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911.
Robinson PW Costa DP Crocker DE Gallo-Reynoso JP Champagne CD Fowler MA Goetsch C Goetz KT Hassrick JL Hückstädt LA Kuhn CE Maresh JL Maxwell SM McDonald BI Peterson SH Simmons SE Teutschel NM Villegas-Amtmann S Yoda K 《PloS one》2012,7(5):e36728
The mesopelagic zone of the northeast Pacific Ocean is an important foraging habitat for many predators, yet few studies have addressed the factors driving basin-scale predator distributions or inter-annual variability in foraging and breeding success. Understanding these processes is critical to reveal how conditions at sea cascade to population-level effects. To begin addressing these challenging questions, we collected diving, tracking, foraging success, and natality data for 297 adult female northern elephant seal migrations from 2004 to 2010. During the longer post-molting migration, individual energy gain rates were significant predictors of pregnancy. At sea, seals focused their foraging effort along a narrow band corresponding to the boundary between the sub-arctic and sub-tropical gyres. In contrast to shallow-diving predators, elephant seals target the gyre-gyre boundary throughout the year rather than follow the southward winter migration of surface features, such as the Transition Zone Chlorophyll Front. We also assessed the impact of added transit costs by studying seals at a colony near the southern extent of the species' range, 1,150 km to the south. A much larger proportion of seals foraged locally, implying plasticity in foraging strategies and possibly prey type. While these findings are derived from a single species, the results may provide insight to the foraging patterns of many other meso-pelagic predators in the northeast Pacific Ocean. 相似文献
912.
913.
Lise Laurin Ben Amor Till M. Bachmann Jane Bare Christoph Koffler Serge Genest Philipp Preiss Jason Pierce Barclay Satterfield Bruce Vigon 《The International Journal of Life Cycle Assessment》2016,21(4):443-447
When life cycle assessment (LCA) results do not show a clear and certain environmental preference of one choice over one or several alternatives, current methods are limited in their ability to inform decision-makers. To address this and related cross-cutting issues, a group of LCA practitioners has been working on a roadmap for capacity development in LCA. The roadmap is identifying common needs for development in LCA, which can then be addressed by the broader LCA community. The roadmap document on decision-making support, having undergone a public comment period, outlines the current state as well as needs and milestones to ensure progress continues apace. The roadmap document, available for download, covers five main areas of development: (1) performance measures of confidence, which identify the acceptable uncertainty for study results, while minimizing expenditures; (2) selection of impact categories, an area with multiple existing methods. The roadmap suggests codifying these methods and identifying their suitability to various applications; (3) normalization; while several methods of normalization are in use, the method with the greatest acceptance in the LCA community (i.e., relying on total or per capita regional emissions/extractions) has a number of methodological drawbacks; (4) weighting, which is a form of multi-criteria decision analysis (MCDA). The broader MCDA field can enrich LCA by providing studied methods of assessing trade-offs; and (5) visualization of results. Many other LCA capacity needs would benefit from documentation. These include but are not limited to the following: addressing ill-characterized uncertainty, life cycle inventory data needs, data format needs, and tool capabilities. Other roadmapping groups are forming and are looking for practitioners to support the effort. 相似文献
914.
Genome-wide profiling and analysis of Arabidopsis siRNAs 总被引:3,自引:0,他引:3
915.
Hoban SM Borkowski DS Brosi SL McCleary TS Thompson LM McLachlan JS Pereira MA Schlarbaum SE Romero-Severson J 《Molecular ecology》2010,19(22):4876-4891
The spatial distribution of genetic diversity is a product of recent and historical ecological processes, as well as anthropogenic activities. A current challenge in population and conservation genetics is to disentangle the relative effects of these processes, as a first step in predicting population response to future environmental change. In this investigation, we compare the influence of contemporary population decline, contemporary ecological marginality and postglacial range shifts. Using classical model comparison procedures and Bayesian methods, we have identified postglacial range shift as the clear determinant of genetic diversity, differentiation and bottlenecks in 29 populations of butternut, Juglans cinerea L., a North American outcrossing forest tree. Although butternut has experienced dramatic 20th century decline because of an introduced fungal pathogen, our analysis indicates that recent population decline has had less genetic impact than postglacial recolonization history. Location within the range edge vs. the range core also failed to account for the observed patterns of diversity and differentiation. Our results suggest that the genetic impact of large-scale recent population losses in forest trees should be considered in the light of Pleistocene-era large-scale range shifts that may have had long-term genetic consequences. The data also suggest that the population dynamics and life history of wind-pollinated forest trees may provide a buffer against steep population declines of short duration, a result having important implications for habitat management efforts, ex situ conservation sampling and population viability analysis. 相似文献
916.
