全文获取类型
收费全文 | 295篇 |
免费 | 11篇 |
出版年
2021年 | 5篇 |
2019年 | 2篇 |
2018年 | 5篇 |
2017年 | 3篇 |
2016年 | 8篇 |
2015年 | 6篇 |
2014年 | 9篇 |
2013年 | 15篇 |
2012年 | 21篇 |
2011年 | 15篇 |
2010年 | 16篇 |
2009年 | 7篇 |
2008年 | 12篇 |
2007年 | 5篇 |
2006年 | 21篇 |
2005年 | 14篇 |
2004年 | 16篇 |
2003年 | 7篇 |
2002年 | 12篇 |
2001年 | 3篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 5篇 |
1987年 | 6篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1984年 | 6篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 11篇 |
1980年 | 5篇 |
1979年 | 6篇 |
1978年 | 7篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1973年 | 1篇 |
1971年 | 2篇 |
1970年 | 1篇 |
1968年 | 2篇 |
1965年 | 1篇 |
1959年 | 2篇 |
排序方式: 共有306条查询结果,搜索用时 15 毫秒
61.
Premise of the Study
The pygmy forest, a plant community of severely stunted conifers and ericaceous angiosperms, occurs on patches of highly acidic, nutrient‐poor soils along the coast of Northern California, USA. This system is an excellent opportunity to study the effect of severe nutrient deficiency on leaf physiology in a naturally‐occurring ecosystem. In this study, we seek to understand the physiological mechanisms stunting the plants' growth and their implications for whole plant function.Methods
We measured 14 traits pertaining to leaf photosynthetic function or physical structure on seven species. Samples were taken from the pygmy forest community and from conspecifics growing on higher‐nutrient soils, where trees may grow over 30 m tall.Key Results
Pygmy plants of most species maintained similar area‐based photosynthetic and stomatal conductance rates to conspecific controls, but had lower specific leaf area (leaf area divided by dry weight), lower percent nitrogen, and less leaf area relative to xylem growth. Sequoia sempervirens, a species rare in the pygmy forest, had a categorically different response from the more common plants and had remarkably low photosynthetic rates.Conclusions
Pygmy plants were not stunted by low photosynthetic rates on a leaf‐area basis; instead, several species had restricted whole‐plant photosynthesis due to low leaf area production. Pygmy plants of all species showed signs of greater carbon investment in their leaves and higher production of nonphotosynthetic leaf tissue, further contributing to slow growth rates. 相似文献62.
63.
Martina Boháčová Kamila Zdeňková Zuzana Tomáštíková Viviana Fuchsová Kateřina Demnerová Renáta Karpíšková Jarmila Pazlarová 《Folia microbiologica》2018,63(5):653-664
The alarming occurrence of antibiotic resistance genes in food production demands continuous monitoring worldwide. One reservoir of resistance genes is thought to be eDNA. There is currently little available information in Europe about either the extracellular DNA distribution of the bacterium or the spread of resistance genes in L. monocytogenes. Therefore, our aims were to give insight into the Listeria monocytogenes resistance situation in the Czech Republic and assess the presence of resistance genes in their extracellular DNA (eDNA). First, susceptibility tests were performed on 49 isolates of L. monocytogenes with selected antibiotics. Next, we tested DNA of suspected isolates for the presence of resistance genes in both planktonic cells and the eDNA of biofilms. Finally, fluorescent confocal microscopy was used to observe the eDNA pattern of selected isolates under conditions that mimicked the food processing environment and the human body. Susceptibility tests found isolates intermediate resistant to chloramphenicol, tetracycline, and ciprofloxacin as well as isolates resistant to ciprofloxacin. For all suspected isolates, PCR confirmed the presence of the gene lde encoding efflux pump in both types of DNA. When the biofilm was observed using confocal laser scanning microscope, the eDNA distribution patterns varied considerably according to the culture conditions. Furthermore, the food and clinical isolates varied in terms of the amount of eDNA detected. The presence of an efflux pump in both types of DNA suggests that the eDNA might serve as a reservoir of resistance genes. Surprising differences were observed in the eDNA pattern. Our results suggest that the current risk of the spread of L. monocytogenes resistance genes is low in the Czech Republic, but they also indicate the need for continuous long-term monitoring of the situation. 相似文献
64.
Campylobacter jejuni is responsible for the most common bacterial foodborne gastroenteritis. Despite its fastidious growth, it can survive harsh conditions through biofilm formation. In this work, fluorescence lectin-binding analysis was used to determine the glycoconjugates present in the biofilm matrix of two well-described strains. Screening of 72 lectins revealed strain-specific patterns with six lectins interacting with the biofilm matrix of both strains. The most common sugar moiety contained galactose and N-acetylgalactosamine. Several lectins interacted with N-acetylglucosamine and sialic acid, probably originated from the capsular polysaccharides, lipooligosaccharides and N-glycans of C. jejuni. In addition, glycoconjugates containing mannose and fucose were detected within the biofilm, which have not previously been found in the C. jejuni envelope. Detection of thioflavin T and curcumin highlighted the presence of amyloids in the cell envelope without association with specific cell appendages. The lectins ECA, GS-I, HMA and LEA constitute a reliable cocktail to detect the biofilm matrix of C. jejuni. 相似文献
65.
66.
Jarmila Kubíková 《Folia Geobotanica》1971,6(4):389-405
The gradients of floristic composition and environmental factors (temperature, soil moisture, pH, light intensity) were followed on three transects delimited in the contact zone between woodland and xerothermic grassland on the NW. slope of the Velká hora Hill in the Bohemian Karst. The densities of the more frequent plants and corresponding values of environmental factors are presented in graph form. Three different ecotopes, e.g. woodland, thermophilous grassland, contact between woodland and grassland, could be traced. The values of temperature, soil moisture, light intensity grouped according to the three ecotopes show significant differences in the respective mean. Furthemore, the association between species has been calculated and thechi-square matrix for the 11 most frequent species is given. On the basis of these calculations, a diagram of assumed relations between species was constructed. On the ground of data obtained, a significant regression of the density of thermophilous herbaceous plants on the temperature of the soil surface was found. The type of gradient on this experimental site is discussed and noticeable step-like, discontinuous character of both the floristic and environmental gradient is shown. 相似文献
67.
68.
69.
70.