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51.
Stefanie Henning Michael Mormann Jasna Peter-Katalinić Gottfried Pohlentz 《Amino acids》2011,41(2):343-350
α- and β-chains of hemoglobins derived from several species were analyzed directly from diluted blood samples by simultaneous
in-capillary proteolytic digestion and nanoESI MS and MS/MS analysis. Starting from fresh or frozen and thawed blood samples,
sequence coverages of >80% were usually obtained. Only 2 h after resuspension of a dried blood spot, human origin could be
demonstrated from data obtained by in-capillary tryptic digestion, nanoESI mass spectrometric analysis, and data base search.
A fast and facile differentiation of closely related species by hemoglobin-derived proteolytic “marker peptides” was demonstrated
for Asian (Elephas maximus) and African elephants (Loxodonta africana). Finally, amino acid sequences deduced from collision-induced dissociation experiments during in-capillary proteolytic digestion
of the corresponding blood samples allowed de novo sequencing of previously unknown sequences of hemoglobin chains of the
Patagonian cavy (Dolichotum patagona) and the Persian gazelle (Gazella subgutturosa subgutturosa). 100% of the α-chain sequences and more than 85% of the β-chain sequences were covered for both the species. Additionally,
sequence data derived from tandem MS experiments obtained with the Q-Tof analyzer were confirmed by high resolution Fourier-transform
ion cyclotron resonance mass spectrometric experiments. Accurate protein mass determination of the intact hemoglobin chains
directly from the corresponding blood samples by use of a Fourier-transform ion cyclotron resonance mass spectrometer corroborated
the deduced sequences of the respective α-chains. The present study demonstrates that in-capillary digestion allows fast characterization
and/or sequencing of hemoglobin chains directly from blood samples. 相似文献
52.
Sprouted and Freeze‐Dried Wheat and Oat Seeds – Phytochemical Profile and in Vitro Biological Activities 下载免费PDF全文
Naji Elhadi Aborus Vesna Tumbas Šaponjac Jasna Čanadanović‐Brunet Gordana Ćetković Alyssa Hidalgo Jelena Vulić Vanja Šeregelj 《化学与生物多样性》2018,15(8)
This research was carried out to study phytochemical profile, in vitro antioxidant capacity, reducing power, anti‐hyperglycemic, anti‐inflammatory activities and simulated gastrointestinal digestion of 7‐day old cereal sprouts: spelt wheat ‘Nirvana’ (WSSpe), wheat ‘Simonida’ (WSSim), oat ‘Golozrni’ (OSG) and oat ‘Jadar’ (OSJ). OSG expressed significantly higher (P ≤ 0.05) total phenols (TPC) and flavonoids content (TFC), antioxidant capacities (DPPH and ABTS assays) and reducing power (EC50DPPH = 2.12 mg/ml; EC50ABTS = 0.87 mg/ml; EC0.5RP = 12.24 mg/ml) as well as anti‐hyperglycemic activity (EC50AHgA = 0.96 mg/ml). WSSpe had the highest content of chlorophyll (131.23 mg/100 g) and carotenoids (22.84 mg/100 g). WSSim possessed the most potent anti‐inflammatory activity (2.71 mg/ml), though not significantly different from OSG (2.77 mg/ml). The in vitro simulation of gastro‐intestinal digestion showed higher release of phenolic compounds in intestinal than in gastric fluid. 相似文献
53.
Miloš Vittori Mohammed Khurshed Daisy I. Picavet Cornelis J.F. van Noorden Jasna Štrus 《Arthropod Structure & Development》2018,47(2):199-213
Calcium bodies are internal epithelial sacs found in terrestrial isopods of the family Trichoniscidae that contain a mineralized extracellular matrix that is deposited and resorbed in relation to the molt cycle. Calcium bodies in several trichoniscids are filled with bacteria, the function of which is currently unknown. The woodlouse Hyloniscus riparius differs from other trichoniscids in that it possesses two different pairs of calcium bodies, the posterior pair being filled with bacteria and the anterior pair being devoid of bacteria. We explored the development of these organs and bacterial colonization of their lumen during the postmarsupial development with the use of optical clearing and whole-body confocal imaging of larval and juvenile stages. Our results show that calcium bodies are formed as invaginations of the epidermis in the region of intersegmental membranes during the postmarsupial development. The anterior pair of calcium bodies is generated during the first postmarsupial manca stage, whereas the posterior calcium bodies first appear in juveniles and are immediately colonized by bacteria, likely through a connection between the calcium body lumen and the body surface. Mineral is deposited in calcium bodies as soon as they are present. 相似文献
54.