Noncoding Flavivirus RNA Displays RNA Interference Suppressor Activity in Insect and Mammalian Cells
Esther Schnettler Mark G. Sterken Jason Y. Leung Stefan W. Metz Corinne Geertsema Rob W. Goldbach Just M. Vlak Alain Kohl Alexander A. Khromykh Gorben P. Pijlman 《Journal of virology》2012,86(24):13486-13500
West Nile virus (WNV) and dengue virus (DENV) are highly pathogenic, mosquito-borne flaviviruses (family Flaviviridae) that cause severe disease and death in humans. WNV and DENV actively replicate in mosquitoes and human hosts and thus encounter different host immune responses. RNA interference (RNAi) is the predominant antiviral response against invading RNA viruses in insects and plants. As a countermeasure, plant and insect RNA viruses encode RNA silencing suppressor (RSS) proteins to block the generation/activity of small interfering RNA (siRNA). Enhanced flavivirus replication in mosquitoes depleted for RNAi factors suggests an important biological role for RNAi in restricting virus replication, but it has remained unclear whether or not flaviviruses counteract RNAi via expression of an RSS. First, we established that flaviviral RNA replication suppressed siRNA-induced gene silencing in WNV and DENV replicon-expressing cells. Next, we showed that none of the WNV encoded proteins displayed RSS activity in mammalian and insect cells and in plants by using robust RNAi suppressor assays. In contrast, we found that the 3′-untranslated region-derived RNA molecule known as subgenomic flavivirus RNA (sfRNA) efficiently suppressed siRNA- and miRNA-induced RNAi pathways in both mammalian and insect cells. We also showed that WNV sfRNA inhibits in vitro cleavage of double-stranded RNA by Dicer. The results of the present study suggest a novel role for sfRNA, i.e., as a nucleic acid-based regulator of RNAi pathways, a strategy that may be conserved among flaviviruses. 相似文献
917.
The TRPV ion channels mediate responses to many sensory stimuli including heat, low pH, neuropeptides, and chemical ligands. All TRPV subfamily members contain an intracellular N-terminal ankyrin repeat domain (ARD), a prevalent protein interaction motif. The 1.6-A crystal structure of the TRPV2-ARD, with six ankyrin repeats, reveals several atypical structural features. Repeats one through three display unusually long and flexible fingers with a large number of exposed aromatic residues, whereas repeats five and six have unusually long outer helices. Furthermore, a large counterclockwise twist observed in the stacking of repeats four and five breaks the regularity of the domain, altering the shape of surfaces available for interactions with proteins or other cellular ligands. Both solution studies and crystal packing interactions indicate that the TRPV2-ARD does not form homo-oligomers, suggesting that the ARD of TRPV ion channels may be used for interactions with regulatory factors rather than in promoting tetrameric assembly of the ion channels. 相似文献
918.
Iain Robinson Michael Andreas Ochsenkühn Colin J. Campbell Gerard Giraud William J. Hossack Jochen Arlt Jason Crain 《Journal of biophotonics》2010,3(3):138-146
Intracellular imaging is a key tool in the investigation of host‐pathogen interactions. Advances in this area are particularly sought to understand the effect of viral infection processes on the host cell and its metabolic functions including those cases where host cell lipid metabolism is modulated as a result of infection. We demonstrate the use of combined coherent anti‐Stokes Raman scattering (CARS) and two‐photon fluorescence microscopies to image fibroblast cells infected by cytomegalovirus. CARS is used to image the host cell membrane, lipid droplets and morphology of the nucleus. Cell nuclei are found to expand during infection, approximately doubling in area. Some cells also show accumulations of lipid droplets at the nuclear periphery. Using a genetically modified virus strain expressing the green fluorescent protein also enables two‐photon imaging of the same cells to reveal the location, nature and extent of viral protein expression. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
919.
Correigh M. Greene Jason E. Hall Kimberly R. Guilbault Thomas P. Quinn 《Biology letters》2010,6(3):382-386
A principle shared by both economists and ecologists is that a diversified portfolio spreads risk, but this idea has little empirical support in the field of population biology. We found that population growth rates (recruits per spawner) and life-history diversity as measured by variation in freshwater and ocean residency were negatively correlated across short time periods (one to two generations), but positively correlated at longer time periods, in nine Bristol Bay sockeye salmon populations. Further, the relationship between variation in growth rate and life-history diversity was consistently negative. These findings strongly suggest that life-history diversity can both increase production and buffer population fluctuations, particularly over long time periods. Our findings provide new insights into the importance of biocomplexity beyond spatio-temporal aspects of populations, and suggest that maintaining diverse life-history portfolios of populations may be crucial for their resilience to unfavourable conditions like habitat loss and climate change. 相似文献
920.
Christina L. Hutson Jason A. Abel Darin S. Carroll Victoria A. Olson Zachary H. Braden Christine M. Hughes Michael Dillon Consuelo Hopkins Kevin L. Karem Inger K. Damon Jorge E. Osorio 《PloS one》2010,5(1)
Although monkeypox virus (MPXV) studies in wild rodents and non-human primates have generated important knowledge regarding MPXV pathogenesis and inferences about disease transmission, it might be easier to dissect the importance of virulence factors and correlates of protection to MPXV in an inbred mouse model. Herein, we compared the two clades of MPXV via two routes of infection in the BALB/c and C57BL/6 inbred mice strains. Our studies show that similar to previous animal studies, the Congo Basin strain of MPXV was more virulent than West African MPXV in both mouse strains as evidenced by clinical signs. Although animals did not develop lesions as seen in human MPX infections, localized signs were apparent with the foot pad route of inoculation, primarily in the form of edema at the site of inoculation; while the Congo Basin intranasal route of infection led to generalized symptoms, primarily weight loss. We have determined that future studies with MPXV and laboratory mice would be very beneficial in understanding the pathogenesis of MPXV, in particular if used in in vivo imaging studies. Although this mouse model may not suffice as a model of human MPX disease, with an appropriate inbred mouse model, we can unravel many unknown aspects of MPX pathogenesis, including virulence factors, disease progression in rodent hosts, and viral shedding from infected animals. In addition, such a model can be utilized to test antivirals and the next generation of orthopoxvirus vaccines for their ability to alter the course of disease. 相似文献