全文获取类型
收费全文 | 6303篇 |
免费 | 442篇 |
出版年
2024年 | 8篇 |
2023年 | 35篇 |
2022年 | 64篇 |
2021年 | 199篇 |
2020年 | 112篇 |
2019年 | 150篇 |
2018年 | 177篇 |
2017年 | 141篇 |
2016年 | 253篇 |
2015年 | 367篇 |
2014年 | 392篇 |
2013年 | 483篇 |
2012年 | 553篇 |
2011年 | 503篇 |
2010年 | 336篇 |
2009年 | 317篇 |
2008年 | 379篇 |
2007年 | 394篇 |
2006年 | 296篇 |
2005年 | 264篇 |
2004年 | 278篇 |
2003年 | 236篇 |
2002年 | 240篇 |
2001年 | 73篇 |
2000年 | 39篇 |
1999年 | 55篇 |
1998年 | 70篇 |
1997年 | 55篇 |
1996年 | 27篇 |
1995年 | 29篇 |
1994年 | 36篇 |
1993年 | 20篇 |
1992年 | 25篇 |
1991年 | 19篇 |
1990年 | 15篇 |
1989年 | 10篇 |
1988年 | 12篇 |
1987年 | 7篇 |
1986年 | 7篇 |
1985年 | 12篇 |
1984年 | 10篇 |
1983年 | 9篇 |
1982年 | 11篇 |
1981年 | 3篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1974年 | 3篇 |
1973年 | 4篇 |
排序方式: 共有6745条查询结果,搜索用时 31 毫秒
991.
PsbO, PsbP and PsbQ are the extrinsic proteins associated with the oxygen-evolving (OE) engine of all known higher plants. However their presence is not constant throughout all known oxy-photosynthetic organisms. For this reason, comparative analyses of the sequence and the structure of these proteins in different species from prokaryotes to eukaryotes may allow unravelling of the evolutionary track that they have followed and infer new hints about their function in the OE complex. The results show that PsbP and PsbQ present different evolutionary profiles, and that PsbQ is more closely associated to PsbO and probably to the manganese stabilizing role assigned to this protein. 相似文献
992.
The role of melanin- and carotenoid-based plumage coloration in nest defence in the Great Tit 总被引:1,自引:0,他引:1
Javier Quesada & Juan Carlos Senar 《Ethology : formerly Zeitschrift fur Tierpsychologie》2007,113(7):640-647
Although plumage coloration is recognized to convey valuable information about the bearer's parental abilities, few studies have explored the relationship between coloration and nest defence. In this study in Great Tit Parus major, we analysed the relationship between nest defence and melanin‐ as well as carotenoid‐based plumage coloration, after controlling for ecological variables known to influence nest defence. A principal components analysis was applied to classify birds according to how vigorously they defended the nest, and the intensity of nest defence was tested against plumage coloration. Males with a large black tie defended their nests more vigorously, but no such effect was found for yellow breast coloration. This suggests that melanin‐based coloration in the Great Tit is associated with aggression, including both dominance‐aggression and nest defence, whereas carotenoid‐based coloration is not. The challenge in future studies will be to demonstrate whether females use this trait as an ornament to assess male quality and whether they trade off between the different ornaments a male may exhibit. 相似文献
993.
Mariusz Lubomirski Michael R D'Andrea Stanley M Belkowski Javier Cabrera James M Dixon Dhammika Amaratunga 《Journal of computational biology》2007,14(3):350-359
Motivation: DNA microarrays are a well-known and established technology in biological and pharmaceutical research providing a wealth of information essential for understanding biological processes and aiding drug development. Protein microarrays are quickly emerging as a follow-up technology, which will also begin to experience rapid growth as the challenges in protein to spot methodologies are overcome. Like DNA microarrays, their protein counterparts produce large amounts of data that must be suitably analyzed in order to yield meaningful information that should eventually lead to novel drug targets and biomarkers. Although the statistical management of DNA microarray data has been well described, there is no available report that offers a successful consolidated approach to the analysis of high-throughput protein microarray data. We describe the novel application of a statistical methodology to analyze the data from an immune response profiling assay using human protein microarray with over 5000 proteins on each chip. 相似文献
994.
