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911.
Sung Tae Kim Kyung‐Mi Lee Hyun‐Joo Park Su‐Eon Jin Woong Shick Ahn Chong‐Kook Kim 《The journal of gene medicine》2009,11(1):26-37
Background
Interleukin (IL)‐13, overproduced in the skin of atopic dermatitis (AD), has been shown to play an essential role in the pathogenesis of the disease. Thus, inhibition of IL‐13 production should provide a key step to alleviate disease conditions of the atopic skin. In the present study, IL‐13 antisense oligonucleotide (ASO) was designed and formulated with cationic elastic liposome (cEL) to improve transdermal delivery.Methods
ASOs were generated against murine IL‐13 mRNA (+4 to + 23) and complexed with cEL. Physicochemical properties of IL‐13 ASO/cEL complex were examined by DNA retardation and DNase I protection assay. An in vitro inhibition study was performed in T‐helper 2 (Th2) cells and cytotoxicity was tested by the XTT assay. The in vivo effect of IL‐13 ASO/cEL complex was tested in a murine model of AD.Results
In vitro, the IL‐13 ASO/cEL complex showed dose‐ and ratio‐dependent inhibition of IL‐13 secretion in Th2 cells. At the IL‐13 ASO/cEL ratio of 6, maximum inhibition of IL‐13 secretion was observed. When applied to the ovalbumin‐sensitized murine model of AD, topically administered IL‐13 ASO/cEL complex dramatically suppressed IL‐13 production (by up to 70% of the control) in the affected skin region. In addition, the levels of IL‐4 and IL‐5 were also significantly reduced. Moreover, IL‐13 ASO/cEL‐treated AD mice showed reduced infiltration of inflammatory cells into the epidermal and dermal areas, with concomitant reduction of skin thickness.Conclusions
These data suggests that IL‐13 ASO/cEL complex can provide a potential therapeutic tool for the treatment of AD and also be applied to other immune diseases associated with the production of Il‐13. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献912.
Ming Shun Li Jong Yul Roh Xueying Tao Zi Niu Yu Zi Duo Liu Qin Liu Hong Guang Xu Hee Jin Shim Yang-Su Kim Yong Wang Jae Young Choi Yeon Ho Je 《Journal of microbiology (Seoul, Korea)》2009,47(4):466-472
Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki Kl which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pKlS-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pKlS-1, ORF2 (MobKl) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8~25.2%) with the Rep protein coded by RCR plasmids, however. The putative double-strand origin of replication (dso) and single-strand origin of replication (sso) of pKlS-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pKlS-1, seven subclones were contructed in the B. thuringiensis ori-negative pHTIK vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (ReplK), dso and ORF4, exhibited replication ability. These findings identified pKlS-1 as a new RCR group VII plasmid, and determined its replication region. 相似文献
913.
Je Hee Lee Seon Young Choi Yoon-Seong Jeon Hye Ri Lee Eun Jin Kim Binh Minh Nguyen Nguyen Tran Hien M. Ansaruzzaman M. Sirajul Islam Nurul A. Bhuiyan S. K. Niyogi B.L. Sarkar G. Balakrish Nair Dae Shick Kim Anna Lena Lopez Cecil Czerkinsky John D. Clemens Jongsik Chun Dong Wook Kim 《Journal of microbiology (Seoul, Korea)》2009,47(6):783-788
Analysis of the CTX prophage and RS1 element in hybrid and altered Vibrio cholera O1 strains showed two classifiable groups. Group I strains contain a tandem repeat of classical CTX prophage on the small chromosome. Strains in this group either contain no element(s) or an additional CTX prophage or RS1 element(s) on the large chromosome. Group II strains harbor RS1 and CTX prophage, which has an E1 Tor type rstR and classical ctxB on the large chromosome. 相似文献
914.
Hee Jin Shim Jae Young Choi Ming Shun Li Yong Wang Jong Yul Roh Soo-Dong Woo Byung Rae Jin Yeon Ho Je 《Journal of Asia》2009,12(4):217-220
A novel recombinant baculovirus, designated AcB5A, was constructed to develop an improved baculovirus insecticide with additional beneficial properties. Bacillus thuringiensis crystal protein gene (cry1–5) and an insect-specific neurotoxin gene (AaIT) were introduced into the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) genome by fusion of polyhedrin–cry1–5–polyhedrin under the control of polyhedrin gene promoter, and by insertion of AaIT under the control of early promoter of ORF3004 from Cotesia plutellae bracovirus. RT-PCR analysis with total RNA from AcB5A-infected cells indicated that cry1–5 and AaIT genes were normally transcribed. The 150 kDa of polyhedrin–Cry1–5–polyhedrin fusion protein was produced by AcB5A and occluded into polyhedra produced by the recombinant virus. This protein was activated when treated with trypsin to form a crystal protein of approximately 65 kDa. The AcB5A showed a high level of insecticidal activity against Plutella xylostella larvae and a significant reduction in the lethal time against Spodoptera exigua larvae compared to those infected with wild-type AcMNPV. The expression level of the fusion protein decreased after in vivo passage as a result of homologous recombination between the two polyhedrin genes. 相似文献
915.
