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51.
Pritam Mohanty Shashikanta Behera Swasti S. Swain Durga P. Barik Soumendra K. Naik 《Physiology and Molecular Biology of Plants》2013,19(4):605-610
An optimized protocol was developed for in vitro plant regeneration of a medicinally important herb Hedychium coronarium J. Koenig using sprouted buds of rhizomes. The rhizomes with sprouted bud were inoculated on Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium (MS) supplemented with either N6-benzyladenine (BA) alone (1.0–4.0 mg L−1) or in combination with 0.5 mg L−1 naphthalene acetic acid (NAA). Of these combinations, MS supplemented with a combination of 2.0 mg L−1 BA and 0.5 mg L−1 NAA was most effective. In this medium, best shoots (3.6) and roots (4.0) regeneration was observed simultaneously with an average shoot and root length of 4.7 cm and 4.2 cm respectively. Regeneration of shoots and roots in the same medium at the same time (One step shoot and root regeneration) reduced the time for production of in vitro plantlets and eliminates the media cost of rooting. Cent-percent (100 %) success in plant establishment was observed in both gradual acclimatization process as well as when plants were directly transferred to outdoor in clay pots containing a mixture of garden soil and sand (2:1) without any sequential acclimatization stage. 相似文献
52.
Gouda Gayatri Gupta Manoj Kumar Donde Ravindra Mohapatra Trilochan Vadde Ramakrishna Behera Lambodar 《Physiology and Molecular Biology of Plants》2020,26(5):885-898
Physiology and Molecular Biology of Plants - Continuous rise in the human population has resulted in an upsurge in food demand, which in turn demand grain yield enhancement of cereal crops,... 相似文献
53.
Sailabala?PadhiEmail author Prasanna?K.?Swain Sasmita?K.?Behura Sivaram?Baidya Santosh?K.?Behera Manas?R.?Panigrahy 《Journal of applied phycology》2011,23(2):151-155
The agarophyte red alga Gracilaria verrucosa occurs widely in Chilika Lake, one of the RAMSAR wetland sites in India. The lake is situated in the extreme southeast corner
of Orissa between latitudes 19°28′ and 19°54′ N and longitudes 85°06′ and 85°35′ E. The natural biomass production is not
sufficient for the agar industry, and the only alternative is to maximize the production of the seaweed through mass cultivation
by seaweed farming. To elucidate important aspects of the growth and development of G. verrucosa, experimental field cultivation was undertaken at Langaleswar and Samal sites of Chilika Lake using ropes and raft methods
during March to August, 2009. After 30 days of cultivation a maximum 15- and 13.8-fold increase in biomass in raft culture
and rope culture, respectively, was observed at Langaleswar and an 11.6- and 11.0-fold increase in biomass at Samal. Environmental
parameters such as temperature, salinity, pH, transparency, DO, conductivity, nitrate, and phosphate were monitored at both
stations, and the influence of environmental parameters is discussed. 相似文献
54.
S.K. Behera P.P. Panda S. Singh N. Pradhan L.B. Sukla B.K. Mishra 《International biodeterioration & biodegradation》2011,65(7):1035-1042
Depletion of high-grade ores and presence of significant quantities of metals in low-grade oxide ores has enforced to utilize the overburdens (COB) and wastes (low-grade ores) generated during mining operations. The impact of ore mineralogy and mineral–microbe interaction during bioleaching could not be ignored. Seeking to the need, a systematic study was performed to establish the reaction mechanism involved for recovery of nickel and cobalt from chromite overburden (COB), Sukinda, Orissa using pure culture of Aspergillus niger. Mineralogical analysis reveals a complete conversion of goethite into hematite phase leading to exposure of nickel particles into the micro-pores and cracks developed in the matrix which was initially found to be intertwined in the goethite lattice. As a result, it became more susceptible to attack by the fungal bio acids which in turn accelerate the dissolution rate. Organic acids like oxalic and citric acids were detected in the culture filtrate using HPLC. TEM analysis of the leached samples shows that nickel dissolute into the solution leaving a porous space in the matrix of the hematite by forming nickel oxalate or nickel citrate. Kinetics of the nickel bioleaching was studied to support the mechanism of the reaction. It was observed that the initial rate of reaction follows the chemical control dissolution reaction where as the later part fits to shrinking core model. 18% of nickel and 37.8% of cobalt was recovered from pre-treated COB at 2.5% pulp-density with 10% (v/v) fungal inoculum at 30 °C within 25 days in shake flask while 32.5% of nickel and 86% of cobalt was recovered in bioreactor. 相似文献
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57.
