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61.
Clearing of native vegetation for agriculture has left 1.047 million hectares of southwest Western Australia affected by dryland
salinity, and this area may expand up to a further 1.7–3.4 million hectares if trends continue. Ecosystems in saline-affected
regions display many of the classic characteristics of Ecosystem Distress Syndrome, one outcome of which has not yet been
investigated in relation to dryland salinity: adverse human health implications. This article seeks to review existing information
and identify potential adverse human health effects. Three key potential impacts on human health resulting from dryland salinity
are identified: wind-borne dust and respiratory health; altered ecology of the mosquito-borne disease Ross River virus; and
mental health consequences of salinity-induced environmental degradation. Given the predicted increase in extent and severity
of dryland salinity over coming decades, adverse outcomes of salinity are likely to be further exacerbated, including those
related to human health. There is a clear need to investigate the issues discussed in this review and also to identify other
potential adverse health effects of dryland salinity. Investigations must be multidisciplinary to sufficiently examine the
broad scope of these issues. The relationship between human health and salinity may also be relevant beyond Australia in other
countries where secondary soil salinization is occurring. 相似文献
62.
Jardine CM Janecko N Allan M Boerlin P Chalmers G Kozak G McEwen SA Reid-Smith RJ 《Applied and environmental microbiology》2012,78(11):3873-3879
We conducted a cross-sectional study to determine the prevalence of antimicrobial resistance (AMR) in fecal Escherichia coli isolates from raccoons (Procyon lotor) living in Ontario, Canada. From June to October 2007, we trapped raccoons in three areas: one primarily urban site around Niagara, one primarily rural site north of Guelph, and one at the Toronto Zoo. In addition, we conducted a longitudinal study at the Toronto Zoo site to investigate the temporal dynamics of fecal E. coli and AMR in raccoons. Reduced susceptibility to ≥1 antimicrobial agent was detected in E. coli isolates from 19% of 16 raccoons at the urban site, 17% of 29 raccoons from the rural site, and 42% of 130 samples collected from 59 raccoons at the zoo site. Raccoons from the zoo site were significantly more likely to shed E. coli with reduced susceptibility to ≥1 antimicrobial agent than animals from the rural site (odds ratio [OR], 3.41; 95% confidence interval [CI], 1.17 to 12.09; P = 0.02). Resistance to expanded-spectrum cephalosporins (and the associated bla(CMY-2) gene) was detected in two animals from the zoo site and one animal from the rural site. Serotyping and pulsed-field gel electrophoresis analysis show that raccoons on the zoo grounds harbor a diverse assemblage of E. coli, with rapid bacterial turnover within individuals over time. Our study indicates that raccoons may shed resistant bacteria of public health significance and that raccoons have the potential to disseminate these bacteria throughout their environment. 相似文献
63.
64.
Kumar K Jenkins JL Jardine AM Shapiro R 《Biochemical and biophysical research communications》2003,300(1):81-86
The most potent low molecular weight inhibitors of pancreatic RNase superfamily enzymes reported to date are synthetic derivatives of adenosine 5(')-pyrophosphate. Here we have investigated the effects of six natural nucleotides that also incorporate this moiety (NADP(+), NADPH, ATP, Ap(3)A, Ap(4)A, and Ap(5)A) on the activities of RNase A and two of its homologues, eosinophil-derived neurotoxin and angiogenin. With eosinophil-derived neurotoxin and angiogenin, Ap(5)A is comparable to the tightest binding inhibitors identified previously (K(i) values at pH 5.9 are 370 nM and 100 microM, respectively); it ranks among the strongest small antagonists of RNase A as well (K(i)=230 nM). The K(i) for NADPH with angiogenin is similar to that of Ap(5)A. These findings suggest that Ap(5)A and NADPH may serve as useful new leads for inhibitor design. Examination of inhibition under physiological conditions indicates that NADPH, ATP, and Ap(5)A may suppress intracellular RNase activity significantly in vivo. 相似文献
65.
McBurney MW Yang X Jardine K Bieman M Th'ng J Lemieux M 《Molecular cancer research : MCR》2003,1(5):402-409
The yeast sir2 gene plays a central role in mediating gene silencing and DNA repair in this organism. The mouse sir2alpha gene is closely related to its yeast homologue and encodes a nuclear protein expressed at particularly high levels in embryonic stem (ES) cells. We used homologous recombination to create ES cells null for sir2alpha and found that these cells did not have elevated levels of acetylated histones and did not ectopically express silent genes. Unlike yeast sir2 mutants, our sir2alpha null ES cells had normal sensitivity to insults such as ionizing radiation and heat shock, and they were able to silence invading retroviruses normally. These sir2alpha null cells were able to differentiate in culture normally. Our results failed to provide evidence that the mammalian SIR2alpha protein plays a role in gene silencing and suggest that the physiological substrate(s) for the SIR2alpha deacetylase may be nuclear proteins other than histones. 相似文献
66.
