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41.
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The microelectrophoresis procedure on agarose devised by Jaques, Ballieux, Dietrich, and Kavanagh (1) for the identification and measurement of heparin provides a quick, versatile, and adaptable procedure. Differentiation of heparin and related polyanions can be accomplished at various stages of the procedure—migration, fixation, staining, destaining, evaluation. Tests such as solubility in solvents, precipitation by chemical reagents, action of enzymes, may be applied to minute amounts of materials.A linear relationship was observed between absorbance and heparin concentration applied and between log of spot area and log concentration so that total optical density of spots can be used to estimate heparin. Visual comparison of microelectrophoresis slides with a series of reference slides with 0.02 to 0.6 units of heparin gave a coefficient of variation of 15.5%. Difficulty was found in providing a densitometer with sufficient sensitivity and reproducibility. Special slide holders were designed to hold the microelectrophoresis slide in the Chromoscan densitometer and in the Beckman DK-2 spectrophotometer. By specifying carriage position for the slide, slit size in the optical system, baseline adjustment, background correction, light filter, reproducibility of measurement could be achieved with the Chromoscan densitometer with 10% maximum error with low counts. The Coefficient of Variation was 6.2% when all precautions were taken. 相似文献
43.
Jaques Belik Gema E. González-Luis Francisco Perez-Vizcaino Eduardo Villamor 《Free radical biology & medicine》2010,48(2):177-188
Isoprostanes are prostaglandin-like bioactive molecules generated via nonenzymatic peroxidation of lipid membrane-derived arachidonic acid by free radicals and reactive oxygen species. Their cognate receptors, biological actions, and signaling pathways are poorly understood. Aside from being sensitive and specific biomarkers of oxidative stress, E- and F-ring isoprostanes have important biological functions and likely mediate many of the disease-related pathological changes for which they are used as indicators. The biochemical pathways involved in isoprostane formation, their pathogenetic relevance to adult disease states, and their biological function are addressed. Developmentally, plasma and tissue content data show that isoprostane levels are highest during fetal and early neonatal life, when compared with adults. As such, the available data suggesting that isoprostanes play an important biological role, as well as possibly actively participate in the regulation of pulmonary vascular tone and the transition from fetal to postnatal life, are here reviewed. Lastly, the association between isoprostanes and certain neonatal clinical conditions is addressed. Although its existence has been recognized for almost 20 years, little is known about the critical importance of isoprostanes during fetal life and immediate neonatal period. This review is an attempt to bridge this knowledge gap. 相似文献
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45.
A modified method for the colorimetric determination of heparin 总被引:5,自引:0,他引:5
46.
Nitric oxide level, protein oxidation and antioxidant enzymes in rats infected by Trypanosoma evansi
AS Da Silva FC Paim RC Santos MB Sangoi RN Moresco ST Lopes JA Jaques J Baldissarelli VM Morsch SG Monteiro 《Experimental parasitology》2012,130(2):166-170
The acaricidal (miticidal) activity of 90% ethanolic extracts of leaves and stem bark of Swietenia mahogani and Swietenia macrophylla were tested against Varroa destructor mite. Four concentrations were used over two different time intervals under laboratory and field conditions. In general, it was noticed that the acaricidal effect based on mortality and LC(50) of all tested extracts against the Varroa mite was concentration and time dependant. The acaricidal action against Varroa mites was relatively the least for the S. macrophylla stem bark extract at 500 ppm concentration after 48 h while it reached 100% and 95% in case of S. mahogani bark and S. macrophylla leaves, respectively. The% infestation with Varroa in colonies treated with the different extracts at various time intervals showed that the rate of infestation decreased to 0.0% after 12 days from the beginning of treatments with 500 ppm of S. mahogani leaves extract compared to 0.79% decrease after treatment with Mitac, a reference drug (60 mg/colony). The rate of infestation in case of treatments with S. mahogani bark, S. macrophylla leaves and S. macrophylla bark was decreased to 0.11%, 2.41% and 1.08%, respectively. The highest reduction was observed with S. mahogani leaves extract followed by S. mahogani bark. All the tested extracts showed less or no effect on honey bees at the different concentrations and at different bioassay times. This study suggested that the use of natural plant extracts or their products as ecofriendly biodegradable agents could be of high value for the control of Varroa mite. 相似文献
47.
