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81.
Role of Thidiazuron (TDZ) in inducing adventitious organogenesis in Pongamia was studied. TDZ at different concentrations (0, 0.45, 2.27, 4.54, 6.71, 9.08, 11.35, 13.12 and 22.71 μM) were used for induction
of caulogenic bud formation in deembryonated cotyledon explants. Each cotyledon was cut into three segments and identified
as proximal, middle and distal. Duration of TDZ exposure, influence of the segment and orientation of the explant were studied.
TDZ at 11.35 μM concentration was optimum for the induction of shoots and rapid elongation. Shoots induced at higher concentration
elongated after several passages in growth regulator free medium, thereby extending the period of differentiation. Exposure
of the explant for 20 days yielded more number of buds than 10 days. Proximal segment of the cotyledon was more responsive.
Contact of abaxial surface in the medium was more effective and generated more buds than the adaxial side. Buds differentiated
and elongated on transfer to MS basal medium for 8–12 passages of 15 days each. Rooting and elongation of shoots was achieved
in charcoal supplemented half-strength MS medium. Rooted plantlets survived on transfer to sand soil mixture. The plants were
hardened and transferred to green house. This is the first report on in vitro regeneration of Pongamia pinnata via adventitious organogenesis using TDZ. This protocol may find application in studies in genetic transformation, isolation
of somaclonal variants and in induction of mutants. It also provides a system to study the inhibitory role of TDZ on shoot
differentiation. 相似文献
82.
Background
Evolution of parasite traits is inextricably linked to their hosts. For instance one common definition of parasite virulence is the reduction in host fitness due to infection. Thus, traits of infection must be viewed in both protagonists and may be under shared genetic and physiological control. We investigated these questions on the oomycete Hyaloperonospora arabidopsis (= parasitica), a natural pathogen of the Brassicaceae Arabidopsis thaliana. 相似文献83.
Jill S. Marty James P. Higham Elizabeth L. Gadsby Caroline Ross 《International journal of primatology》2009,30(6):807-823
Sexual selection has driven the evolution and elaboration of a wide variety of displays and ornaments in male nonhuman primates,
including capes, cheek flanges, and sexual coloration. Among the most sexually dimorphic of all primates is the drill (Mandrillus leucophaeus), the males of which can be 3 times the mass of females, possess large canines, and exhibit extremely bright sexual skin
coloration. However, the function of male coloration in this species has never been examined. Here, we present data on male
color (measured objectively using digital photography), dominance rank, measures of male-female association, and key sexual
behaviors, of adult male drills (n = 17) living in four semi free-ranging enclosures at the Drill Rehabilitation and Breeding Center in Nigeria. We test the
hypothesis that male coloration is a badge of status, indicating dominance rank, and the hypothesis that male coloration attracts
females. We found that male coloration did indicate rank, and that high ranking, strongly colored males were more likely to
associate with adult females, and more specifically with fully tumescent females. These males also engaged in more sexual
activity. However, measures of male-female association and sexual behaviors were not related to male color once rank had been
taken into consideration; i.e., for males of a given rank, females did not prefer those that were more colorful. We discuss
the results in light of what is known about the wild drill social system, in which unfamiliar individuals may come together
in “supergroups,” and in which quality indicators of competitive ability may be particularly important. 相似文献
84.
An eight-nucleotide sequence in the potato virus X 3' untranslated region is required for both host protein binding and viral multiplication. 总被引:3,自引:1,他引:2 下载免费PDF全文
Gel retardation and UV-cross-linking techniques were used to demonstrate that two tobacco proteins, with approximate molecular masses of 28 and 32 kDa, bind to a site within the 3' region of potato virus X (PVX) genomic RNA. The protein binding is specific, in that a 50-fold excess of unlabeled probe prevents formation of the complexes but no reduction is observed with a 2,000-fold molar excess of yeast tRNA. Complex formation is inhibited by poly(U) but is relatively unaffected by poly(A), poly(G), or poly(C-I). PVX RNA-host protein complex formation occurs in vitro at salt concentrations up to 400 mM. Deletion mapping indicates that the proteins bind within the 3' untranslated region (UTR) of PVX genomic RNA and that an 8-nucleotide U-rich sequence (5'-UAUUUUCU) is required for the binding. Deletion of the 8-nucleotide U-rich region from the 3' UTR of a sensitive PVX reporter virus that carries the luciferase gene in place of the PVX coat protein gene results in a more than 70,000-fold reduction in luciferase expression in tobacco protoplasts. RNA probes carrying the sequence GCGC in place of the central four contiguous uridines of the 8-nucleotide U-rich motif fail to bind host protein at detectable levels, and the same mutation, when introduced into the PVX reporter virus, eliminates viral multiplication. Mutations of 1 or 2 nucleotides within the same four uridines reduced both binding of host proteins and replication of reporter virus. These results indicate that the 8-nucleotide U-rich motif within the PVX 3' UTR is important for some aspect of viral multiplication and suggest that host protein binding plays a role in the process. 相似文献
85.
