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201.
S. R. Schmolke K. Broeg S. Zander V. Bissinger P. D. Hansen N. Kress B. Herut E. Jantzen G. Krüner A. Sturm W. Körting H. von Westernhagen 《Helgoland Marine Research》1999,53(3-4):257-266
A comprehensive database, containing biological and chemical information, collected in the framework of the bilateral interdisciplinary
MARS project (”biological indicators of natural and man-made changes in marine and coastal waters”) during the years 1995–1997
in the coastal environment of the North Sea, was subjected to a multivariate statistical evaluation. The MARS project was
designated to combine a variety of approaches and to develop a set of methods for the employment of biological indicators
in pollution monitoring and environmental quality assessment. In total, nine ship cruises to four coastal sampling sites were
conducted; 765 fish and 384 mussel samples were analysed for biological and chemical parameters. Additional information on
the chemical background at the sampling sites was derived from sediment samples, collected at each of the four sampling sites.
Based on the available chemical data in sediments and black mussel (Mytilus edulis) a pollution gradient between the selected sites, was established. The chemical body burden of flounder (Platichthys flesus) from these sites, though, did not reflect this gradient equally clear. In contrast, the biological information derived from
measurements in fish samples displayed significant a regional as well as a temporal pattern. A multivariate bioindicator data
matrix was evaluated employing a factor analysis model to identify relations between selected biological indicators, and to
improve the understanding of a regional and temporal component in the parameter response. In a second approach, applying the
k-means algorithm on the data matrix, two significantly different clusters of samples, characterised by the current health
status of the fish, were extracted. Using this classification a temporal, and in the second order, a less pronounced spatial
effect was evident. In particular, during July 1996, a clear sign of deteriorating environmental conditions was extracted
from the biological data matrix.
Received: 20 June 1999 / Received in revised form: 8 November 1999 / Accepted: 8 November 1999 相似文献
202.
D Homann T Dyrberg J Petersen M B Oldstone M G von Herrath 《Journal of immunology (Baltimore, Md. : 1950)》1999,163(4):1833-1838
Oral administration of self-Ags can dampen or prevent autoimmune processes by induction of bystander suppression. Based on encouraging results from experiments in nonobese diabetic (NOD) mice, clinical trials have been initiated in type 1 diabetes using human insulin as an oral Ag. However, neither the precise antigenic requirements nor the mechanism of bystander suppression are currently understood in detail. Here we report that 1) a 1-aa difference in position 30 of the insulin B chain abrogated the ability of insulin to confer protection in both NOD as well as a virus-induced transgenic mouse model for type 1 diabetes. In the latter model transgenic mice express the nucleoprotein (NP) of lymphocytic choriomeningitis virus (LCMV) under the control of the rat insulin promotor (RIP) in the pancreatic beta cells and develop diabetes only following LCMV infection; and 2) protection could be transferred with insulin B chain-restimulated but not LCMV-restimulated splenocytes from RIP-NP transgenic mice, demonstrating that the mechanism of diabetes prevention in the RIP-NP model is mediated by insulin B chain-specific, IL-4-producing regulatory cells acting as bystander suppressors. 相似文献
203.
Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases 总被引:30,自引:0,他引:30
Opresko PL von Kobbe C Laine JP Harrigan J Hickson ID Bohr VA 《The Journal of biological chemistry》2002,277(43):41110-41119
Werner syndrome is a human premature aging disorder displaying cellular defects associated with telomere maintenance including genomic instability, premature senescence, and accelerated telomere erosion. The yeast homologue of the Werner protein (WRN), Sgs1, is required for recombination-mediated lengthening of telomeres in telomerase-deficient cells. In human cells, we report that WRN co-localizes and physically interacts with the critical telomere maintenance protein TRF2. This interaction is mediated by the RecQ conserved C-terminal region of WRN. In vitro, TRF2 demonstrates high affinity for WRN and for another RecQ family member, the Bloom syndrome protein (BLM). TRF2 interaction with either WRN or BLM results in a notable stimulation of their helicase activities. Furthermore, the WRN and BLM helicases, partnered with replication protein A, actively unwind long telomeric duplex regions that are pre-bound by TRF2. These results suggest that TRF2 functions with WRN, and possibly BLM, in a common pathway at telomeric ends. 相似文献
204.
Features of Crassulacean acid metabolism (CAM) were studied in a variety of different succulents in response to climatic conditions between March 1977 and October 1983 in the southern Namib desert (Richtersveld). A screening in 1977 and 1978 revealed that nearly all investigated succulents performed a CAM, but overnight accumulation of malate declined gradually with decreasing soil water potential, tissue osmotic potential, and leaf water content. This was further substantiated by an extended period of insufficient rainfall in 1979 and 1980 which damaged the evergreen CAM succulents between 80 and 100%. In most of the species still living, neither CO2-gas exchange nor diurnal acid fluctuation, indicative of CAM, could be detected unless an abundant rainfall restored both CAM features. Plants persisted in a stage of latent life.Water supply is one necessary prerequisite for CAM in the Richtersveld. But even well-watered plants with CAM were sensitive to short-term water stress caused by high water-vapour partialpressure deficit (VPD) in the night, which reduced or prevented CO2 uptake and resulted in a linear relation between overnight accumulated malate and VPD. The results do not support the opinion that, for the Namib succulents, CAM is an adaptive mechanism to water stress since long-term and short-term water stress stopped nocturnal malate synthesis, but instead lead to the conclusion that nocuturnal CO2 fixation is only performed when the water status of the plant can be improved simultaneously.Abbreviations CAM
Crassulacean acid metabolism
- VPD
water vapour pressure deficit
Dedicated to Professor H. Ziegler on the occasion of his 60th birthday 相似文献
205.
