全文获取类型
收费全文 | 694篇 |
免费 | 32篇 |
出版年
2022年 | 12篇 |
2021年 | 30篇 |
2020年 | 21篇 |
2019年 | 5篇 |
2018年 | 9篇 |
2017年 | 4篇 |
2016年 | 9篇 |
2015年 | 14篇 |
2014年 | 17篇 |
2013年 | 37篇 |
2012年 | 50篇 |
2011年 | 38篇 |
2010年 | 24篇 |
2009年 | 22篇 |
2008年 | 41篇 |
2007年 | 35篇 |
2006年 | 24篇 |
2005年 | 31篇 |
2004年 | 26篇 |
2003年 | 20篇 |
2002年 | 11篇 |
2001年 | 16篇 |
2000年 | 17篇 |
1999年 | 19篇 |
1998年 | 4篇 |
1997年 | 16篇 |
1996年 | 4篇 |
1995年 | 6篇 |
1994年 | 6篇 |
1992年 | 10篇 |
1991年 | 5篇 |
1990年 | 7篇 |
1989年 | 7篇 |
1988年 | 6篇 |
1987年 | 10篇 |
1986年 | 10篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 7篇 |
1982年 | 8篇 |
1981年 | 5篇 |
1980年 | 8篇 |
1979年 | 11篇 |
1978年 | 10篇 |
1976年 | 4篇 |
1973年 | 3篇 |
1972年 | 7篇 |
1966年 | 5篇 |
1965年 | 4篇 |
1963年 | 3篇 |
排序方式: 共有726条查询结果,搜索用时 46 毫秒
121.
R. Sachidanandham I. A. Ranjit Kumar M. R. V. Krishnan K. Jayaraman 《Bioprocess and biosystems engineering》1999,21(4):319-322
Alkaline protease is a class of important hydrolytic enzymes having wide applications in bioprocess industries. Their optimum pH in the alkaline range and stability at higher temperatures make them ideal in detergent and leather processing industries. These enzymes have excellent depilating capacity. The present study aims at process optimization for the production of alkaline protease from Bacillus amyloliquefaciens ATCC 23844. Information on the optimal operating temperature and pH were elicited from specific growth rates and alkaline protease yields. It was also observed that besides pH and temperature, the oxygen transfer rate is another important limiting variable for the production of protease. Volumetric oxygen transfer coefficient (k L a) was estimated at various impeller speeds and aeration rates. The optimal impeller speed and aeration rates were determined from k L a and the relative protease yield data. It was understood that the oxygen transfer rate is one of the crucial parameters for the production of proteolytic enzymes by B. amyloliquefaciens. 相似文献
122.
G Jayaraman T Krishnaswamy S Kumar C Yu 《The Journal of biological chemistry》1999,274(25):17869-17875
Snake venom cardiotoxins have been recently shown to block the enzymatic activity of phospholipid protein kinase and Na+,K+-ATPase. To understand the molecular basis for the inhibitory effects of cardiotoxin on the action of these enzymes, the nucleotide triphosphate binding ability of cardiotoxin analogue II (CTX II) from the Taiwan cobra (Naja naja atra) venom is investigated using a variety of spectroscopic techniques such as fluorescence, circular dichroism, and two-dimensional NMR. CTX II is found to bind to all the four nucleotide triphosphates (ATP, UTP, GTP, and CTP) with similar affinity. Detailed studies of the binding of dATP to CTX II indicated that the toxin molecule is significantly stabilized in the presence of the nucleotide. Molecular modeling, based on the NOEs observed for the dATP.CTX II complex, reveals that dATP binds to the CTX II molecule at the groove enclosed between the N- and C-terminal ends of the toxin molecule. Based on the results obtained in the present study, a molecular mechanism to account for the inhibition of the enzymatic activity of the phospholipid-sensitive protein kinase and Na+,K+-ATPase is also proposed. 相似文献
123.
124.
125.
126.
