全文获取类型
收费全文 | 388篇 |
免费 | 21篇 |
国内免费 | 3篇 |
出版年
2021年 | 4篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 7篇 |
2015年 | 12篇 |
2014年 | 7篇 |
2013年 | 18篇 |
2012年 | 22篇 |
2011年 | 19篇 |
2010年 | 18篇 |
2009年 | 17篇 |
2008年 | 24篇 |
2007年 | 24篇 |
2006年 | 15篇 |
2005年 | 20篇 |
2004年 | 12篇 |
2003年 | 19篇 |
2002年 | 8篇 |
2001年 | 15篇 |
2000年 | 8篇 |
1999年 | 17篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 6篇 |
1994年 | 5篇 |
1993年 | 2篇 |
1992年 | 9篇 |
1991年 | 9篇 |
1990年 | 4篇 |
1989年 | 8篇 |
1988年 | 2篇 |
1987年 | 8篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 6篇 |
1983年 | 5篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1974年 | 7篇 |
1973年 | 3篇 |
1972年 | 4篇 |
1971年 | 1篇 |
1970年 | 2篇 |
1968年 | 1篇 |
排序方式: 共有412条查询结果,搜索用时 78 毫秒
41.
每搏量变异度是动态的容量监测指标.机械通气患者心肺的相互作用是每搏量变异度的产生基础,通过动脉压力波形分析技术可以进行连续监测.每搏量变异度能够准确预测容量治疗反应,与静态的血流动力学参数相比,对于优化心输出量和组织氧供更有优势,但也存在一定的局限性.每搏量变异度受多种因素影响且不能用于自主呼吸和心律失常的患者.临床应用时应该综合考虑其影响因素,结合其他的指标和方法指导容量治疗. 相似文献
42.
43.
Stridsberg M Eriksson B Fellström B Kristiansson G Tiensuu Janson E 《Regulatory peptides》2007,139(1-3):80-83
OBJECTIVE: CgA has been shown to be an excellent marker for neuroendocrine tumours. However, there are two major drawbacks with CgA measurements; elevated levels are common in patients with decreased renal function and in patients on treatment with proton pump inhibitors. These problems are not seen with CgB measurements. We have recently presented the development of 13 region-specific radioimmunoassays for measurements of CgB. A region-specific assay was identified, which measured higher concentrations of CgB than the other assays and seemed to be very useful as a marker for neuroendocrine tumours. The aim of the present study was therefore to further explore the diagnostic potential of this assay in the clinical management of patients with neuroendocrine tumours. METHODS: Measurements of CgB with two methods were compared with CgA in plasma samples from patients investigated for neuroendocrine tumours (N=86), patients with decreased renal function (N=35) and patients on treatment with proton pump inhibitors (N=29). RESULTS: The diagnostic sensitivity for the new CgB assay was almost as good as that for CgA. Furthermore, with CgB measurements we could avoid the falsely elevated levels of CgA found in patients with decreased renal function and treatment with proton pump inhibitors. CONCLUSIONS: We conclude that the new CgB assay can serve as a complement to CgA measurements as an important tumour marker for neuroendocrine tumours. 相似文献
44.
The intra-annual variability of soft-bottom macrobenthos abundance patterns in the North Channel of the Seine estuary 总被引:2,自引:0,他引:2
Nicolas Desroy Anne-Laure Janson Lionel Denis Gregory Charrier Sandric Lesourd Jean-Claude Dauvin 《Hydrobiologia》2007,588(1):173-188
Temporal and spatial variability of the Abra alba–Pectinaria koreni and Macoma balthica communities was examined in the northern part of the Seine estuary (North Channel) over different space and time scales in
order to assess the role that the hydrologic regime and/or anthropogenic influences play in defining benthic communities over
time. Sediment in the North Channel displayed strong spatial and temporal variability, sustained by intense sediment transport
episodes. Total macrobenthic abundances ranged widely on the course of the year and there was no evidence of a seasonal signal
for the density fluctuations, whatever the spatial scale considered. The bio-sedimentary dynamics can be divided into two
periods: the first corresponds to the high flow rate period (January–May) during which fauna is influenced by fine silt/clay
deposition, and the second to the low flow rate period (June–December) during which sandy deposits prevail. Despite the absence
of significant correlations between sediment composition and abundance, episodes of sediment transport seem to be an important
structuring mechanism in the Seine estuary. As a consequence, the faunal composition varied throughout the year. The winter
and spring fauna, characterised by species living on muddy fine-sands or muds, were enriched during the summer and autumn
by species living in clean fine sand, such as Donax vittatus, Nephtys cirrosa or Spio decoratus, mainly represented by adult individuals. Secondary settlement of drifters may explain the rapid structuration of assemblages
a few days after the sandy deposits. Our results suggest the importance of the bentho-pelagic coupling, primarily induced
by the sedimentary instability, on the macrobenthic fauna dynamics. The intra-annual variability of assemblages at the mouth
of the Seine river and the silted situation of the North Channel might simply be the result of the silting up and alteration
of the inner estuary, generated by several decades of man-made modifications and natural processes. 相似文献
45.