Jardine MA Spies HS Nkambule CM Gammon DW Steenkamp DJ 《Bioorganic & medicinal chemistry》2002,10(4):875-881
Members of the actinomycetes produce 1D-1-O-(2-[N-acetyl-L-cysteinyl]amino-2-deoxy-alpha-D-glucopyranosyl)-myo-inositol or mycothiol 1 as principal low molecular mass thiol. Chemical synthesis of a biosynthetic precursor of mycothiol, the pseudodisaccharide 1D-1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-myo-inositol 13 was achieved by the following steps: (1) Enantioselective synthesis gave the glycosyl acceptors (-)-2,3,4,5,6-penta-O-acetyl-D-myo-inositol D-7 and the corresponding L-isomer L-7. (2) Condensation of D-7 and L-7 with the glycosyl donor 3,4,6-tri-O-acetyl-2-deoxy-2-(2,4-dinitrophenylamino)-alpha-D-glucopyranosylbromide afforded the corresponding alpha and beta anomeric products, which could be resolved by silica gel chromatography. (3) Deprotection of these by hydrolysis using an anion exchange resin gave 1D- and 1L-1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-myo-inositol 13 and 15 and the corresponding beta-coupled anomers 14 and 16. Only 13, and to a much lesser extent 15, were used by enzymes present in an ammonium sulphate fraction of a cellfree extract of Mycobacterium smegmatis for the enzymatic synthesis of mycothiol. In the absence of acetyl-SCoA, the immediate biosynthetic precursor of 1, desacetylmycothiol, was the major product. 相似文献
67.
Adults of the human parasitic trematode Schistosoma mansoni, which causes
hepatosplenic/intestinal complications in humans, synthesize
glycoconjugates containing the Lewis x (Lex) Galbeta1-->4(Fucalpha1--
>3)GlcNAcbeta1-->R, but not sialyl Lewis x (sLex), antigen. We now
report on our analyses of Lexand sLexexpression in S.haematobium and
S.japonicum, which are two other major species of human schistosomes that
cause disease, and the possible autoimmunity to these antigens in infected
individuals. Antigen expression was evaluated by both ELISA and Western
blot analyses of detergent extracts of parasites using monoclonal
antibodies. Several high molecular weight glycoproteins in both S.
haematobium and S. japonicum contain the Lexantigen, but no sialyl
Lexantigen was detected. In addition, sera from humans and rodents infected
with S.haematobium and S.japonicum contain antibodies reactive with Lex.
These results led us to investigate whether Lexantigens are expressed in
other helminths, including the parasitic trematode Fasciola hepatica , the
parasitic nematode Dirofilaria immitis (dog heartworm), the ruminant
nematode Haemonchus contortus , and the free-living nematode Caenorhabditis
elegans . Neither Lexnor sialyl-Lexis detectable in these other helminths.
Furthermore, none of the helminths, including schistosomes, express Lea,
Leb, Ley, or the H- type 1 antigen. However, several glycoproteins from all
helminths analyzed are bound by Lotus tetragonolobus agglutinin , which
binds Fucalpha1-->3GlcNAc, and Wisteria floribunda agglutinin, which
binds GalNAcbeta1-->4GlcNAc (lacdiNAc or LDN). Thus, schistosomes may be
unique among helminths in expressing the Lexantigen, whereas many different
helminths may express alpha1,3-fucosylated glycans and the LDN motif.
相似文献
68.
A compound having fluorescence characteristics similar to those of 5-hydroxytryptamine (5-HT), when reacted with ninhydrin, is present in several invertebrates (Fasciola hepatica, Aplysia californica, Tritonia diomedia, and Hirudo medicinalis). However, this substance is not identical with 5-HT [ Andreini, G. C., Beretta, C., Faustini, R., and Gallina, G. (1970)Experientia26, 166–167]. We confirmed these findings for Fasciola and also observed this substance in Spirometra mansonoides and mouse brain. Using chromatography and amino acid analysis we identified this substance as lysine. While 5-HT is also demonstrable in Spirometra, no 5-HT is present in Fasciola. Although epinephrine and dopamine seem to be present in Spirometra when the hydroxyindole technique of Laverty and Taylor [ Laverty, R., and Taylor, K. M. (1968)Anal. Biochem.22, 269–279] is used, neither amine is detectable in adult Spirometra by mass spectrometric analysis. A relatively high concentration of tyrosine in Spirometra could account for the apparent presence of dopamine. Therefore, lysine, tyrosine, and possibly other substances can be sources of error in the detection of biogenic amines in invertebrates. 相似文献
69.
70.
Cunjak RA Roussel JM Gray MA Dietrich JP Cartwright DF Munkittrick KR Jardine TD 《Oecologia》2005,144(4):636-646
Information about animal movements has often been inferred from stable isotope analysis (SIA), but is dependent on animals
assimilating site-specific isotopic signatures via diet. This potential weakness in ecological interpretation can be overcome
by using other investigative tools that provide precise information about individual movement patterns. In this paper, we
demonstrate the value of combining SIA with telemetry or mark-recapture data from trapping, electrofishing and remote detection
of individuals to study the movement and feeding ecology of fishes in different habitats. In a fjord lake system in Newfoundland,
Canada, juvenile Atlantic salmon delayed downstream migration (smolts) or actively moved into a large lake (parr) where they
foraged for periods reflecting different life history strategies. In the Miramichi River (New Brunswick, Canada), SIA provided
evidence of distinct foraging habitats (tributary versus large river). By tracking fish implanted with passive integrated
transponder (PIT) tags, we distinguished between movements related to foraging versus seeking cool water refugia during high
temperature events. Finally, site fidelity and limited mobility of slimy sculpin, a small benthic fish, was established where
δ13C in muscle tissue showed a progressive enrichment downstream and where a median displacement of <10 m was estimated for sculpin
tagged with PIT tags. Technological improvements have permitted non-destructive tissue sampling of wild fishes for SIA, and
the tagging and remote detection of animals smaller than was previously possible. These advancements and the combination of
investigative tools promise new insights into animal ecology. 相似文献