全文获取类型
收费全文 | 3357篇 |
免费 | 405篇 |
出版年
2021年 | 43篇 |
2020年 | 30篇 |
2019年 | 31篇 |
2018年 | 43篇 |
2017年 | 48篇 |
2016年 | 50篇 |
2015年 | 123篇 |
2014年 | 120篇 |
2013年 | 123篇 |
2012年 | 142篇 |
2011年 | 148篇 |
2010年 | 102篇 |
2009年 | 96篇 |
2008年 | 113篇 |
2007年 | 123篇 |
2006年 | 125篇 |
2005年 | 132篇 |
2004年 | 104篇 |
2003年 | 132篇 |
2002年 | 97篇 |
2001年 | 84篇 |
2000年 | 77篇 |
1999年 | 69篇 |
1998年 | 40篇 |
1997年 | 41篇 |
1996年 | 34篇 |
1995年 | 39篇 |
1994年 | 44篇 |
1993年 | 33篇 |
1992年 | 65篇 |
1991年 | 74篇 |
1990年 | 62篇 |
1989年 | 83篇 |
1988年 | 61篇 |
1987年 | 67篇 |
1986年 | 50篇 |
1985年 | 45篇 |
1984年 | 63篇 |
1983年 | 40篇 |
1982年 | 36篇 |
1981年 | 30篇 |
1980年 | 39篇 |
1979年 | 51篇 |
1978年 | 49篇 |
1976年 | 39篇 |
1975年 | 34篇 |
1974年 | 43篇 |
1973年 | 42篇 |
1972年 | 33篇 |
1971年 | 31篇 |
排序方式: 共有3762条查询结果,搜索用时 15 毫秒
81.
82.
83.
W. Jahr K. Skiebe M. Stein 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1965,35(1-2):7-14
Ohne ZusammenfassungQuedlinburger Beiträge zur Züchtungsforschung Nr. 65Herrn Prof. Dr. Dr. h. c.Becker zum 60. Geburtstag. 相似文献
84.
85.
86.
D W Crabb P M Stein K M Dipple J B Hittle R Sidhu M Qulali K Zhang H J Edenberg 《Genomics》1989,5(4):906-914
87.
Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells 总被引:32,自引:0,他引:32
M A Aronow L C Gerstenfeld T A Owen M S Tassinari G S Stein J B Lian 《Journal of cellular physiology》1990,143(2):213-221
Rat calvaria osteoblasts derived from 21-day-old fetal rat pups undergo a temporal expression of markers of the osteoblast phenotype during a 5 week culture period. Alkaline phosphatase and osteocalcin are sequentially expressed in relation to collagen accumulation and mineralization. This pattern of expression of these osteoblast parameters in cultured rat osteoblasts (ROB) is analogous to that seen in vivo in developing fetal rat calvaria tissue (Yoon et. al: Biochem. Biophis. Res. Commun. 148:1129, 1987) and is similar to that observed in cultures of subcultivated 16-day-old embryonic chick calvaria-derived osteoblasts (COB) (Gerstenfeld, et.al: Dev. Biol. 122:46, 1987). While the cellular organization of subcultivated COB and primary ROB cultures are somewhat different, the temporal expression of the parameters remains. Both the rat and chick culture systems support formation of matrix mineralization even in the absence of beta-glycerol-phosphate. A systematic examination of factors which constitute conditions supporting complete expression of the osteoblast phenotype in ROB cultures indicate requirements for specific serum lots, ascorbic acid and the ordered deposition of mineral in the extracellular matrix. The present studies suggest that formation of a collagenous matrix, dependent on ascorbic acid, is requisite for expression of the osteoblast phenotype. In ROB cultures, expression of osteocalcin synthesis occurs subsequent to initiation of alkaline phosphatase activity and accompanies the formation of mineralized nodules. Thus, extracellular matrix mineralization (deposition of hydroxyapatite) is required for complete development of the osteoblast phenotype, as reflected by a 200-fold increase in osteocalcin synthesis. These data show the temporal expression of the various osteoblast parameters during the formation and mineralization of an extracellular matrix can provide markers reflective of various stages of osteoblast differentiation/maturation in vitro. 相似文献
88.