Jasna Bingulac-Popovic F. Figueroa Akie Sato William S. Talbot Stephen L. Johnson Michael Gates J. H. Postlethwait Jan Klein 《Immunogenetics》1997,46(2):129-134
The mammalian major histocompatibility complex (Mhc) consists of three closely linked regions, I, II, and III, occupying a single chromosomal segment. The class I loci in region
I and the class II loci in region II are related in their structure, function, and evolution. Region III, which is intercalated
between regions I and II, contains loci unrelated to the class I and II loci, and to one another. There are indications that
a similar Mhc organization exists in birds and amphibians. Here, we demonstrate that in the zebrafish (Danio rerio), a representative of the teleost fishes, the class II loci are divided between two linkage groups which are distinct from
the linkage group containing the class I loci. The β2-microglobulin-encoding gene is loosely linked to one of the class II loci. The gene coding for complement factor B, which
is one of the region III genes in mammals, is linked neither to the class I nor to the class II loci in the zebrafish. These
results, combined with preliminary data suggesting that the class I and class II regions in another order of teleost fish
are also in different linkage groups, indicate that close linkage of the two regions is not necessary either for regulation
of expression or for co-evolution of the class I and class II loci. They also raise the question of whether linkage of the
class I and class II loci in tetrapods is a primitive or derived character.
Received: 16 December 1996 / Revised: 6 February 1997 相似文献
55.
Johannes Müthing Jasna Peter-Katalinić Franz-Georg Hanisch Ulrich Neumann 《Glycoconjugate journal》1991,8(5):414-423
YAC-1 cells were propagated in bioreactors in 11 and 7.51 volumes. The cells were metabolically labelled withd-[1-14C]galactose andd-[1-14C]glucosamine. The ganglioside fraction, purified by DEAE-Sepharose and silica gel column chromatography, showed on thin layer chromatography four major bands with mobilities between GM1 and GD1a. Gangliosides, obtained by further purification steps including high performance liquid chromatography on silica gel 60 columns with a gradient system of isopropanol:hexane:water, and preparative high performance TLC were characterized by (1) immunostaining of corresponding asialogangliosides obtained by mild acid hydrolysis and neuraminidase treatment and (2) fast atom bombardment mass spectrometry of native and permethylated samples and methylation analysis of GM1b ganglioside. As well as small amounts of GM2 and GM1, the major gangliosides found in the complex mixture were GM1b and GalNAc-GM1b. The structural heterogeneity of these gangliosides was cased by (a) substitution of the ceramide moiety by fatty acids of different chain length and degree of unsaturation (C16:0, C24:0, C24:1) and (b) N-substitution of the sialic acid moieties with either acetyl or glycolyl groups. Disialogangliosides were detected only in low amounts and will be the subject of further investigation. A polyclonal chicken antiserum was raised against IVNeuAc-GgOse5Cer. The antiserum was highly specific for gangliosides (IVNeuAc and IVNeuGc) and asialogangliosides with a GgOse5Cer backbone. No cross-reaction with GM1b or GgOse4Cer was observed.
Abbreviations: FAB-MS, fast atom bombardment mass spectrometry; GSL(s), glycosphingolipid(s); HPLC, high performance liquid chromatography, HPTLC, high performance thin layer chromatography; NK, natural killer; SIM, selective ion monitoring; TIC, total ion current. NeuAc,N-acetylneuraminic acid; NeuGc,N-glycolylneuraminic acid. The designation of the following glycosphingolipids follows the IUB-IUPAC recommendations. GgOse3Cer or gangliotriaosylceramide or asialo GM2, GalNAc1-4Gal1-4GlcCer; GgOse4Cer or gangliotetraosylceramide or asialo GM1, Gal1-3GalNAc1-4Gal1-4GlcCer; GgOse5Cer organgliopentaosylceramide, GalNAc1-4Gal1-3GalNAc1-4Gal1-4GlcCer; II3NeuAc-GgOse4Cer or GM1; IV3NeuAcGgOse4Cer or GM1b; IV3NeuAc-GgOse5Cer or GalNAc-GM1b; IV3NeuAc, II3NeuAc-GgOse4Cer or GD1a; II3(NeuAc)2-GgOse4Cer or GD1b; IV3(NeuAc)2-GgOse4Cer or GD1c; IV3NeuAc,III6NeuAc-GgOse4Cer or GD1a; IV3NeuAc,II3(NeuAc)2-GgOse4Cer or GT1b;Vibrio cholerae and Arthrobacter ureafaciens neuraminidase (EC 3.2.1.18). 相似文献
56.