Measles Virus Fusion Protein Is Palmitoylated on Transmembrane-Intracytoplasmic Cysteine Residues Which Participate in Cell Fusion 总被引:4,自引:2,他引:2
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Monserrat Caballero Juan Carabaa Javier Ortego Rafael Fernndez-Muoz María L. Celma 《Journal of virology》1998,72(10):8198-8204
[3H]palmitic acid was metabolically incorporated into the viral fusion protein (F) of Edmonston or freshly isolated measles virus (MV) during infection of human lymphoid or Vero cells. The uncleaved precursor F0 and the F1 subunit from infected cells and extracellular virus were both labeled, indicating that palmitoylation can take place prior to F0 cleavage and that palmitoylated F protein was incorporated into virus particles. [3H]palmitic acid was released from F protein upon hydroxylamine or dithiothreitol treatment, indicating a thioester linkage. In cells transfected with the cloned MV F gene, in which the cysteines located in the intracytoplasmic and transmembrane domains (Cys 506, 518, 519, 520, and 524) were replaced by serine, a major reduction of [3H]palmitic acid incorporation was observed for F mutated at Cys 506 and, to a lesser extent, at Cys 518 and Cys 524. We also observed incorporation of [3H]palmitic acid in the F1 subunit of canine distemper virus F protein. Cell fusion induced by cotransfection of cells with MV F and H (hemagglutinin) genes was significantly reduced after replacement of Cys 506 or Cys 519 with serine in the MV F gene. Transfection with the F gene with a mutation for Cys 518 abolished cell fusion, although less mutant protein was detected on the cell surface. These results suggest that the F protein transmembrane domain cysteines 506 and 518 participate in structures involved in cell fusion, possibly mediated by palmitoylation. 相似文献
995.
Optimization of performance in collective systems often requires altruism. The emergence and stabilization of altruistic
behaviors are difficult to achieve because the agents incur a cost when behaving altruistically. In this paper, we propose
a biologically inspired strategy to learn stable altruistic behaviors in artificial multi-agent systems, namely reciprocal
altruism. This strategy in conjunction with learning capabilities make altruistic agents cooperate only between themselves,
thus preventing their exploitation by selfish agents, if future benefits are greater than the current cost of altruistic acts.
Our multi-agent system is made up of agents with a behavior-based architecture. Agents learn the most suitable cooperative
strategy for different environments by means of a reinforcement learning algorithm. Each agent receives a reinforcement signal
that only measures its individual performance. Simulation results show how the multi-agent system learns stable altruistic
behaviors, so achieving optimal (or near-to-optimal) performances in unknown and changing environments.
Received: 1 August 1997 / Accepted in revised form: 28 November 1997 相似文献
996.
Spores potentially dispersed to longer distances are more tolerant to ultraviolet radiation: A case study in the moss genus Orthotrichum
下载免费PDF全文
![点击此处可从《American journal of botany》网站下载免费的PDF全文](/ch/ext_images/free.gif)
997.
A coupled in vitro/in vivo approach for engineering a heterologous type III PKS to enhance polyketide biosynthesis in Saccharomyces cerevisiae
下载免费PDF全文
![点击此处可从《Biotechnology and bioengineering》网站下载免费的PDF全文](/ch/ext_images/free.gif)
998.