Analysis of E1A-mediated growth regulation functions: binding of the 300-kilodalton cellular product correlates with E1A enhancer repression function and DNA synthesis-inducing activity. 总被引:39,自引:16,他引:23
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Adenovirus E1A transforming function requires two distinct regions of the protein. Transforming activity is closely linked with the presence of a region designated conserved domain 2 and the ability of this region to bind the product of the cellular retinoblastoma tumor suppressor gene. We have investigated the biological properties of the second transforming region of E1A, which is located near the N terminus. Transformation-defective mutants containing deletions in the N terminus (deletion of residues between amino acids 2 and 36) were deficient in the ability to induce DNA synthesis and repress insulin enhancer-stimulated activity. The function of the N-terminal region correlated closely with binding of the 300-kilodalton E1A-associated protein and not with binding of the retinoblastoma protein. These results indicate that transformation by E1A is mediated by two functionally independent regions of the protein which interact with different specific cellular proteins and suggest that the 300-kilodalton E1A-associated protein plays a major role in E1A-mediated cell growth control mechanisms. 相似文献
89.
U Pauli J F Chiu P Ditullio P Kroeger V Shalhoub T Rowe G Stein J Stein 《Journal of cellular physiology》1989,139(2):320-328
Protein-DNA interactions within the promoter of a cell cycle-regulated human H4 histone gene were examined by binding of 5'-end-labeled DNA segments to Western blots of nuclear protein fractions. Specific protein interactions were observed with DNA segments located between -500 bp and -1,070 bp upstream of the ATG initiation codon and included a histone H1 binding segment flanked on both sides by binding sites for a 45 kD nuclear protein. This region of the gene contains a DNase I-sensitive site in the center (-720 to -820 bp), and sequence analysis revealed the presence of scaffold attachment sequences in the two flanking segments. Topoisomerase II consensus sequences and in vitro topoisomerase II cleavage sites were also detected in the two flanking segments. Our results suggest that the 45 kd nuclear protein may preferentially interact with these two segments of the H4 histone gene to mediate association with the nuclear matrix. The presence of negative regulatory elements in this putative matrix attachment region provides a basis for the speculation that such nuclear proteins are associated with alterations in gene-matrix interaction that are functionally related to gene expression. 相似文献
90.
Patricia A. Fraser Barbara Moore Rosanne Stein Sharon Alosco Armead H. Johnson Deborah Marcus-Bagley Zuhier Awdeh Edmond J. Yunis Chester A. Alper 《Immunogenetics》1990,31(2):89-93
We analyzed the frequency distribution of 106 complotypes [four allele sets of the major histocompatibility complex (MHC) genes for the complement proteins factor B, C2, C4A, and C4B] from 32 Black families residing in Boston and Washington, DC. Twenty-five different complotypes were identified, among which there were four complotypes that had not been previously observed in our large database of complotypes compiled from family studies of Boston Caucasians and that are, presumably, unique to individuals of African origin. These four African-derived complotypes areFC(1,90)0, FC63, S1C2,17, andSC(3,2,90)0. The frequencies of two of these four unique Black complotypes,FC(1,90)0 andFC63, were increased significantly when compared to Caucasians (pcorr <0.00042, pcorr=0.00294, respectively). The complotypeFC(1,90)0 was in positive linkage disequilibrium withHLA-DR3 haplotypes containing theB locus antigens Bw42, Bw52, Bw53, and Bw58, whileFC63 was associated withHLA-Bw70,-DR5. These findings demonstrate the extensive polymorphism of complotypes in Blacks, and also suggest that it may be possible to define unique extended haplotypes of African origin. 相似文献