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排序方式: 共有228条查询结果,搜索用时 15 毫秒
121.
Jardine DL Charles CJ Frampton CM Richards AM 《American journal of physiology. Heart and circulatory physiology》2007,293(1):H433-H439
The association between cardiac sympathetic nerve activity (CSNA) and ventricular fibrillation (VF) during acute myocardial infarction (MI) has not been assessed in conscious animal models. During the first 60 min post-MI, mean blood pressure (MBP), heart rate (HR), and CSNA were recorded continuously in 20 conscious sheep. Resistant sheep (group A, n = 10) were compared with susceptible sheep (group B, n = 10) who developed fatal VF (n = 7) or sustained ventricular tachycardia (VT, n = 3). The mean time to VF/VT was 28.1 +/- 3.3 min. In group B, MBP, HR, and CSNA were averaged at each consecutive minute from baseline at 14 min before the onset of VF/VT and compared with time-matched values in group A. When compared with those of group A, indexes of CSNA burst size increased before the onset of VF/VT: burst area/minute (F(13,208) = 2.17, P = 0.01) and burst area/100 beats (F(13,208) = 1.86, P = 0.04). By contrast, burst frequency indexes were not significantly different: burst frequency (F(13,208) = 1.6, P = 0.09) and burst incidence (F(13,208) = 1.48, P = 0.13). In group A, CSNA burst area/min and burst area/100 beats did not change across this time period (F(13,117) = 0.97, P = 0.5, F(13,117) = 0.96, P = 0.7) but increased with time in group B (F(13,91) = 2.3, P = 0.01; and F(13,91) = 2.25, P = 0.01). Between-group comparisons demonstrated no differences in time of onset of ventricular ectopic beats: 18.5 (range 12-24) in group A versus 15.0 min (range 7-22) in group B (Mann-Whitney U-test, P = 0.09). Pre-MI baroreflex slopes were similar: R-R slopes were 11.8 +/- 2 and 15.6 +/- 1.1 ms/mmHg (t(18) = -1.6, P = 0.14). CSNA slopes were -1.8 +/- 0.3 and -2.3 +/- 0.2%/mmHg (t(18) = -1.4, P = 0.2). An early increase in CSNA burst size indexes (before 60 min post-MI), mediated by an excitatory sympathetic reflex, is important in the genesis of VF/VT. 相似文献
122.
Michael D A Lindsay rew Jardine Cheryl A Johansen Anthony E Wright Susan A Harrington Philip Weinstein 《Australian Journal of Entomology》2007,46(1):60-64
Abstract Previously existing sources of data regarding mosquitoes in inland areas of south-west Western Australia are few and qualitative in nature. This is the first attempt to quantitatively investigate mosquito fauna in this region. The existing data are reviewed and compared with the results of the quantitative surveys presented in this paper. Temporal comparisons appear to indicate that mosquito community structure in the region may have changed since the initial surveys in the 1950s from a combination of freshwater-breeding species towards a strong dominance of Aedes camptorhynchus (Thomson), a major vector of Ross River virus in southern Australia. It is speculated that this shift may have been brought about by the increasing area and severity of dryland salinity in the region over the last century, and also may increase the potential for Ross River virus disease transmission. 相似文献
123.
Neshich G Mazoni I Oliveira SR Yamagishi ME Kuser-Falcão PR Borro LC Morita DU Souza KR Almeida GV Rodrigues DN Jardine JG Togawa RC Mancini AL Higa RH Cruz SA Vieira FD Santos EH Melo RC Santoro MM 《Genetics and molecular research : GMR》2006,5(4):717-722
Star STING is the latest version of the STING suite of programs and corresponding database. We report on five important aspects of this package that have acquired some new characteristics, designed to add key advantages to the whole suite: 1) availability for most popular platforms and browsers, 2) introduction of the STING_DB quality assessment, 3) improvement in algorithms for calculation of three STING parameters, 4) introduction of five new STING modules, and 5) expansion of the existing modules. Star STING is freely accessible at: http://sms.cbi.cnptia.embrapa.br/SMS/, http://trantor.bioc.columbia.edu/SMS, http://www.es.embnet.org/SMS/, http://gibk26.bse.kyutech.ac.jp/SMS/ and http://www.ar.embnet.org/SMS. 相似文献
124.
Joseph G. Jardine Devin Sok Jean-Philippe Julien Bryan Briney Anita Sarkar Chi-Hui Liang Erin M. Scherer Carole J. Henry Dunand Yumiko Adachi Diwanji Devan Jessica Hsueh Meaghan Jones Oleksandr Kalyuzhniy Michael Kubitz Skye Spencer Matthias Pauthner Karen L. Saye-Francisco Fabian Sesterhenn Patrick C. Wilson Denise A. Galloway Robyn L. Stanfield Ian A. Wilson Dennis R. Burton William R. Schief 《PLoS pathogens》2016,12(9)
125.
