andersonii, were present at a low prevalence within invading ticks and local wildlife. Reports of Lyme disease have increased significantly
in the invasion zone in recent years. This rapid blacklegged tick invasion—measurable within 5 years—in combination with cryptic
pathogen maintenance suggests a complex ecology of Lyme disease emergence in which wildlife sentinels can provide an early
warning of disease emergence. 相似文献
965.
TreeSNPs: a laboratory information management system (LIMS) dedicated to SNP discovery in trees 总被引:1,自引:0,他引:1
Sébastien Clément Joël Fillon Jean Bousquet Jean Beaulieu 《Tree Genetics & Genomes》2010,6(3):435-438
TreeSNPs is a laboratory information management system with a user-friendly Web interface for the gathering and display of
data generated through medium- to large-scale single nucleotide polymorphism (SNP) identification by resequencing, in the
context of whole genome association, outlier detection, quantitative trait locus, and genetic mapping studies. Its multi-gene
approach allows the tracking of all the steps from candidate gene classification and prioritization to SNP discovery. The
system, source code, and documentation are available for download at . An online demo version can also be accessed from the above link. 相似文献
966.
Jean‐Charles Blouzard Pedro M. Coutinho Henri‐Pierre Fierobe Bernard Henrissat Sabrina Lignon Chantal Tardif Sandrine Pagès Pascale de Philip 《Proteomics》2010,10(3):541-554
Clostridium cellulolyticum is a model mesophilic anaerobic bacterium that efficiently degrades plant cell walls. The recent genome release offers the opportunity to analyse its complete degradation system. A total of 148 putative carbohydrate‐active enzymes were identified, and their modular structures and activities were predicted. Among them, 62 dockerin‐containing proteins bear catalytic modules from numerous carbohydrate‐active enzymes' families and whose diversity reflects the chemical and structural complexity of the plant carbohydrate. The composition of the cellulosomes produced by C. cellulolyticum upon growth on different substrates (cellulose, xylan, and wheat straw) was investigated by LC MS/MS. The majority of the proteins encoded by the cip‐cel operon, essential for cellulose degradation, were detected in all cellulosome preparations. In the presence of wheat straw, the natural and most complex of the substrates studied, additional proteins predicted to be involved in hemicellulose degradation were produced. A 32‐kb gene cluster encodes the majority of these proteins, all harbouring carbohydrate‐binding module 6 or carbohydrate‐binding module 22 xylan‐binding modules along dockerins. This newly identified xyl‐doc gene cluster, specialised in hemicellulose degradation, comes in addition of the cip‐cel operon for plant cell wall degradation. Hydrolysis efficiencies determined on the different substrates corroborates the finding that cellulosome composition is adapted to the growth substrate. 相似文献
967.
Thomas C. Durand Kjell Sergeant Sébastien Planchon Sabine Carpin Philippe Label Domenico Morabito Jean‐François Hausman Jenny Renaut 《Proteomics》2010,10(3):349-368
The comprehension of metal homeostasis in plants requires the identification of molecular markers linked to stress tolerance. Proteomic changes in leaves and cambial zone of Populus tremula×P. alba (717‐1B4 genotype) were analyzed after 61 days of exposure to cadmium (Cd) 360 mg/kg soil dry weight in pot‐soil cultures. The treatment led to an acute Cd stress with a reduction of growth and photosynthesis. Cd stress induced changes in the display of 120 spots for leaf tissue and 153 spots for the cambial zone. It involved a reduced photosynthesis, resulting in a profound reorganisation of carbon and carbohydrate metabolisms in both tissues. Cambial cells underwent stress from the Cd actually present inside the tissue but also a deprivation of photosynthates caused by leaf stress. An important tissue specificity of the response was observed, according to the differences in cell structures and functions. 相似文献
968.
Jean Peccoud Jean-Christophe Simon Carol von Dohlen Armelle Coeur d’acier Manuel Plantegenest Flavie Vanlerberghe-Masutti Emmanuelle Jousselin 《Comptes rendus biologies》2010,333(6-7):474-487
Aphids are intimately linked with their host plants that constitute their only food resource and habitat, and thus impose considerable selective pressure on their evolution. It is therefore commonly assumed that host plants have greatly influenced the diversification of aphids. Here, we review what is known about the role of host plant association on aphid speciation by examining both macroevolutionary and population-level studies. Phylogenetic studies conducted at different taxonomic levels show that, as in many phytophagous insect groups, the radiation of angiosperms has probably favoured the major Tertiary diversification of aphids. These studies also highlight many aphid lineages constrained to sets of related host plants, suggesting strong evolutionary commitment in aphids’ host plant choice, but they fail to document cospeciation events between aphid and host lineages. Instead, phylogenies of several aphid genera reveal that divergence events are often accompanied by host shifts, and suggest, without constituting a formal demonstration, that aphid speciation could be a consequence of adaptation to new hosts. Experimental and field studies below the species level support reproductive isolation between host races as partly due to divergent selection by their host plants. Selected traits are mainly feeding performances and life cycle adaptations to plant phenology. Combined with behavioural preference for favourable host species, these divergent adaptations can induce pre- and post-zygotic barriers between host-specialized aphid populations. However, the hypothesis of host-driven speciation is seldom tested formally and must be weighed against overlooked explanations involving geographic isolation and non-ecological reproductive barriers in the process of speciation. 相似文献
969.
970.
Paul Guilloteau Véronique Romé Luc Delaby François Mendy Loic Roger Jean Alain Chayvialle 《Regulatory peptides》2010,159(1-3):129-136
The aim of this work was to study, in vivo, the effect of the ingestion of not glycosylated caseinomacropeptide (CMP) on gastric secretion. In Experiments #1 and #2, 7 calves fitted with a gastric pouch received either a diet without CMP (C diet) or C diet in which CMP was introduced (equal to and 5 folds that of CMP quantity contained in cow milk, diets CMP1 and CMP5, respectively). In Experiment #3, 2 calves (with gastric pouch) were fed C diet followed by an “iv perfusion” of CMP. In Experiment #4, 25 calves fed either C, CMP1 or CMP5 diets were fitted with a blood catheter for sample collections. The quantities of daily gastric secretions seemed few modified by CMP ingestion but the profile of these secretions was changed along the day. The most important result is that CMP can inhibit gastric secretions (mainly hydrochloric acid) stimulated by the meal, but there was no dose-dependent response. No similar observations were obtained after perfusion of CMP in jugular vein. CMP was not detected in blood. Results obtained in our experiments are not in favor of its significant intestinal absorption. Gastrin, somatostatin and VIP could be implicated in the mechanisms of regulation. 相似文献