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排序方式: 共有550条查询结果,搜索用时 13 毫秒
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Evaluation of silibinin on the viability, migration and adhesion of the human prostate adenocarcinoma (PC-3) cell line 总被引:1,自引:0,他引:1
Prostate cancer (PCA) is the most common cancer diagnosed in men and the second most common cause of death due to cancers after lung cancer. Metastasis of cancer cells involves multiple processes and various cytophysiological changes, including changed adhesion capability between cells and extracellular matrix (ECM) and damaged intercellular interaction. Silibinin, a naturally occurring flavonoid antioxidant found in the milk thistle, has recently been shown to have potent antiproliferative effect against various malignant cell lines. In the present study, PC-3 cells were incubated with various concentrations of silibinin for different times; then, cell cytotoxicity, cell adhesion and cell motility were assessed using MTT assay, cell-matrix adhesion assay and cell migration assay, respectively. The results showed that silibinin exerted a dose- and time-dependent inhibitory effect on the viability, motility and adhesion of highly metastatic PC-3 cells. These observations indicate that silibinin can probably inhibit metastasis in PCA. 相似文献
95.
Hayden MR Karuparthi PR Habibi J Lastra G Patel K Wasekar C Manrique CM Ozerdem U Stas S Sowers JR 《Experimental biology and medicine (Maywood, N.J.)》2008,233(9):1109-1123
CONTEXT: The transgenic human islet amyloid polypeptide (HIP) rat model of type 2 diabetes mellitus (T2DM) parallels the functional and structural changes in human islets with T2DM. OBJECTIVE: The transmission electron microscope (TEM) was utilized to observe the ultrastructural changes in islet microcirculation. METHODS: Pancreatic tissue from male Sprague Dawley rats (2, 4, 8, 14 months) were used as controls (SDC) and compared to the 2-, 4-, 8- and 14-month-old HIP rat models. RESULTS: The 2-month-old HIP model demonstrated no islet or microcirculation remodeling changes when compared to the SDC models. The 4-month-old HIP model demonstrated significant pericapillary amyloid deposition and diminution of pericyte foot processes as compared to the SDC models. The 8-month-old model demonstrated extensive islet amyloid deposition associated with pericyte and beta-cell apoptosis when compared with SDC. The 14-month-old HIP model demonstrated a marked reduction of beta-cells and intra-islet capillaries with near complete replacement of islets by amyloidoses. Increased cellularity in the region of the islet exocrine interface was noted in the 4- to 14-month-old HIP models as compared to SDC. In contrast to intra-islet capillary rarefaction there was noticeable angiogenesis in the islet exocrine interface. Pericytes seemed to be closely associated with collagenosis, intra-islet adipogenesis and angiogenesis in the islet exocrine interface. CONCLUSION: The above novel findings regarding the microcirculation and pericytes could assist researchers and clinicians in a better morphological understanding of T2DM and lead to new strategies for prevention and treatment of T2DM. 相似文献
96.
Alamtaj Samsami Dehaghani Khatere Shahriary Mohammad Amin Kashef Sirous Naeimi Mohammad Javad Fattahi Zahra Mojtahedi Abbas Ghaderi 《Molecular biology reports》2009,36(8):2393-2397
IL-18, initially defined as a potent inducer of IFN- γ production, is a systemic, multifunctional cytokine with both pro-cancerous
and anti-cancer activities. The contribution of the IL-18 promoter polymorphisms at positions −607 (C/A) and −137 (G/C) to
cancer development has been reported. We sought to examine IL-18 serum level and its polymorphisms in Iranian women with ovarian
cancer. Single nucleotide polymorphisms (SNPs) at positions −607 (C/A) and −137 (G/C) were analyzed by allele-specific polymerase
chain reaction in 85 women with ovarian cancer and 158 healthy controls. IL-18 serum level was determined using ELISA method.
No significant association was found between the allele, genotype, and haplotype distributions of the SNPs and ovarian cancer.
Mean IL-18 serum level was significantly higher in patients than in controls (P = 0.008). Comparing IL-18 serum levels with genotypes at positions −607 and −137 revealed no significant difference. No association
was also found between IL-18 levels and the disease stage. In conclusion, our results indicate that IL-18 promoter polymorphisms
at positions −607 (C/A) and −137 (G/C) appear not to confer susceptibility to ovarian cancer in Iranian population; however,
IL-18 serum level increases in ovarian cancer patients. 相似文献
97.
Soltani F Mosaffa F Iranshahi M Karimi G Malekaneh M Haghighi F Behravan J 《Cell biology and toxicology》2009,25(3):291-296
The protective properties of a prenylated coumarin, umbelliprenin (UMB), on the human lymphocytes DNA lesions were tested.
Lymphocytes were isolated from blood samples taken from healthy volunteers. DNA breaks and resistance to H2O2-induced damage were measured using a single-cell microgel electrophoresis technique under alkaline conditions (comet assay).
Human lymphocytes were incubated in UMB (10, 25, 50, 100, 200, and 400 μM) alone or a combination of different concentrations
of UMB (10, 25, 50, 100, 200, and 400 μM) and 25 μM H2O2. Untreated cells, ascorbic acid (AA; 25, 50, 100, 200, and 400 μM) and H2O2 (25 μM) were considered as negative control, positive control, and the standard antioxidant agent for our study, respectively.
Single cells were analyzed with “TriTek Cometscore version 1.5” software. The DNA damage was expressed as percent tail DNA.
UMB exhibited a concentration-dependent increase in protection activity against DNA damage induced by 25 μM H2O2 (from 67.28% to 39.17%). The antigenotoxic activity of AA, in the range 0–50 μM, was greater than that of UMB. However, no
significant difference (p > 0.05) in the protective activity was found between UMB and AA at concentrations of approximately higher than 50 μM. 相似文献
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Mohammad Reza Zafarghandi Hassan Ravari Nasser Aghdami Mehrnaz Namiri Kasra Moazzami Ehsan Taghiabadi Abdorreza Fazel Behshad Pournasr Ali Farrokhi Ramezan Ali Sharifian Javad Salimi Majid Moini Hossein Baharvand 《Cytotherapy》2010,12(6):783-791
Background aimsThe aim was to investigate the therapeutic effect of granulocyte–colony-stimulating factor (G-CSF) administration following implantation of autologous bone marrow mononuclear cells (BM MNC) for patients with lower limb ischemia.MethodsThe design was a randomized controlled trial. Fifteen patients with severe chronic limb ischemia were treated with autologous BM MNC [without G-CSF (MNC–G-CSF) or combined with G-CSF administration for 5 days following transplantation (MNC+G-CSF)].ResultsAll clinical parameters, including ankle brachial index, visual analog scale and pain-free walking distance, showed a mean improvement from baseline, which was measured at 4 and 24 weeks after transplantation in both groups. However, in three (20%) patients, the clinical course did not improve and limb salvage was not achieved. No significant difference was observed among the patients treated in the MNC–G-CSF and MNC+G-CSF groups. No severe adverse reactions were reported during the study period. No relationship was observed between both the numbers of viable MNC or CD34+ cells and the clinical outcome.ConclusionsAutologous transplantation of BM MNC into ischemic lower limbs is safe, feasible and efficient for patients with severe peripheral artery disease. However, the administration of G-CSF following cell transplantation does not improve the effect of BM MNC implantation and therefore would not have any beneficial value in clinical applications of such cases. 相似文献