Christopher E. Doughty Gregory R. Goldsmith Nicolas Raab Cecile A. J. Girardin Filio Farfan‐Amezquita Walter Huaraca‐Huasco Javier E. Silva‐Espejo Alejandro Araujo‐Murakami Antonio C. L. da Costa Wanderley Rocha David Galbraith Patrick Meir Dan B. Metcalfe Yadvinder Malhi 《Biotropica》2018,50(1):16-25
Why do some forests produce biomass more efficiently than others? Variations in Carbon Use Efficiency (CUE: total Net Primary Production (NPP)/ Gross Primary Production (GPP)) may be due to changes in wood residence time (Biomass/NPPwood), temperature, or soil nutrient status. We tested these hypotheses in 14, one ha plots across Amazonian and Andean forests where we measured most key components of net primary production (NPP: wood, fine roots, and leaves) and autotrophic respiration (Ra; wood, rhizosphere, and leaf respiration). We found that lower fertility sites were less efficient at producing biomass and had higher rhizosphere respiration, indicating increased carbon allocation to belowground components. We then compared wood respiration to wood growth and rhizosphere respiration to fine root growth and found that forests with residence times <40 yrs had significantly lower maintenance respiration for both wood and fine roots than forests with residence times >40 yrs. A comparison of rhizosphere respiration to fine root growth showed that rhizosphere growth respiration was significantly greater at low fertility sites. Overall, we found that Amazonian forests produce biomass less efficiently in stands with residence times >40 yrs and in stands with lower fertility, but changes to long‐term mean annual temperatures do not impact CUE. 相似文献
999.
Zsófia Horváth Christophe Lejeusne Francisco Amat Javier Sánchez-Fontenla Csaba F. Vad Andy J. Green 《Hydrobiologia》2018,824(1):229-242
Long-term (1987–2015) monitoring data from Lake Lugano (Switzerland/Italy) were used to examine the effects of winter weather, solar radiation and mixing regime on the recovery of the trophic state of a lake undergoing nutrient management. Main hypotheses were that (H1) trends in trophic state were influenced not only by nutrient management, but also by winter weather and solar radiation, and (H2) the effects of management were more pronounced in the holomictic South basin than in the meromictic North basin of the lake. External loadings of phosphorus were strongly reduced during the study period, but key indicators of trophic state, including phosphorous concentration, primary production, chlorophyll a and deep-water oxygenation, showed inconsistent responses. Supporting H1, winter weather (parameterized using an index of the East Atlantic pattern) influenced temporal variation in phosphorus concentration and primary production in the North basin. Supporting H2, the effects of management on trophic state were clearer in the South basin, where most trophic-state indicators declined. Meromixis affected the restoration of the North basin lake by transmitting effects of climatic variation on trophic state. The added variability obscured the effects of restoration and caused sudden deteriorations in water quality, indicating that the restoration of meromictic lakes presents unique challenges. 相似文献
1000.
Rodrigo Megía-Palma Javier Martínez José J. Cuervo Josabel Belliure Octavio Jiménez-Robles Verónica Gomes Carlos Cabido Juli G. Pausas Patrick S. Fitze José Martín Santiago Merino 《International journal for parasitology》2018,48(9-10):709-718
Current and past parasite transmission may depend on the overlap of host distributions, potentially affecting parasite specificity and co-evolutionary processes. Nonetheless, parasite diversification may take place in sympatry when parasites are transmitted by vectors with low mobility. Here, we test the co-speciation hypothesis between lizard final hosts of the Family Lacertidae, and blood parasites of the genus Schellackia, which are potentially transmitted by haematophagous mites. The effects of current distributional overlap of host species on parasite specificity are also investigated. We sampled 27 localities on the Iberian Peninsula and three in northern Africa, and collected blood samples from 981 individual lizards of seven genera and 18 species. The overall prevalence of infection by parasites of the genus Schellackia was ~35%. We detected 16 Schellackia haplotypes of the 18S rRNA gene, revealing that the genus Schellackia is more diverse than previously thought. Phylogenetic analyses showed that Schellackia haplotypes grouped into two main monophyletic clades, the first including those detected in host species endemic to the Mediterranean region and the second those detected in host genera Acanthodactylus, Zootoca and Takydromus. All but one of the Schellackia haplotypes exhibited a high degree of host specificity at the generic level and 78.5% of them exclusively infected single host species. Some host species within the genera Podarcis (six species) and Iberolacerta (two species) were infected by three non-specific haplotypes of Schellackia, suggesting that host switching might have positively influenced past diversification of the genus. However, the results supported the idea that current host switching is rare because there existed a significant positive correlation between the number of exclusive parasite haplotypes and the number of host species with current sympatric distribution. This result, together with significant support for host–parasite molecular co-speciation, suggests that parasites of the genus Schellackia co-evolved with their lizard hosts. 相似文献