全文获取类型
收费全文 | 2520篇 |
免费 | 241篇 |
国内免费 | 5篇 |
专业分类
2766篇 |
出版年
2024年 | 1篇 |
2023年 | 23篇 |
2022年 | 37篇 |
2021年 | 56篇 |
2020年 | 24篇 |
2019年 | 57篇 |
2018年 | 59篇 |
2017年 | 63篇 |
2016年 | 105篇 |
2015年 | 182篇 |
2014年 | 192篇 |
2013年 | 197篇 |
2012年 | 262篇 |
2011年 | 234篇 |
2010年 | 150篇 |
2009年 | 127篇 |
2008年 | 175篇 |
2007年 | 169篇 |
2006年 | 147篇 |
2005年 | 125篇 |
2004年 | 93篇 |
2003年 | 92篇 |
2002年 | 88篇 |
2001年 | 16篇 |
2000年 | 10篇 |
1999年 | 10篇 |
1998年 | 24篇 |
1997年 | 5篇 |
1996年 | 4篇 |
1995年 | 9篇 |
1994年 | 7篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 6篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1974年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有2766条查询结果,搜索用时 10 毫秒
221.
Toll-like receptors 2 and 4 activate STAT1 serine phosphorylation by distinct mechanisms in macrophages 总被引:6,自引:0,他引:6
Rhee SH Jones BW Toshchakov V Vogel SN Fenton MJ 《The Journal of biological chemistry》2003,278(25):22506-22512
222.
Bezabeh T Mowat MR Jarolim L Greenberg AH Smith IC 《Cell death and differentiation》2001,8(3):219-224
Apoptosis and necrosis need to be differentiated in order to distinguish drug-induced cell death from spontaneous cell death due to hypoxia. The ability to differentiate between these two modes of cell death, especially at an early stage in the process, could have a significant impact on accessing the outcome of anticancer drug therapy in the clinic. Nuclear magnetic resonance spectroscopy was used to distinguish apoptosis from necrosis in human cervical carcinoma (HeLa) cells. Apoptosis was induced by treatment with the topoisomerase II inhibitor etoposide, whereas necrosis was induced by the use of ethacrynic acid or cytochalasin B. We found that the intensity of the methylene resonance increases significantly as early as 6 h after the onset of apoptosis, but that no such changes occur during necrosis. The spectral intensity ratio of the methylene to methyl resonances also shows a high correlation with the percentage of apoptotic cells in the sample (r2=0.965, P<0.003). 相似文献
223.
224.
The legume Rhizobium symbiosis leads to the formation of a new compartment in the plant cell, the symbiosome. This compartment harbours the bacteroids surrounded by a peribacteroid membrane (PBM) originating from the plant plasma membrane. The PBM and the space between the PBM and the bacteroid membrane, called peribacteroid space (PS), mediate the exchange of metabolites between the symbionts. Proteome analysis was used as an approach to characterise the proteins in the PBM and the PS. A standard differential centrifugation procedure including a Percoll gradient was used for symbiosome isolation from pea root nodules. Proteins in the PBM and PS fractions obtained from the symbiosomes were separated by two-dimensional gel electrophoresis, and 89 spots were analysed by tandem mass spectrometry. The proteins of 46 spots could be identified by database search. The results showed that PS and even PBM preparations from pea symbiosomes always contain abundant amounts of bacteroid proteins as a contaminate. Interestingly, in addition to a few PS/PBM proteins a number of endomembrane proteins (less likely representing a contaminate), including V-ATPase, BIP, and an integral membrane protein known from COPI-coated vesicles, were found in the PBM fraction, supporting the role of the endomembrane system in PBM biogenesis. 相似文献
225.
226.
227.
228.
Storre J Schäfer A Reichert N Barbero JL Hauser S Eilers M Gaubatz S 《The Journal of biological chemistry》2005,280(50):41380-41386
229.
Redemann S Schloissnig S Ernst S Pozniakowsky A Ayloo S Hyman AA Bringmann H 《Nature methods》2011,8(3):250-252
We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versions of any C. elegans gene can be designed using our web tool, C. elegans codon adapter. 相似文献
230.
Ann-Christin E. Brehler Wolfgang Hartmann Stefanie Wiebe Andrea Kerkhoff Christoph Schliemann Daniel Palmes Norbert Senninger Frank Lenze Hansjoerg Ullerich Wolfgang E. Berdel Torsten Kessler 《PloS one》2015,10(4)