Jasna Arapov Sanda Skejić Mia Bužančić Ana Bakrač Olja Vidjak Natalia Bojanić Ivana Ujević Živana N. Gladan 《Phycological Research》2017,65(4):280-290
The aim of our research was to study the composition of Pseudo‐nitzschia species during a period when neurotoxin domoic acid (DA) was present in shellfish. Sampling was conducted in Ka?tela Bay (Central Adriatic Sea), between November 2015 and January 2016. Concentrations of DA analyzed in various shellfish species were low, below the regulatory limit, while the highest abundance of Pseudo‐nitzschia spp. reached 1.85 × 105 cells L?1 in the surface layer, at the beginning of November. Within the temperature and salinity range recorded during the investigated period, a positive correlation of Pseudo‐nitzschia spp. abundance was recorded with temperature. Morphological analyses by scanning electron microscopy revealed the presence of five Pseudo‐nitzschia species that had already been reported in the Adriatic Sea – P. calliantha, P. delicatissima, P. fraudulenta, P. pseudodelicatissima /P. cuspidata and P. subfraudulenta – as well as an unknown Pseudo‐nitzschia sp. The composition of the Pseudo‐nitzschia assemblage changed over the investigated period. The species P. pseudodelicatissima/P.cuspidata was found throughout the entire period and the highest diversity was noticed in January, when all six observed species were recorded. These results represent the first taxonomical investigation of the genus Pseudo‐nitzschia in Ka?tela Bay, as well as the first report of DA in shellfish from this area. 相似文献
57.
58.
Novak-Laus K Milicić J Tedeschi-Reiner E Iveković R Korsić J Zrinsćak O Mandić Z 《Collegium antropologicum》2006,30(1):163-170
The primary open-angle glaucomas are a group of diseases that have in common characteristic morphological changes at the optic nerve head and retinal nerve fiber layer, progressive retinal ganglion cells death and characteristic visual field loss. The risk for primary open angle glaucoma rises continuously with the level of the intraocular pressure. The disease advances slowly and there are no symptoms. Primary open angle glaucoma is caused by abnormal aqueous humour outflow in the trabecular meshwork in the open angle. Etiopathogenesis of primary open angle glaucoma is unclear. The increased risk of glaucoma in relatives has long been recognized. Frequency for manifestation of the disease is 10-30% in family members. The discovery of the specific gene loci responsible for the manifestation of glaucoma has helped us to understand its mechanism of origin and definitely confirmed the hereditary nature of this disease. Digito-palmar dermatoglyphs were already used to determine hereditary base of many diseases and it was the reason for investigation of their qualitative patterns in patients with glaucoma (22 males and 23 females), their immediate relatives (19 males and 23 females) in comparison to a group of phenotypically healthy population (52 males and 56 females). The results pointed a connection with the dermatoglyphic traits of the digito-palmar complex between patients with glaucoma and their immediate relatives. There is a possible discrimination of patients and their immediate relatives from phenotypically healthy population, too. 相似文献
59.
Šárka Perutková Mojca Frank Klemen Bohinc Goran Bobojevič Jasna Zelko Blaž Rozman Veronika Kralj-Iglič Aleš Iglič 《The Journal of membrane biology》2010,236(1):43-53
In biological systems, charged membrane surfaces are surrounded by charged molecules such as electrolyte ions and proteins.
Our recent experiments in the systems of giant phospholipid vesicles indicated that some of the blood plasma proteins (macro-ions)
may promote adhesion between equally charged membrane surfaces. In this work, theory was put forward to describe an IgG antibody-mediated
attractive interaction between negatively charged membrane surfaces which was observed in experiments on giant phospholipid
vesicles with cardiolipin-containing membranes. The attractive interactions between negatively charged membrane surfaces in
the presence of negatively and positively charged spherical macro-ions are explained using functional density theory and Monte
Carlo simulations. Both, the rigorous solution of the variational problem within the functional density theory and the Monte
Carlo simulations show that spatial and orientational ordering of macro-ions may give rise to an attractive interaction between
negatively charged membrane surfaces. It is also shown that the distinctive spatial distribution of the charge within the
macro-ions (proteins) is essential in this process. 相似文献