Se-Chan Kim Alexander Ghanem Heidi Stapel Klaus Tiemann Pascal Knuefermann Andreas Hoeft Rainer Meyer Christian Grohé Anne A Knowlton Georg Baumgarten 《BMC physiology》2007,7(1):5
Backgound
It has been reported that Toll-like receptor 4 (TLR4) deficiency reduces infarct size after myocardial ischemia/reperfusion (MI/R). However, measurement of MI/R injury was limited and did not include cardiac function. In a chronic closed-chest model we assessed whether cardiac function is preserved in TLR4-deficient mice (C3H/HeJ) following MI/R, and whether myocardial and systemic cytokine expression differed compared to wild type (WT). 相似文献86.
87.
Ye. P. Dulepova V. A. Nadtochii L. L. Budnikova 《Russian Journal of Marine Biology》2008,34(7):472-481
The trophic structure of benthic communities in the Sea of Okhotsk and Bering Sea was analyzed based on the results of macrobenthic
survey catches conducted in the Far Eastern seas in the 2000s. The production of trophic levels and entire communities was
determined. It was shown that production of benthic communities is still at a high level and has almost not changed since
the 1980s. The arrangement of the communities of some shelf areas by productivity characteristics was made. 相似文献
88.
Y. Jiang 《Biochimica et Biophysica Acta (BBA)/General Subjects》1994,1201(1):76-84
Proteolysis of the hydroxylase component of soluble methane monooxygenase (MMO) with trypsin yielded a protein which retained 50% activity in a standard MMO assay. In an H2O2-driven assay, in which H2O2 replaced two of the protein components, NADH and O2 used in the standard assay, the proteolysed hydroxylase retained full activity for ethane, propane and propene, but had a 2–3 fold increase with methane as substrate. Several crosslinking reagents have been tested for their ability to stabilise the proteolysed form of the hydroxylase. Using polyoxyethylene bis(imidazolyl carbonyl) (Mr 3350) as the crosslinking agent, increased thermostability of the hydroxylase was observed. Activated methoxypolyethylene glycol (Mr 5000) was used to modify the hydroxylase which was now soluble in organic solvents as well as water and could be activated by H2O2. The glycol-modified hydroxylase functioned well in organic solvents in the catalysis of propene oxidation. 相似文献
89.
Christelle Hély Kelly K. Caylor Peter Dowty Samuel Alleaume Robert J. Swap Hank H. Shugart Christopher O. Justice 《Ecosystems》2007,10(7):1116-1132
Abstract
We present a regional fuel load model (1 km2 spatial resolution) applied in the southern African savanna region. The model is based on a patch-scale production efficiency
model (PEM) scaled up to the regional level using empirical relationships between patch-scale behavior and multi-source remote
sensing data (spatio-temporal variability of vegetation and climatic variables). The model requires the spatial distribution
of woody vegetation cover, which is used to determine separate respiration rates for tree and grass. Net primary production,
grass and tree leaf death, and herbivory are also taken into account in this mechanistic modeling approach. The fuel load
model has been calibrated and validated from independent measurements taken from savanna vegetation in Africa southward from
the equator. A sensitivity analysis on the effect of climate variables (incoming radiation, air temperature, and precipitation)
has been conducted to demonstrate the strong role that water availability has in determining productivity and subsequent fuel
load over the southern African region. The model performance has been tested in four different areas representative of a regional
increasing rainfall gradient—Etosha National Park, Namibia, Mongu and Kasama, Zambia, as well as in Kruger National Park,
South Africa. Within each area, we analyze model output from three different magnitudes of canopy coverage (<5, 30, and 50%).
We find that fuel load ranges predicted by the model are globally in agreement with field measurements for the same year.
High rainfall sustains green herbaceous production late in the dry season and delays tree leaf litter production. Effect of
water on production varies across the rainfall gradient with delayed start of green material production in more arid regions. 相似文献
90.
M. V. Slavutskaya A. V. Kirenskaya V. Yu. Novototskii-Vlasov V. V. Shul'govskii I. B. Kozlovskaya 《Human physiology》2005,31(5):545-553
The parameters of saccades and presaccadic slow potentials were studied in right-handed men with a dominant right eye, including 19 schizophrenics and 12 healthy subjects. For visual stimulation, three light-emitting diodes were used, which were located in the center of the visual field (the central fixation stimulus) and 10° to the right and left of it (peripheral stimuli). Two stimulation protocols were used: with a simultaneous switching off of the central fixation stimulus and switching on of the peripheral stimuli (test 1) and with an interstimulus gap of 200 ms (test 2). According to the latency, saccades were divided into anticipatory, express, and regular. Slow EEG potentials preceding regular saccades were analyzed. It was found that the proportion of anticipatory saccades is considerably higher than the normal value in schizophrenia. The analysis of the presaccadic potentials demonstrated a significant decrease in the amplitude of negative potentials in the vertex region at early stages of presaccadic preparation and its increase in the occipital region at late stages. Test 2 in the patients demonstrated an increase in the positivity focus in the frontal region of the right hemisphere. It was assumed that the alterations found in schizophrenia result from the deficit of frontal cortical fields. 相似文献