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291.
Sven Rossel Janna Peters Silke Laakmann Pedro Martínez Arbizu Sabine Holst 《Molecular ecology resources》2023,23(7):1620-1631
Morphological identification of cnidarian species can be difficult throughout all life stages due to the lack of distinct morphological characters. Moreover, in some cnidarian taxa genetic markers are not fully informative, and in these cases combinations of different markers or additional morphological verifications may be required. Proteomic fingerprinting based on MALDI-TOF mass spectra was previously shown to provide reliable species identification in different metazoans including some cnidarian taxa. For the first time, we tested the method across four cnidarian classes (Staurozoa, Scyphozoa, Anthozoa, Hydrozoa) and included different scyphozoan life-history stages (polyp, ephyra, medusa) in our dataset. Our results revealed reliable species identification based on MALDI-TOF mass spectra across all taxa with species-specific clusters for all 23 analysed species. In addition, proteomic fingerprinting was successful for distinguishing developmental stages, still by retaining a species specific signal. Furthermore, we identified the impact of different salinities in different regions (North Sea and Baltic Sea) on proteomic fingerprints to be negligible. In conclusion, the effects of environmental factors and developmental stages on proteomic fingerprints seem to be low in cnidarians. This would allow using reference libraries built up entirely of adult or cultured cnidarian specimens for the identification of their juvenile stages or specimens from different geographic regions in future biodiversity assessment studies. 相似文献
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Joanna Lazniewska Jack R. T. Darby Stacey L. Holman Alexandra Sorvina Sally E. Plush Massimiliano Massi Doug A. Brooks Janna L. Morrison 《Journal of biophotonics》2021,14(4):e202000322
Intrauterine growth restriction (IUGR) can result from reduced delivery of substrates, including oxygen and glucose, during pregnancy and may be caused by either placental insufficiency or maternal undernutrition. As a consequence of IUGR, there is altered programming of adipose tissue and this can be associated with metabolic diseases later in life. We have utilised two sheep models of IUGR, placental restriction and late gestation undernutrition, to determine the metabolic effects of growth restriction on foetal perirenal adipose tissue (PAT). Two-photon microscopy was employed to obtain an optical redox ratio, which gives an indication of cell metabolism. PAT of IUGR foetuses exhibited higher metabolic activity, altered lipid droplet morphology, upregulation of cytochrome c oxidase subunit genes and decreased expression of genes involved in growth and differentiation. Our results indicate that there are adaptations in PAT of IUGR foetuses that might be protective and ensure survival in response to an IUGR insult. 相似文献
294.
Pham Long Vo Janna Olmos Julian David Chernev Petko Kargul Joanna Messinger Johannes 《Photosynthesis research》2019,139(1-3):93-106
Photosynthesis Research - Photosynthetic water oxidation is catalyzed by the oxygen-evolving complex (OEC) in photosystem II (PSII). This process is energetically driven by light-induced charge... 相似文献
295.
Jack R. T. Darby Alexandra Sorvina Christie A. Bader Mitchell C. Lock Jia Yin Soo Stacey L. Holman Mike Seed Tim Kuchel Douglas A. Brooks Sally E. Plush Janna L. Morrison 《Journal of biophotonics》2020,13(3)
The primary metabolic pathway required to produce ATP differs as a result of tissue type, developmental stage and substrate availability. We utilized molecular and histological techniques to define the metabolic status in foetal and adult, adipose and skeletal muscle tissues. Redox ratios of these tissues were also determined optically by two‐photon microscopy. Adult perirenal adipose tissue had a higher optical redox ratio than fetal perirenal adipose tissue, which aligned with glycolysis being used for ATP production; whereas adult skeletal muscle had a lower optical redox ratio than fetal skeletal muscle, which aligned with oxygen demanding oxidative phosphorylation activity being utilized for ATP production. We have compared traditional molecular and microscopy techniques of metabolic tissue characterization with optical redox ratios to provide a more comprehensive report on the dynamics of tissue metabolism. 相似文献
296.
The temperature distribution inside a lossy sphere resulting from the absorption of microwave energy was approximated by successive numerical iterations, of the thermal energy equation. Heat transfer within the sphere by conduction was considered. In the model energy was not dissipated by convection but was contained in the sphere for over 200 seconds. Exposure of a 5-cm sphere to 3,000 MHz at 30 mW/cm2 for 200 seconds was calculated to produce a temperature rise of 0.56°C near the front surface. 相似文献
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Conclusion Much more work has been done on Pi transport processes, even in the last five years, than we have been able to mention in the space available. We have restricted our discussion to studies on mechanisms of transport or transport regulation, identification of transport proteins and their essential amino acids, and isolation, purification, and reconstitution of Pi transport systems. Many valuable studies on the physiology of Pi transport and its regulation and Pi transport in nonepithelial cells have also been conducted. Transport of Pi into and out of organelles other than the mitochondrion is gaining well-deserved attention, as are transport processes in fungi and plants. It is hoped that in another five years many Pi transport processes will be understood in true molecular terms and that this will increase our knowledge of cellular bioenergetics and metabolism. 相似文献