L. David Finger Nikesh Patel Amanda Beddows Long Ma Jack C. Exell Emma Jardine Anita C. Jones Jane A. Grasby 《Nucleic acids research》2013,41(21):9839-9847
The structure- and strand-specific phosphodiesterase flap endonuclease-1 (FEN1), the prototypical 5′-nuclease, catalyzes the essential removal of 5′-single-stranded flaps during replication and repair. FEN1 achieves this by selectively catalyzing hydrolysis one nucleotide into the duplex region of substrates, always targeting the 5′-strand. This specificity is proposed to arise by unpairing the 5′-end of duplex to permit the scissile phosphate diester to contact catalytic divalent metal ions. Providing the first direct evidence for this, we detected changes induced by human FEN1 (hFEN1) in the low-energy CD spectra and fluorescence lifetimes of 2-aminopurine in substrates and products that were indicative of unpairing. Divalent metal ions were essential for unpairing. However, although 5′-nuclease superfamily-conserved active-site residues K93 and R100 were required to produce unpaired product, they were not necessary to unpair substrates. Nevertheless, a unique arrangement of protein residues around the unpaired DNA was detected only with wild-type protein, suggesting a cooperative assembly of active-site residues that may be triggered by unpaired DNA. The general principles of FEN1 strand and reaction-site selection, which depend on the ability of juxtaposed divalent metal ions to unpair the end of duplex DNA, may also apply more widely to other structure- and strand-specific nucleases. 相似文献
126.
Joshua Pajak Erik Dill Emilio Reyes-Aldrete Mark A White Brian A Kelch Paul
J Jardine Gaurav Arya Marc
C Morais 《Nucleic acids research》2021,49(11):6474
Double-stranded DNA viruses package their genomes into pre-assembled capsids using virally-encoded ASCE ATPase ring motors. We present the first atomic-resolution crystal structure of a multimeric ring form of a viral dsDNA packaging motor, the ATPase of the asccφ28 phage, and characterize its atomic-level dynamics via long timescale molecular dynamics simulations. Based on these results, and previous single-molecule data and cryo-EM reconstruction of the homologous φ29 motor, we propose an overall packaging model that is driven by helical-to-planar transitions of the ring motor. These transitions are coordinated by inter-subunit interactions that regulate catalytic and force-generating events. Stepwise ATP binding to individual subunits increase their affinity for the helical DNA phosphate backbone, resulting in distortion away from the planar ring towards a helical configuration, inducing mechanical strain. Subsequent sequential hydrolysis events alleviate the accumulated mechanical strain, allowing a stepwise return of the motor to the planar conformation, translocating DNA in the process. This type of helical-to-planar mechanism could serve as a general framework for ring ATPases. 相似文献
127.
128.
129.
Cameron KO Beretta EE Chen Y Chu-Moyer M Fernando D Gao H Kohrt J Lavergne S Jardine Pda S Guzman-Perez A Hoth C Perry DA Hadcock JR Gautreau D Makowski M Perez S Polivkova J Rogers L Scott DO Swick AG Thiede L Trebino CE Trilles RV Wilmowski J Zhang Y 《Bioorganic & medicinal chemistry letters》2012,22(8):2943-2947
New cholecystokinin-1 receptor (CCK1R) agonist 'triggers' were identified using iterative library synthesis. Structural activity relationship studies led to the discovery of compound 10e, a potent CCK1R agonist that demonstrated robust weight loss in a diet-induced obese rat model with very low systemic exposure. Pharmacokinetic data suggest that efficacy is primarily driven through activation of CCK1R's located within the intestinal wall. 相似文献
130.
Jardine TD Pusey BJ Hamilton SK Pettit NE Davies PM Douglas MM Sinnamon V Halliday IA Bunn SE 《Oecologia》2012,168(3):829-838
High levels of hydrological connectivity during seasonal flooding provide significant opportunities for movements of fish
between rivers and their floodplains, estuaries and the sea, possibly mediating food web subsidies among habitats. To determine
the degree of utilisation of food sources from different habitats in a tropical river with a short floodplain inundation duration
(~2 months), stable isotope ratios in fishes and their available food were measured from three habitats (inundated floodplain,
dry season freshwater, coastal marine) in the lower reaches of the Mitchell River, Queensland (Australia). Floodplain food
sources constituted the majority of the diet of large-bodied fishes (barramundi Lates calcarifer, catfish Neoarius graeffei) captured on the floodplain in the wet season and for gonadal tissues of a common herbivorous fish (gizzard shad Nematalosa come), the latter suggesting that critical reproductive phases are fuelled by floodplain production. Floodplain food sources also
subsidised barramundi from the recreational fishery in adjacent coastal and estuarine areas, and the broader fish community
from a freshwater lagoon. These findings highlight the importance of the floodplain in supporting the production of large
fishes in spite of the episodic nature and relatively short duration of inundation compared to large river floodplains of
humid tropical regions. They also illustrate the high degree of food web connectivity mediated by mobile fish in this system
in the absence of human modification, and point to the potential consequences of water resource development that may reduce
or eliminate hydrological connectivity between the river and its floodplain. 相似文献