白马雪山国家级自然保护区典型森林生态系统服务 总被引:1,自引:0,他引:1
生态系统服务是近年来生态学研究的热点领域,对关键区域生态系统服务的研究具有重要意义.云南省白马雪山国家级自然保护区地处青藏高原南延部分,拥有独特的地理位置,是生物多样性保护的热点区域.本文对该保护区森林生态系统的生物量与生产力、水源涵养、营养物质循环等3项服务的功能量进行了评估.结果表明:保护区森林总生物量2215.86×104t,生产力171.84×104t·a-1;水源涵养量11964.56×104m3;N、P、K年吸收量分别为26025.94t、2638.57t、12016.85 t.研究表明,保护区森林生态效益显著,对于维持当地以及周边地区的生态安全具有重要意义. 相似文献
46.
Dogterom M Kerssemakers JW Romet-Lemonne G Janson ME 《Current opinion in cell biology》2005,17(1):67-74
The assembly and disassembly of microtubules can generate pushing and pulling forces that, together with motor proteins, contribute to the correct positioning of chromosomes, mitotic spindles and nuclei in cells. In vitro experiments combined with modeling have shed light on the intrinsic capability of dynamic microtubules to generate force, and various observations of positioning processes in cells and model systems have shown how pushing and pulling forces are used in different situations. A sophisticated set of microtubule-end-binding proteins is responsible for steering dynamic microtubules toward their cellular target and regulating the pushing and/or pulling forces that are generated once contact is established. 相似文献
47.
Kawa Amin Christer Janson Lahja Sevéus Kaoru Miyazaki Ismo Virtanen Per Venge 《Respiratory research》2005,6(1):110
Background
Laminins are a group of proteins largely responsible for the anchorage of cells to basement membranes. We hypothesized that altered Laminin chain production in the bronchial mucosa might explain the phenomenon of epithelial cell shedding in asthma. The aim was to characterize the presence of Laminin chains in the SEBM and epithelium in allergic and non-allergic asthmatics.Patients and methods
Biopsies were taken from the bronchi of 11 patients with allergic and 9 patients with non-allergic asthma and from 7 controls and stained with antibodies against the Laminin (ln) chains alpha1-alpha5, beta1-beta2 and gamma1-gamma2.Results
Lns-2,-5 and -10 were the main Laminins of SEBM. The layer of ln-10 was thicker in the two asthmatic groups while an increased thickness of lns-2 and -5 was only seen in allergic asthmatics. The ln gamma2-chain, which is only found in ln 5, was exclusively expressed in epithelial cells in association with epithelial injury and in the columnar epithelium of allergic asthmatics.Conclusion
The uncoordinated production of chains of ln-5 in allergic asthma could have a bearing on the poor epithelial cell anchorage in these patients. 相似文献48.
CD4 T cell-dependent autoimmunity against a melanocyte neoantigen induces spontaneous vitiligo and depends upon Fas-Fas ligand interactions 总被引:5,自引:0,他引:5
Lambe T Leung JC Bouriez-Jones T Silver K Makinen K Crockford TL Ferry H Forrester JV Cornall RJ 《Journal of immunology (Baltimore, Md. : 1950)》2006,177(5):3055-3062
Better understanding of tolerance and autoimmunity toward melanocyte-specific Ags is needed to develop effective treatment for vitiligo and malignant melanoma; yet, a systematic assessment of these mechanisms has been hampered by the difficulty in tracking autoreactive T cells. To address this issue, we have generated transgenic mice that express hen egg lysozyme as a melanocyte-specific neoantigen. By crossing these animals to a hen egg lysozyme-specific CD4 TCR transgenic line we have been able to track autoreactive CD4+ T cells from their development in the thymus to their involvement in spontaneous autoimmune disease with striking similarity to human vitiligo vulgaris and Vogt-Koyanagi-Harada syndrome. Our findings show that CD4-dependent destruction of melanocytes is partially inhibited by blocking Fas-Fas ligand interactions and also highlights the importance of local control of autoimmunity, as vitiligo remains patchy and never proceeds to confluence even when Ag and autoreactive CD4+ T cells are abundant. Immune therapy to enhance or suppress melanocyte-specific T cells can be directed at a series of semiredundant pathways involving tolerance and cell death. 相似文献
49.
In this article we describe the behavioral responses of a group of white-faced sakis' (Pithecia pithecia) to fruit and water scarcity. Six sakis were observed on Round Island in Guri Lake, Venezuela, between March and May 1996. These months are considered the dry season and the beginning of the wet season. Sakis specialize in eating seeds. During the present study only one tree species, Licania discolor (Chrysobalanaceae), fruited in substantial numbers. Licania seeds accounted for 88% of the time the sakis spent eating fruit in March, 87% in April, and 80% in May. We estimate that the sakis' intake of Licania seeds dropped from 2,573 seeds in the 15-day observation period in March to 956 seeds in the 16-day observation period in May. The sakis not only spent less time eating Licania, they ate the seeds at a much slower rate. The drop in the sakis' feeding rate was probably due to increased local search and inspection times. In response to the scarcity of fruit, the sakis ate more young leaves, insects, and flowers. Feeding bouts became more frequent but shorter. Mean distances between feeding bouts fell significantly and the sakis revisited trees less often. 相似文献
50.
While debate continues as to whether genetic sequences, which many argue represent natural phenomena rather than inventions, should be subject to standard patent protections, issuance of patents that claim DNA sequences remains common practice. In an attempt to insulate researchers from patent claims that could hinder scientific progress, many countries have provided general exemptions for scientific research. However, there is no international consensus about the extent of required protections, and even existing exemptions vary widely in clarity and are limited in practical application. We believe that gene patents raise several unique issues that are inadequately handled by the current research exemptions. 相似文献