vanKuyk PA Benen JA Wösten HA Visser J de Vries RP 《Applied microbiology and biotechnology》2012,93(1):285-293
AmyR is commonly considered a regulator of starch degradation whose activity is induced by the presence of maltose, the disaccharide
building block of starch. In this study, we demonstrate that the role of AmyR extends beyond starch degradation. Enzyme activity
assays, genes expression analysis and growth profiling on d-glucose- and d-galactose-containing oligo- and polysaccharides showed that AmyR regulates the expression of some of the Aspergillus niger genes encoding α- and β-glucosidases, α- and β- galactosidases, as well as genes encoding α-amlyases and glucoamylases. In
addition, we provide evidence that d-glucose or a metabolic product thereof may be the inducer of the AmyR system in A. niger and not maltose, as is commonly assumed. 相似文献
48.
Gutierres JM Kaizer RR Schmatz R Mazzanti CM Vieira JM Rodrigues MV Jaques J Carvalho F Zanini D Morsch VM Schetinger MR Spanevello RM 《Cell biochemistry and function》2012,30(4):286-292
α-Tocopherol (α-Toc) is involved in various physiologic processes, which present antioxidant and neuroprotective properties. High-fat diets have an important role in neurodegenerative diseases and neurological disturbances. This study aimed to investigate the effects of treatment with α-Toc and the consumption of high-fat diets on ectonucleotidase activities in synaptosomes of cerebral cortex, hippocampus and striatum of rats. Animals were divided into four different groups, which received standard diet (control), high-fat saturated diet (HF), α-Toc and high-fat saturated diet plus α-Toc (α-Toc + HF). High-fat saturated diet was administered ad libitum and α-Toc by gavage using a dose of 50 mg·kg(-1). After 3 months of treatment, animals were submitted to euthanasia, and cerebral cortex, hippocampus and striatum were collected for biochemical assays. Results showed that adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) hydrolysis in the cerebral cortex, hippocampus and striatum were decreased in HF in comparison to the other groups (P < 0·05). When rats that received HF were treated with α-Toc, the activity of the ectonucleotidases was similar to the control. ATP, ADP and AMP hydrolysis in the cerebral cortex, hippocampus and striatum were increased in the α-Toc group when compared with the other groups (P < 0·05). These findings demonstrated that the HF alters the purinergic signaling in the nervous system and that the treatment with α-Toc was capable of modulating the adenine nucleotide hydrolysis in this experimental condition. 相似文献
49.
Rajaraman Swaminathan Ramanujam Selvakumaran Pulikattil Okkuru Esmy P. Sampath Jacques Ferlay Vinoda Jissa Viswanathan Shanta Mary Cherian Rengaswamy Sankaranarayanan 《Cancer epidemiology》2009,33(5):325-331
Background: Cancer pattern data are rare and survival data are none from rural districts of India. Methods: The Dindigul Ambilikkai Cancer Registry (DACR) covering rural population of 2 millions in Dindigul district, Tamil Nadu state, South India, registered 4516 incident cancers during 2003–2006 by active case finding from 102 data sources for studying incidence pattern, of which, 1045 incident cancers registered in 2003 were followed up for estimating survival. House visits were undertaken annually for each registered case for data completion. Cancer pattern was described using average annual incidence rates and survival experience was expressed by computing observed survival by actuarial method and age-standardized relative survival (ASRS). Results: The average annual age-standardized rate per 100,000 of all cancers together was higher among women (62.6) than men (51.9) in DACR. The most common cancers among men were stomach (5.6), mouth (4.2) and esophagus (3.7). Cervical cancer (22.1) was ranked at the top among women followed by breast (10.9) and ovary (3.3). DACR incidence rates were lesser by at least two folds and 5-year survival were on par or lower than Chennai metropolitan registry for most cancers. Five-year age-standardized relative survival (%) in DACR was as follows: all cancers (29%), larynx (48), mouth (42), breast/tongue (38) and cervix (37). Conclusion: Cancer incidence was significantly lower, cancer patterns were markedly different and population-based cancer survival was lower in rural areas than urban areas thus providing valuable leads in estimating realistic cancer burden and instituting cancer control programs in India. 相似文献
50.
Michael S. Lee Rajkumar Bondugula Valmik Desai Nela Zavaljevski In-Chul Yeh Anders Wallqvist Jaques Reifman 《PloS one》2009,4(7)