Anja von Nahmen Andreas Post Hans-Joachim Galla M. Sieber 《European biophysics journal : EBJ》1997,26(5):359-369
Three compounds of the pulmonary surfactant – dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG),
and the surfactant associated protein C (SP-C) – were spread at the air-water interface of a Langmuir trough as a model system
to mimic the properties of natural surfactant. Fluorescence microscopical images of the film formed at the interface were
obtained during compression using a fluorescence dye bound covalently either to phosphatidylcholine or to SP-C. The images
were quantified using statistical methods in respect to relative areas and relative fluorescence intensities of the domains
found. In the early stage of compression, film pressure rose slightly and was accompanied by a phase separation which could
be recognized in the images by the formation of bright and dark domains. On further compression, after a steep increase of
film pressure, a plateau region of constant film pressure started abruptly. During compression in the plateau region, fluorescence
intensity of the bright domain formed in the early stage of compression increased. The increasing fluorescence intensity,
the non-Gaussian intensity distribution of the bright domain, and the small mean molecular area of the film in the plateau
region gave rise to the assumption that multilayer structures were formed in the late stage of compression. The formation
of the multilayer structures was fully reversible in repeated compression-expansion cycles including the plateau region of
the phase diagram. The ability of lipid/SP-C mixtures to form reversible multilayer structures during compression may be relevant
to stability in lungs during expiration and inhalation.
Received: 13 February 1997 / Accepted: 22 May 1997 相似文献
206.
M I Al-Sheikhly H P Schuchmann C von Sonntag 《International journal of radiation biology and related studies in physics, chemistry, and medicine》1985,47(4):457-462
In the radiolysis of aqueous formate-containing solutions a chain reaction (i, ii) proceeds in the presence of N2O. CO2-. + N2O + H2O----CO2 + N2 + .OH + OH- (i) .OH + HCO2-.----CO2-. + H2O (ii) The chain length depends on the dose rate and the N2O concentration but not on the formate concentration. Typically, G(CO2) approximately 140 molecules (100 eV)-1 is found, with an equivalent amount of N2, at a dose rate of 3 X 10(-3) Gy s-1. The rate constant for the rate-determining step in this chain reaction has been calculated at k(i) = 1600 dm3 mol-1 s-1. The possible relevance of this chain reaction in radiation biological studies is briefly discussed. 相似文献
207.
The results of genome analysis of five hybrids, viz.Elymus patagonicus ×Hordeum procerum, E. patagonicus ×H. tetraploidum, E. angulatus ×H. jubatum, E. angulatus ×H. lechleri, andE. angulatus ×H. parodii, are reported. The genomic constitution ofHordeum tetraploidum andH. jubatum is best given as H1H1H2H2, ofH. lechleri andH. parodii as H1H1H2H2H4H4, ofH. procerum as H1H1H2H2H3H3, and ofElymus patagonicus andE. angulatus as SSH1H1H2H2. 相似文献
208.
Ralph Schröder Anke Maassen Andrea Lippoldt Thomas Börner Rüdiger von Baehr Peter Dobrowolski 《Applied microbiology and biotechnology》1991,35(5):631-637
Summary Using the broad-host-range promoter probe vector pRS201 for cloning of phage Acm1 promoters, we established a convenient vector system for expression of heterologous genes in different Gram-negative bacteria. The usefulness of this system was demonstrated by expression of the HBV core gene in Acetobacter methanolicus. Plasmids carrying the HBV core gene downstream of different Acm1-phage promoters were transferred to A. methanolicus, a new potential host for recombinant DNA expression. Using enzyme immunoassay and immunoblot techniques, the amount and composition of core antigen produced in A. methanolicus were compared with that derived from Escherichia coli. The expression of immunoreactive core antigen in A. methanolicus exceeds by sevenfold that in E. coli using an expression system with tandemly arranged promoters. Morphological observations by electron microscopy show that the HBV core gene products isolated from both hosts are assembled into regular spherical particles with a diameter of about 28 nm that are comparable to original viral nucleocapsids.
Offprint requests to: R. Schröder 相似文献
209.
Maria Troullinaki Vasileia‐Ismini Alexaki Ioannis Mitroulis Anke Witt Anne Klotzschevon Ameln Kyoung‐Jin Chung Triantafyllos Chavakis Matina Economopoulou 《Journal of cellular and molecular medicine》2019,23(4):2362-2371
The mechanism underlying vasoproliferative retinopathies like retinopathy of prematurity (ROP) is hypoxia‐triggered neovascularisation. Nerve growth factor (NGF), a neurotrophin supporting survival and differentiation of neuronal cells may also regulate endothelial cell functions. Here we studied the role of NGF in pathological retinal angiogenesis in the course of the ROP mouse model. Topical application of NGF enhanced while intraocular injections of anti‐NGF neutralizing antibody reduced pathological retinal vascularization in mice subjected to the ROP model. The pro‐angiogenic effect of NGF in the retina was mediated by inhibition of retinal endothelial cell apoptosis. In vitro, NGF decreased the intrinsic (mitochondria‐dependent) apoptosis in hypoxia‐treated human retinal microvascular endothelial cells and preserved the mitochondrial membrane potential. The anti‐apoptotic effect of NGF was associated with increased BCL2 and reduced BAX, as well as with enhanced ERK and AKT phosphorylation, and was abolished by inhibition of the AKT pathway. Our findings reveal an anti‐apoptotic role of NGF in the hypoxic retinal endothelium, which is involved in promoting pathological retinal vascularization, thereby pointing to NGF as a potential target for proliferative retinopathies. 相似文献
210.