全文获取类型
收费全文 | 42716篇 |
免费 | 16683篇 |
国内免费 | 8篇 |
出版年
2023年 | 87篇 |
2022年 | 225篇 |
2021年 | 646篇 |
2020年 | 2348篇 |
2019年 | 3902篇 |
2018年 | 4037篇 |
2017年 | 4302篇 |
2016年 | 4439篇 |
2015年 | 4687篇 |
2014年 | 4348篇 |
2013年 | 4999篇 |
2012年 | 2884篇 |
2011年 | 2526篇 |
2010年 | 3750篇 |
2009年 | 2432篇 |
2008年 | 1582篇 |
2007年 | 1175篇 |
2006年 | 1103篇 |
2005年 | 1214篇 |
2004年 | 1147篇 |
2003年 | 1162篇 |
2002年 | 1139篇 |
2001年 | 413篇 |
2000年 | 316篇 |
1999年 | 343篇 |
1998年 | 273篇 |
1997年 | 203篇 |
1996年 | 209篇 |
1995年 | 193篇 |
1994年 | 172篇 |
1993年 | 142篇 |
1992年 | 181篇 |
1991年 | 134篇 |
1990年 | 133篇 |
1989年 | 133篇 |
1988年 | 106篇 |
1987年 | 120篇 |
1986年 | 115篇 |
1985年 | 115篇 |
1984年 | 142篇 |
1983年 | 113篇 |
1982年 | 147篇 |
1981年 | 148篇 |
1980年 | 125篇 |
1979年 | 116篇 |
1978年 | 113篇 |
1977年 | 101篇 |
1976年 | 108篇 |
1974年 | 88篇 |
1973年 | 83篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
Javier Murciano‐Calles Andrea Coello Ana Cmara‐Artigas Jose C. Martinez 《Journal of molecular recognition : JMR》2020,33(4)
N‐Methyl‐D‐aspartate (NMDA) receptors are key components in synaptic communication and are highly relevant in central nervous disorders, where they trigger excessive calcium entry into the neuronal cells causing harmful overproduction of nitric oxide by the neuronal nitric oxide synthase (nNOS) protein. Remarkably, NMDA receptor activation is aided by a second protein, postsynaptic density of 95 kDa (PSD95), forming the ternary protein complex NMDA/PSD95/nNOS. To minimize the potential side effects derived from blocking this ternary complex or either of its protein components, a promising approach points to the disruption of the PSD‐95/nNOS interaction which is mediated by a PDZ/PDZ domain complex. Since the rational development of molecules targeting such protein‐protein interaction relies on energetic and structural information herein, we include a thermodynamic and structural analysis of the PSD95‐PDZ2/nNOS‐PDZ. Two energetically relevant events are structurally linked to a “two‐faced” or two areas of recognition between both domains. First, the assembly of a four‐stranded antiparallel β‐sheet between the β hairpins of nNOS and of PSD95‐PDZ2, mainly enthalpic in nature, contributes 80% to the affinity. Second, binding is entropically reinforced by the hydrophobic interaction between side chains of the same nNOS β‐hairpin with the side chains of α2‐helix at the binding site of PSD95‐PDZ2, contributing the remaining 20% of the total affinity. These results suggest strategies for the future rational design of molecules able to disrupt this complex and constitute the first exhaustive thermodynamic analysis of a PDZ/PDZ interaction. 相似文献
992.
Benildo S. Cavada Mayara T. L. Silva Vinicius J. S. Osterne Vanir R. Pinto‐Junior Claudia F. Lossio Juliana C. Madeira Maria G. Pereira Rodrigo B. Leal Wandemberg P. Ferreira Kyria S. Nascimento Ana M. S. Assreuy 《Journal of molecular recognition : JMR》2020,33(11)
Lectins are a group of proteins of non‐immune origin recognized for their ability to bind reversibly to carbohydrates. Researchers have been intrigued by oligosaccharides and glycoconjugates for their involvement as mediators of complex cellular events and then many biotechnological applications of lectins are based on glycocode decoding and their activities. Here, we report a structural and biological study of a ConA‐like mannose/glucose‐specific lectin from Canavalia bonariensis seeds, CaBo. More specifically, we evaluate the binding of CaBo with α‐methyl‐D‐mannoside (MMA) and mannose‐1,3‐α‐D‐mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation. A virtual screening was also carried out to cover a larger number of possible bindings of CaBo. In silico analysis demonstrated the stability of CaBo interaction with mannose‐type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release. These results confirm the ability of CaBo to interact with hybrid and high‐mannose N‐glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors. 相似文献
993.
994.
995.
996.
997.
998.
999.
1000.
Mateusz Kurcinski Aleksandra Badaczewska‐Dawid Michal Kolinski Andrzej Kolinski Sebastian Kmiecik 《Protein science : a publication of the Protein Society》2020,29(1):211-222
Molecular docking of peptides to proteins can be a useful tool in the exploration of the possible peptide binding sites and poses. CABS‐dock is a method for protein–peptide docking that features significant conformational flexibility of both the peptide and the protein molecules during the peptide search for a binding site. The CABS‐dock has been made available as a web server and a standalone package. The web server is an easy to use tool with a simple web interface. The standalone package is a command‐line program dedicated to professional users. It offers a number of advanced features, analysis tools and support for large‐sized systems. In this article, we outline the current status of the CABS‐dock method, its recent developments, applications, and challenges ahead. 相似文献