Jayaraman Narenkumar Sandhanasamy Devanesan Mohamad S. AlSalhi Seenivasan Kokilaramani Yen-Peng Ting Pattanathu K.S.M. Rahman Aruliah Rajasekar 《Saudi Journal of Biological Sciences》2021,28(12):7588
The present study has successfully identified the nitrate reducing bacteria present in the cooling water system and also investigated the performance of industrially applied biocide and inhibitor on the bacterial inhibition. In order to carry out the objective of this study, facilities and methods such as 16S rRNA gene sequencing, Lowry assay, SEM, EIS, ICP-MS and weight loss analysis were being utilized. In this study, two out of the five morphologically dis- similar colonies identified through 16S rRNA gene sequencing, namely the Massilia timonae and the Pseudomonas, were being utilized in the biocorrosion study on copper metal. From the surface analysis using SEM demonstrated the phenomenon of biofilm formation on the copper surface. 2-methylbenzimidazole has the addition of methyl group in the diazole ring position of benzimidazole it has create basicity environment and inhibit the metal deterioration. Meanwhile, it is also deducible from the EIS and protein analysis that com- bination of biocide with either of the inhibitors gives rise to better biocorrosion suppression (0.00178 mpy and 0.00171mpy) as compared to the sole effect of either biocide or inhibitor (0.00219 mpy, 0.00162 and 0.00143). Biocorrosion system biocide with MBM was found to exhibit 65% corrosion inhibition efficiency. Moreover, adoption of 2-Methylbenzimidazole seems to display better performance as compared to Multionic 8151, which is adopted in cooling water system. 相似文献
127.
Identification of the phosphorylation targets of symbiotic receptor‐like kinases using a high‐throughput multiplexed assay for kinase specificity 下载免费PDF全文
Dhileepkumar Jayaraman Alicia L. Richards Michael S. Westphall Joshua J. Coon Jean‐Michel Ané 《The Plant journal : for cell and molecular biology》2017,90(6):1196-1207
Detecting the phosphorylation substrates of multiple kinases in a single experiment is a challenge, and new techniques are being developed to overcome this challenge. Here, we used a multiplexed assay for kinase specificity (MAKS) to identify the substrates directly and to map the phosphorylation site(s) of plant symbiotic receptor‐like kinases. The symbiotic receptor‐like kinases nodulation receptor‐like kinase (NORK) and lysin motif domain‐containing receptor‐like kinase 3 (LYK3) are indispensable for the establishment of root nodule symbiosis. Although some interacting proteins have been identified for these symbiotic receptor‐like kinases, very little is known about their phosphorylation substrates. Using this high‐throughput approach, we identified several other potential phosphorylation targets for both these symbiotic receptor‐like kinases. In particular, we also discovered the phosphorylation of LYK3 by NORK itself, which was also confirmed by pairwise kinase assays. Motif analysis of potential targets for these kinases revealed that the acidic motif xxxsDxxx was common to both of them. In summary, this high‐throughput technique catalogs the potential phosphorylation substrates of multiple kinases in a single efficient experiment, the biological characterization of which should provide a better understanding of phosphorylation signaling cascade in symbiosis. 相似文献
128.
Purandare AV Chen Z Huynh T Pang S Geng J Vaccaro W Poss MA Oconnell J Nowak K Jayaraman L 《Bioorganic & medicinal chemistry letters》2008,18(15):4438-4441
This study reports the identification and Hits to Leads optimization of inhibitors of coactivator associated arginine methyltransferase (CARM1). Compound 7b is a potent, selective inhibitor of CARM1. 相似文献
129.
Distribution of thymine in protein coding mRNA sequences has been studied here. Our study suggest that thymine in protein coding sequences are not randomly distributed but with probability. Frame1 prefers to have definite amount of thymine. It is observed that the thymine content of frame 4 is also involved in protein coding. Frame 3 prefers to have least amount of thymine. However, frame 2 and frame 6 shows a variable degree of thymine content. The mRNA sequences of heterosexual animals, particularly, the human show a different distribution profile (less thymine in frame 1) compared to that of yeast and plants. 相似文献
130.
Manjunath Hegde Thomas K. Wood Arul Jayaraman 《Applied microbiology and biotechnology》2009,84(4):763-776