Chang-Gi Kim Dae In Kim Hyo-Jeong Kim Ji Eun Park Bumkyu Lee Kee Woong Park Soon-Chun Jeong Kyung Hwa Choi Joo Hee An Kang-Hyun Cho Young Soon Kim Hwan Mook Kim 《Journal of Plant Biology》2009,52(3):251-258
We conducted a 2-year field assessment of the gene flow from genetically modified (GM) chili pepper (Capsicum annuum L.), containing the PepEST (pepper esterase) gene, to a non-GM control line “WT512” and two commercial hybrid cultivars, “Manidda” and “Cheongpung Myeongwol
(CM).” After seeds were collected from the pollen-recipient non-GM plants, hybrids between them and the GM peppers were screened
by a hygromycin assay. PCR with the targeting hpt gene was performed to confirm the presence of transgenes in hygromycin-resistant seedlings. Out of 7,071 “WT512” seeds and
6,854 “Manidda” seeds collected in 2006, eight and 12 hybrids, respectively, were detected. In 2007, 33 hybrids from 3,456
“WT512” seeds and 50 hybrids from 3,457 “CM” seeds were found. The highest frequency of gene flow, 6.19%, was observed in
that 2007 trial. These results suggest that a limited isolation distance would be sufficient to prevent gene flow from GM
to conventionally bred chili peppers. 相似文献
916.
Miscanthus sinensis is a promising species for biomass production. Influences of genetic and nongenetic factors on androgenesis induction efficiency
were investigated. This is the first report on successful induction of pollen-derived callus inM. sinensis. The callus yield was strongly affected by genotype. A beneficial influence of cold pretreatment of spikes on androgenesis
induction was observed. The highest yield of calli was obtained in cultures on a modified C17 medium. The results suggest
that the high callus yield might be caused by the late culture initiation. The beginning of anther culture at the end of the
flowering season caused a 17-fold increase in callus yield, in comparison to culture initiated at the height of the flowering
season (August). It is likely, however, that the efficiency of androgenesis induction in the case ofM. sinensis anther culture beginning in October could be related to a positive influence of growing donor plants in conditions of cooler
and shorter day, i.e. 11-h day with temperature around 11°C and 13-h night with temperature around 5°C. Results of this study
can significantly support the development of effective methods ofM. sinensis haploidization, which could be used in crop improvement by breeding. 相似文献
917.
In this study, we examined to what extent the internal site factors (light and soil conditions) are responsible for herb layer
diversity in oak-dominated forest stands growing on different substrates in central Bohemia (Czech Republic). We collected
data on herb layer diversity, light and nutrient availability at nine oak stands, representing the range of environmental
variability for these types of forests in the region. We found that species richness increased with light availability, but
only if the site occupied predominantly by fast-colonizing species was excluded from the analysis (P < 0.05). Species richness correlated positively with soil pH and negatively with nitrogen (N) concentration in humus (P < 0.05). The highest species richness was found at sites with not only low N soil concentration, but also simultaneously
with high phosphorus (P) soil concentration. Despite this finding, however, herb layer diversity is evidently threatened much
more in P-rich soils than in P-poor soils. It seems that the enhancement of N in an ecosystem due to litter accumulation and
N deposition generally leads to only a minor increase in N availability at P-poor sites, but a considerable increase at P-rich
sites. Therefore, species richness can be exceptionally high at P-rich sites, but only under conditions of strong N limitation. 相似文献
918.
Hyun Kyoung Lee Lih Wang Yoon Kyung Shin Duk Joon Suh 《Biochemical and biophysical research communications》2009,382(2):410-414
Interleukin-6 plays an important role in peripheral nerve regeneration. We recently reported that IL-6 targets Schwann cells in the peripheral nerve for its function. In this study, we analyzed genes whose expression is regulated by IL-6 in a cell line derived from Schwann cells, the peripheral glia, using the Illumina gene microarray. At measurements 3 and 12 h after IL-6 treatment, 35 genes were found to be upregulated by IL-6. Most upregulated genes were proinflammatory genes that are known to be induced in inflammatory conditions. Interestingly, the expression of immunoproteasome subunits was upregulated by IL-6 in Schwann cells. Treatment with forskolin, an agent that mimics axonal signaling, suppressed the expression of IL-6-inducible genes. Finally, we found for the first time that sciatic nerve injury induced immunoproteasome expression in vivo. These findings indicate that IL-6 is involved in peripheral nerve regeneration by regulating proinflammatory signaling in Schwann cells. 相似文献
919.
920.
Mina Song Jae Eun Park Sung Goo Park Hyung-Kyoon Choi Seong Eon Ryu Sayeon Cho 《Biochemical and biophysical research communications》2009,381(4):491-6837
Protein phosphorylation plays critical roles in many regulatory mechanisms controlling cell activities and thus involved in various diseases. The cellular equilibrium of phosphorylation is regulated through the actions of protein kinases and phosphatases. Therefore, these regulatory proteins have emerged as promising targets for drug development. In this study, we screened protein tyrosine phosphatases (PTPs) by in vitro phosphatase assays to identify PTPs that are inhibited by 8-hydroxy-7-(6-sulfonaphthalen-2-yl)diazenyl-quinoline-5-sulfonic acid (NSC-87877), a potent inhibitor of SHP-1 and SHP-2 PTPs. Phosphatase activity of dual-specificity protein phosphatase 26 (DUSP26) was decreased by the inhibitor in a dose-dependent manner. Kinetic studies with NSC-87877 and DUSP26 revealed a competitive inhibition. NSC-87877 effectively inhibited DUSP26-mediated dephosphorylation of p38, a member of mitogen-activated protein kinase (MAPK) family. Since DUSP26 is involved in survival of anaplastic thyroid cancer (ATC) cells, NSC-87877 could be a therapeutic reagent for treating ATC. 相似文献