High irradiance and water stress induce alterations in pigment composition and chloroplast activities of primary wheat leaves 总被引:1,自引:0,他引:1
Rajendra Kumar Behera Prakash Chandra Mishra Nakul Kumar Choudhury 《Journal of plant physiology》2002,159(9)
Remarkable changes were observed in chlorophyll (Chl) (a+b), carotenoids (Car), and protein content of leaves and fluorescence emission, polarisation, excitation energy transfer, lipid peroxidation and DCPIP photoreduction activity in isolated chloroplasts of wheat leaves grown under moderate irradiance (MI, 15 W m−2, control) and subsequently exposed to high irradiance stress (HIS, 250 W m−2), water stress (WS, 5 % aqueous polyethylene glycol-4000 solution) and HIS+WS simultaneously, during mature and senescence phase. In the stress exposed samples the Chl, Car and protein contents and kinetics of Hill activity significantly declined. Decrease in excitation energy transfer and increase in membrane polarisation and accumulation of malondialdehyde (MDA) in chloroplasts were also observed. The effect was more pronounced when the seedlings were treated with HIS+WS simultaneously. These observations suggest additive and a possible synergetic action of HIS and WS causing faster loss of pigments and protein content, intense changes in membrane properties including photochemical function, compared to samples exposed to either of the stresses individually. 相似文献
58.
Results on the screening of species of the lichen family Graphidaceae for superoxide-scavenging activity (SSA) and xanthine-oxidase inhibitory (IXO) activity have been presented. The potential of the extracts for scavenging of superoxide and inhibition of xanthine-oxidase under various physiological conditions has been evaluated. The methanolic extracts of the species of family Graphidaceae showed inhibitory properties of xanthine oxidase (IC50 = 2.0 to 5.26 microg/ml) with an additional superoxide scavenging capacity (IC50 = 3.63 to 13.88 microg/ml). The potential of the methanolic extracts for scavenging of superoxide and inhibition of xanthine oxidase remained stable at 4 degrees C. Thus the extracts can be maintained for longer periods for their therapeutic uses. 相似文献
59.
Anit K. Behera Kelsey J. Schlund Allen J. Mason Kennedy O. Alila Mengyu Han Rebecca L. Grout Dana A. Baum 《Biopolymers》2013,99(6):382-391
Deoxyribozyme and aptamer selections are typically conducted in aqueous buffer solutions. Using nonaqueous cosolvents in selection experiments will help expand the activity of deoxyribozymes with non‐oligonucleotide substrates and will allow identification of new aptamers for nonprotein targets. We undertook in vitro selections utilizing a small amount of methanol in the reaction to keep the herbicides alachlor and atrazine in solution with the goal of identifying deoxyribozymes that require these herbicides for activity. The resulting deoxyribozymes successfully catalyze RNA ligation, but do not require alachlor or atrazine. Surprisingly, some of these deoxyribozymes displayed better catalytic activity in the presence of methanol over just aqueous buffer. We investigated several organic cosolvents to see if this enhancement was limited to methanol and found that other cosolvents, including ethanol, DMSO, and DMF, supported activity; in some cases, greater enhancement was observed. On the basis of these results, we tested two other previously identified RNA‐ligating deoxyribozymes to assess their tolerance of cosolvents and determined that different deoxyribozymes showed different responses to the cosolvents. Our results demonstrate that deoxyribozymes can tolerate and, in some cases, display enhanced activity in alternative solvent conditions. These findings will facilitate the development of responsive deoxyribozyme systems utilizing components with limited water solubility. © 2012 Wiley Periodicals, Inc. Biopolymers 99: 382–391, 2013. 相似文献
60.
Yuankun Zhai Jyotirmaya Behera Suresh C. Tyagi Neetu Tyagi 《Journal of cellular physiology》2019,234(10):18602-18614
Homocysteine (Hcy) is detrimental to bone health in a mouse model of diet-induced hyperhomocysteinemia (HHcy). However, little is known about Hcy-mediated osteoblast dysfunction via mitochondrial oxidative damage. Hydrogen sulfide (H2S) has potent antioxidant, anti-inflammatory, and antiapoptotic effects. In this study, we hypothesized that the H2S mediated recovery of osteoblast dysfunction by maintaining mitochondrial biogenesis in Hcy-treated osteoblast cultures in vitro. MC3T3-E1 osteoblastic cells were exposed to Hcy treatment in the presence or absence of an H2S donor (NaHS). Cell viability, osteogenic differentiation, reactive oxygen species (ROS) production were determined. Mitochondrial DNA copy number, adenosine triphosphate (ATP) production, and oxygen consumption were also measured. Our results demonstrated that administration of Hcy increases the intracellular Hcy level and decreases intracellular H2S level and expression of the cystathionine β-synthase/Cystathionine γ-lyase system, thereby inhibiting osteogenic differentiation. Pretreatment with NaHS attenuated Hcy-induced mitochondrial toxicity (production of total ROS and mito-ROS, ratio of mitochondrial fission (DRP-1)/fusion (Mfn-2)) and restored ATP production and mitochondrial DNA copy numbers as well as oxygen consumption in the osteoblast as compared with the control, indicating its protective effects against Hcy-induced mitochondrial toxicity. In addition, NaHS also decreased the release of cytochrome c from the mitochondria to the cytosol, which induces cell apoptosis. Finally, flow cytometry confirmed that NaHS can rescue cells from apoptosis induced by Hcy. Our studies strongly suggest that NaHS has beneficial effects on mitochondrial toxicity, and could be developed as a potential therapeutic agent against HHcy-induced mitochondrial dysfunction in cultured osteoblasts in vitro. 相似文献