Occurrences of rare cyanobacteria
Anabaena reniformis Lemmerm. and
Aphanizomenon aphanizomenoides (Forti) Horecká et Komárek were recently detected at several localities in the Czech Republic. Two monoclonal strains of
An. reniformis and one strain of
Aph. aphanizomenoides were isolated from distant localities and different sampling years. They were characterized by a combination of morphological, genetic, and biochemical approaches. For the first time, partial 16S rRNA gene sequences were obtained for these morphospecies. Based on this gene, all of these strains clustered separately from other planktonic
Anabaena and
Aphanizomenon strains. They appeared in a cluster with
Cylindrospermopsis Seenaya et Subba Raju and
Raphidiopsis F. E. Fritsch et M. F. Rich, clustered closely together with two
An. kisseleviana Elenkin strains available from GenBank. A new generic entity was defined (
Sphaerospermum gen. nov., with the type species
S. reniforme, based on the traditional species
An. reniformis). These results contribute significantly to the knowledge base about genetic heterogeneity among planktonic
Anabaena–like and
Aphanizomenon–like morphospecies. Accordingly, the subgenus
Dolichospermum, previously proposed for the group of planktonic
Anabaena, should be revaluated. Secondary metabolite profiles of the
An. reniformis and
Aph. aphanizomenoides strains differed considerably from 17 other planktonic
Anabaena strains of eight morphospecies isolated from Czech water bodies. Production of puwainaphycin A was found in both of the
An. reniformis strains. Despite the relatively short phylogenetic distance from
Cylidrospermopsis, the production of cylindrospermopsin was not detected in any of our strains.
相似文献